Transduction of Brain Neurons in Juvenile Chum Salmon (Oncorhynchus keta) with Recombinant Adeno-Associated Hippocampal Virus Injected into the Cerebellum during Long-Term Monitoring

Corpus cerebelli in juvenile chum salmon is a multiprojective region of the brain connected via afferent and efferent projections with the higher regions of the brainstem and synencephalon, as well as with multiprojection regions of the medulla oblongata and spinal cord. During the postembryonic development of the cerebellum in chum salmon, Oncorhynchus keta, the lateral part of the juvenile cerebellum gives rise to the caudomedial part of the definitive cerebellum, which is consistent with the data reported for zebrafish and mouse cerebellum. Thus, the topographic organization of the cerebellum and its efferents are similar between fish (chum salmon and zebrafish) and mammals, including mice and humans. The distributions of recombinant adeno-associated viral vectors (rAAVs) after an injection of the base vector into the cerebellum have shown highly specific patterns of transgene expression in bipolar neurons in the latero-caudal lobe of the juvenile chum tectum opticum. The distribution of rAAVs in the dorsal thalamus, epithalamus, nucleus rotundus, and pretectal complex indicates the targeted distribution of the transgene via the thalamo-cerebellar projections. The detection of GFP expression in the cells of the epiphysis and posterior tubercle of juvenile chum salmon is associated with the transgene’s distribution and with the cerebrospinal fluid flow, the brain ventricles and its outer surface. The direct delivery of the rAAV into the central nervous system by intracerebroventricular administration allows it to spread widely in the brain. Thus, the presence of special projection areas in the juvenile chum salmon cerebellum, as well as outside it, and the identification of the transgene’s expression in them confirm the potential ability of rAAVs to distribute in both intracerebellar and afferent and efferent extracerebellar projections of the cerebellum.     

降低单层混合建筑整体平移造价的技术措施

针对某单层混合结构建筑整体平移工程,提出在平移过程中保证其强度和刚度的同时又降低工程造价的具体措施,该措施与传统的房屋整体平移方法相比,简单、实用、工期短、强度和刚度易于控制、造价低。可为类似建筑整体平移工程提供参考。     

横向振动评估木结构建筑用规格材弹性性质

经Timoshenko梁方程推导出了以自振频率为变量的自由-自由梁横向振动弯曲弹性模量和剪切弹性模量的求解方程.通过对规格材动态振动和静态弯曲测试研究,发现用这种动态测试方法对规格材弹性性质进行预测效果较好,总回归方程相关系数在0.7以上,可以在实际中用于对足尺规格材的弹性性质进行动态评估.     

加强信息资源建设搭建数字化资源平台

文章对华北电网公司数字化图书馆的功能特点、资源建设情况及系统采用的关键技术等进行了简要介绍,从侧面论述了该数字化图书馆信息量大、专业性强、技术先进,是各类专业技术人员、管理人员日常工作中获取科技、管理知识的窗口和渠道。     

巨型球体钢结构工程焊接技术

根据安装顺序,严格执行焊接程序,消化创新了“鸟巢”钢结构合龙技术,实现了分层错45°合龙线的均匀布置,使合龙进展顺利;特别是根据主结构“统一对称、分区进行;自内而外、隔层焊接;分层合龙”的思想,焊接程序应当是:第一步,以柱为点,自内而外,分区进行,对称焊接;第二步,以梁为主,自内而外,先环后径,分区对称,逐步合龙;注意...     

Calibrating whole building energy models: An evidence-based methodology

This paper reviews existing case studies and methods for calibrating whole building energy models to measured data. This research describes a systematic, evidence-based methodology for the calibration of these models. Under this methodology, parameter values in the final calibrated model reference the source of information used to make changes to the initial model. Thus, the final model is based solely on evidence. Version control software stores a complete record of the calibration process, and the evidence on which the final model is based. Future users can review the changes made throughout the calibration process along with the supporting evidence. In addition to the evidence-based methodology, this paper also describes a new zoning process that represents the real building more closely than the typical core and four perimeter zone approach. Though the methodology is intended to apply to detailed calibration studies with high resolution measured data, the primary aspects of the methodology (evidence-based approach, version control, and zone-typing) are independent of the available measured data.     

使用后评估（POE）理论及案例研究——清华大学美术学院教学楼评价性后评估

使用后评估（post-ocupancy evaluation）是依据建筑性能标准对建筑环境进行评价与分析的操作。后评估以建筑使用者及其需求作为评价标准和依据,以期从评价中获取信息和经验为未来创造更好的建筑提供有益的借鉴。就当今建筑设计实践的全过程而言,使用后评估是建筑设计程序中的又一个重要的因素, 它的操作和研究使建成环境和设施更加完善,使建筑设计更加科学和合理。使用后评估分为三个层次,它们是指示性后评估、评价性后评估和诊断性后评估。本文通过对使用后评估理论的梳理,针对清华大学美术学院教学楼进行后评估,将后评估理论进行了实际程序的操作,为我国建筑环境后评估提供了案例研究。     

武汉丽人源生物科技发展有限公司产业园及无菌车间设计

通过对武汉丽人源生物科技发展有限公司园区及无菌车间设计的介绍,阐述工业园及工业厂房的设计思路,探讨其发展策略、发展方式和实施策略。     

对建筑业产业定位等若干问题的思考

建筑业是国民经济的支柱产业之一,应属国家的第二产业,但现实中却没有明确的产业定位。加之与此相关的法规缺失、制度滞后及某些行政管理错位,给建筑业的发展造成制约。建议国家有关部门要认真研究建筑业发展的问题,为建筑业发展创造良好的外部环境。     

江苏建筑职业技术学院图书馆空调系统三维绘图的运用

简要介绍了该工程空调系统设计方案。结合工程设计体会,总结了三维绘图软件的优势与不足,以及应用该软件后设计思路的调整。