Human catalase is imported and assembled in peroxisomes of Saccharomyces cerevisiae

To study the conservation of peroxisomal targeting signals, we have determined the intracellular localization of human peroxisomal catalase when expressed in yeast. Using immunofluorescence, differential centrifugation and immunoelectron microscopy, we show that the protein is targeted to the peroxisomes of the heterologous cell and assembled in its active tetrameric form. These data show the conservation of the catalase targeting signal and import specificity between human and yeast peroxisomes.     

Anaerobic digestion of alkaline black liquor using an up-flow anaerobic sludge blanket reactor

The anaerobic digestion of alkaline black liquor from a cereal straw pulping mill was studied in batch (serum bottles) and continuous systems (up-flow anaerobic sludge blanket reactor—UASB). The batch digestion studies confirmed that lignin and related compounds (LRC) in the alkaline black liquor were the main inhibitory substances and could not be decomposed by anaerobic bacteria. At organic loading rates of 5–10 kg COD m^?3 day^?1, the UASB reactor achieved 50–60% COD removal efficiencies. Gas production was 2–3 dm³ per dm³ of alkaline black liquor. Two different sludge types were examined in the reactor: granular and cluster-like sludges. Sludge in a cluster, which involved many small granules and flocs, tended to form larger aggregates and possessed good settling ability.     

Fe—Ga—O系平衡测定

在钼丝炉内氩气保护下，利用固体电解质定氧探头测定了含镓１．８７％～６．２１％范围内Ｆｅ－Ｇａ－Ｏ三元系平衡时的氧活度，通过处理得到镓在铁液中的标准溶解自由能等热力学参数与温度的关系。     

Foredune dynamics at a Lake Michigan site during rising and high lake levels

On Great Lakes dunes, the link between foredune dynamics and coastal processes is seen in dune responses to changing lake levels. This paper investigates foredune dynamics during a recent period of rising and high lake levels. The study location was an active foredune in P.J. Hoffmaster State Park on the east coast of Lake Michigan, where field data were collected from 2000 through the final destruction of the foredune by wave removal in November 2019. Foredune dynamics were studied with erosion pins, direct observations, photographs, mapping, and on-site wind measurements. Regional climate and lake-level data were obtained from established data collection programs. The response of the foredune to rising lake levels was compared to several models of foredune behavior. During the study, the Lake Michigan-Huron level rose 1.89 m from January 2013 to July 2020. After an early transitional period, foredune activity was characterized by scarp retreat (4–19 m per year) and dune narrowing from 2014 to 2019. When the foredune completely disappeared in November 2019, erosion/scarping began on the next landward dune. The foredune activity fits Olson’s (1958) model for foredune growth and erosion through lake-level cycles. The foredune migration predicted by the revised Davidson-Arnott (2021) model of foredune response to relative water level rise did not occur, most likely because the rate of lake-level rise was too high. The six years of foredune narrowing before wave erosion started affecting the next landward dune represent a time-lag in Lake Michigan dune history models of increased dune activity during high lake-level stands.     

3D human pose estimation in motion based on multi-stage regression

3D human pose estimation in motion is a hot research direction in the field of computer vision. However, the performance of the algorithm is affected by the complexity of 3D spatial information, self-occlusion of human body, mapping uncertainty and other problems. In this paper, we propose a 3D human joint localization method based on multi-stage regression depth network and 2D to 3D point mapping algorithm. First of all, we use a single RGB image as the input, through the introduction of heatmap and multi-stage regression to constantly optimize the coordinates of human joint points. Then we input the 2D joint points into the mapping network for calculation, and get the coordinates of 3D human body joint points, and then to complete the 3D human body pose estimation task. The MPJPE of the algorithm in Human3.6 M dataset is 40.7. The evaluation of dataset shows that our method has obvious advantages.     

Safe and intuitive manual guidance of a robot manipulator using adaptive admittance control towards robot agility

Key areas of robot agility include methods that increase capability and flexibility of industrial robots and facilitate robot re-tasking. Manual guidance can achieve robot agility effectively, provided that a safe and smooth interaction is guaranteed when the user exerts an external force on the end effector. We approach this by designing an adaptive admittance law that can adjust its parameters to modify the robot compliance in critical areas of the workspace, such as near and on configuration singularities, joint limits, and workspace limits, for a smooth and safe operation. Experimental validation was done with two tests: a constraint activation test and a 3D shape tracing task. In the first one, we validate the proper response to constraints and in the second one, we compare the proposed approach with different admittance parameter tuning strategies using a drawing task where the user is asked to guide the robot to trace a 3D profile with an accuracy or speed directive and evaluate performance considering path length error and execution time as metrics, and a questionnaire for user perception. Results show that appropriate response to individual and simultaneous activation of the aforementioned constraints for a safe and intuitive manual guidance interaction is achieved and that the proposed parameter tuning strategy has better performance in terms of accuracy, execution time, and subjective evaluation of users.     

Human error identification in human reliability assessment. Part 2: Detailed comparison of techniques

This is the second part of a two-part review of human error identification (HEI) approaches in human reliability assessment (HRA). Part 1 reviewed the probabilistic risk assessment (PRA) context in which HRA occurs, and then detailed 12 HEI techniques which have evolved in the field of HRA. Part 2 attempts to compare the way these techniques perform against a range of criteria relevant to HEI theoretical and empirical validity, and practical usefulness in applied HRA. It is hoped that these comparisons will help assessors in the selection of techniques for practical applications. The comparisons also point to research and development needs in the area of applied HEI.     

小波域最小嵌入失真函数设计及其在隐写中的应用

为了提高隐写方案的安全性,提出一种基于最小嵌入失真原理和网格码的图像隐写算法。首先在离散小波域结合人眼视觉特性和整数提升小波变换设计了失真测度函数,主要考虑了亮度、频率和纹理掩蔽因子对载体失真的影响。然后结合网格码设计了隐写算法,将嵌入信息对载体的修改最小化并且集中在人眼不敏感区域。实验结果显示,方案具有良好的视觉不可见性,且能抵抗空域、小波域等隐写分析的攻击,安全容量达到0.4 bits/pixel。     

Microstructured Lipid Carriers (MLC) Based on N-Acetylcysteine and Chitosan Preventing Pseudomonas aeruginosa Biofilm

The aim of this work was the development of microstructured lipid carriers (MLC) based on chitosan (CH) and containing N-acetylcysteine (NAC), a mucolytic and antioxidant agent, to inhibit the formation of Pseudomonas aeruginosa biofilm. MLC were prepared using the high shear homogenization technique. The MLC were characterized for morphology, particle size, Z potential, encapsulation efficiency and drug release. The antioxidant properties of NAC-loaded microstructured carriers were evaluated through an in vitro spectrophotometer assay. Finally, the activity of NAC-CH-MLC on biofilm production by Pseudomonas aeruginosa was also evaluated. Results obtained from this study highlighted that the use of chitosan into the inner aqueous phase permitted to obtain microstructured particles with a narrow size range and with good encapsulation efficiency. NAC-loaded MLC showed higher antioxidant activity than the free molecule, demonstrating how encapsulation increases the antioxidant effect of the molecule. Furthermore, the reduction of biofilm growth resulted extremely high with MLC being 64.74% ± 6.2% and 83.74% ± 9.95%, respectively, at 0.5 mg/mL and 2 mg/mL. In conclusion, this work represents a favorable technological strategy against diseases in which bacterial biofilm is relevant, such as cystic fibrosis.     

Secondary Metabolic Profile as a Tool for Distinction and Characterization of Cultivars of Black Pepper (Piper nigrum L.) Cultivated in Pará State,Brazil

This study evaluated the chemical compositions of the leaves and fruits of eight black pepper cultivars cultivated in Pará State (Amazon, Brazil). Hydrodistillation and gas chromatography–mass spectrometry were employed to extract and analyze the volatile compounds, respectively. Sesquiterpene hydrocarbons were predominant (58.5–90.9%) in the cultivars “Cingapura”, “Equador”, “Guajarina”, “Iaçará”, and “Kottanadan”, and “Bragantina”, “Clonada”, and “Uthirankota” displayed oxygenated sesquiterpenoids (50.6–75.0%). The multivariate statistical analysis applied using volatile composition grouped the samples into four groups: γ-Elemene, curzerene, and δ-elemene (“Equador”/“Guajarina”, I); δ-elemene (“Iaçará”/“Kottanadan”/“Cingapura”, II); elemol (“Clonada”/“Uthirankota”, III) and α-muurolol, bicyclogermacrene, and cubebol (“Bragantina”, IV). The major compounds in all fruit samples were monoterpene hydrocarbons such as α-pinene, β-pinene, and limonene. Among the cultivar leaves, phenolics content (44.75–140.53 mg GAE·g⁻¹ FW), the enzymatic activity of phenylalanine-ammonia lyase (20.19–57.22 µU·mL⁻¹), and carotenoids (0.21–2.31 µg·mL⁻¹) displayed significant variations. Due to black pepper’s susceptibility to Fusarium infection, a molecular docking analysis was carried out on Fusarium protein targets using each cultivar’s volatile components. F. oxysporum endoglucanase was identified as the preferential protein target of the compounds. These results can be used to identify chemical markers related to the susceptibility degree of black pepper cultivars to plant diseases prevalent in Pará State.