滨水型居住景观生态环境规划——以福州乌龙江住区规划为例

城市滨水区作为城市中人类活动与自然过程共同作用最为重要的地带之一,其规划涉及多学科多方面的问题,以福州乌龙江滨水住区的景观设计为例,阐述进行城市滨水区生态、功能、经济等方面中设计的一些理念与方法。     

承载运河文化，复兴滨水公园-南通市通吕运河（船闸东路段）景观设计

作为南通市第一运河的起点和南通市港闸区发展重地,通吕运河——船闸东路段景观规划直接影响着城市区域的发展。在尊重通吕运河的历史文化和保护生态环境的前提下,提炼运河历史文化的设计元素,为南通展现一个现代城市滨水空间,是整个项目的重要宗旨。该文通过研究运河文化的内涵,结合南通市船闸东路——通吕运河景观设计实例,详细讨论了运河文化元素在城市滨水景观的应用。     

忆羊城八景--广州古今山水城市历史研究

广州城市历史悠久,源远流长。其独特的岭南文化风采和环境风貌形成了广州特有的历史文明特色。随着广州城市化进程的迅速推进,许多颇具自然景观的地段在大开发、大建设中遭到破坏和摧残。面对广州城市化带来的弊端,人们很自然地对古代广州山水城市的美景产生一种特殊的怀念和眷恋。该文通过研究广州古今城市的山水景观状况,分析各个时期的广州山水特色与羊城八景的发展与变化,旨在探讨古代广州山水城市的形成原因与创造哲理,为今天创造具有岭南特色的广州城市山水环境及城市景观文化提供重要依据。     

外滩隧道工程景观设计的整体风格构思和要点

外滩是上海的一张景观名片,面对着开阔的母亲河——黄浦江,背倚着造型严谨、风格迥异、有"万国建筑博览群"美誉的外滩建筑群。外滩隧道由南向北穿越了整个外滩。代表现代交通科技的隧道工程,介绍了设计中对外滩隧道工程景观的整体风格构思,并深入分析了隧道出入口、内景、隧道风塔、装饰材料选择等景观设计要点。     

浅析城市滨水景观的特色设计

从景观内 容设 置 、组织 及 节点 等 方面 对城 市 滨水 区景 观 的特 色设 计 进行 分析 。     

固相萃取净化-气相色谱法检测人乳中 持久性有机氯农药

目的 建立母乳中有机氯农药六六六和滴滴涕的检测方法, 并对北京、兰州和杭州三个地区的蓄积水平现状进行分析。方法 母乳样品用乙腈提取, 经过酸化的硅胶固相萃取柱净化, 浓缩后, 采用气相色谱仪检测。结果 8种农药的线性范围在5~200 ng/mL, 相关系数0.9955~0.9994, 定量限均小于2.6 μg/kg, 平均回收率在70%~122%, 相对标准偏差在3.91%~10.77%。结论 本方法结合磺化法和固相萃取法, 能有效去除基质中的杂质。通过方法学验证证明, 本方法操作简单、精确、快速, 适用于母乳中有机氯的检测。随着六六六、滴滴涕等持久性有机氯农药的禁用或严格限用, 母乳中持久性有机氯农药的蓄积水平明显降低。     

Livin在人喉癌细胞中的表达及白毛藤多糖的干预作用

研究白毛藤多糖对人喉癌细胞生长的抑制作用以及Livin表达的影响。将白毛藤多糖以不同浓度加入到人喉癌细胞,用M1Tr比色法检测细胞增殖抑制率;荧光定量PCR检测LivinmRNA的表达。结果表明,白毛藤多糖可诱导Livin基因的表达,Livin可能参与白毛藤多糖抑制人喉癌细胞的增殖。     

The interaction of sesamol with DNA and cytotoxicity,apoptosis, and localization in HepG2 cells

Sesamol, a nutritional antioxidant phenolic compound present in sesame seed, has a potential therapeutic molecule effect against cancers. In this study, the interaction between sesamol and DNA was investigated by employing ultraviolet/visible (UV/Vis), fluorescence, circular dichroism (CD), Fourier transform infrared spectroscopy (FT-IR), and molecular modeling. The fluorescence analysis indicated that the fluorescence quenching mechanism of sesamol by calf thymus DNA (ctDNA) occurred through static quenching. The UV/Vis, CD, FT-IR spectra and molecular docking results implied that the primary binding mode was minor groove binding. Furthermore, the intracellular interaction of sesamol with DNA and its bioactivity effect were explored. The cell activity results demonstrated that sesamol induced hepatic cell line (HepG2) death. The acridine orange (AO)/ethidium bromide (EB) staining assay and DNA fragmentation confirmed that sesamol could efficiently induce the apoptosis of HepG2 cells. Moreover, addition of sesamol to HepG2 cells resulted in nuclear localization, as visualized by confocal microscopy.     

Effects of ready to drink teas on AhR- and PXR-mediated expression of cytochromes P450 CYP1A1 and CYP3A4 in human cancer cell lines and primary human hepatocytes

A variety of xenobiotics are taken in the diet and they can interfere with regulatory pathways of drug metabolizing enzymes in humans. This can result in food-drug interactions, which is undesirable clinical situation where drug pharmacokinetics are influenced by dietary compounds. Xenobiotics-mediated food-drug interactions include the induction of drug metabolizing cytochromes P450. The expression of the most important inducible cytochromes CYP1A and CYP3A4 are regulated by xenoreceptors PXR and AhR.We examined extracts from 17 different flavoured ready to drink teas (RDTs) for their capabilities to activate PXR and AhR receptors and to induce CYP3A4 and CYP1A genes. Primary cultures of human hepatocytes and cancer cell lines HepG2 and LS174T were used as in vitro models. Gene reporter assays, RT-PCR and Western blots were performed.We identified three RDTs that induced CYP3A4 mRNA and protein, implying a potential for food-drug interactions. Several RDTs slightly elevated CYP1A1 expression or activated AhR.     

食用菌酶解改性型膳食纤维对人体机能影响研究

为了研究食用菌酶解改性型膳食纤维对人体机能的影响,文章对食用菌膳食纤维进行了酶解改性处理,并分析其酶解改性后膳食纤维的性质特征,同时设定相应的试验环节分析其对人体机能的影响。通过分析该酶解前后的膳食纤维功能性质发现,酶解后得到的膳食纤维表面结构发生变化,结构层次感加强,空隙由密实走向稀松。基于膳食纤维结构的变化,其对葡萄糖、胆固醇的吸附能力均得到显著的增强,同时抗胃酸消化能力得到提升。通过多组性质测定可以看出,酶解后膳食纤维调节肠道菌群作用得到提升,对人体肠道乳酸含量具有间接调节功能,有助于促进肠道蠕动。在后续的研究中,将主要对此类膳食纤维的应用途径进行研究。