台湾华山1914文化产业创意园城市工业景观的重塑

伴随工业生产活动的迁移或废止,场址中废置的工业建筑及附属设施等成为现代城市宝贵的工业遗迹,并作为人类文明和城市发展的见证,成为人类历史的重要媒介和遗留的文化景观。对其保护及再利用以实现城市工业景观的重塑与再生已成为当代城市更新的重要内容和城市发展的新议题。以城市工业景观重塑与再生的相关概念、要点及实践为理论基础,结合台湾华山1914文创园区案例,论当下城市工业景观的重塑与再生。     

宜将新绿付老枝——蛇口南海意库3#楼改造设计

介绍蛇口南海意库3#楼改造的绿色实践,该项目实现了资源的循环利用和绿色建筑技术的有机运用。指出为节约资源,应提倡在不拆除原有建筑的情况下实现产业的转型并付诸实践,让原有的建筑适应新的使用功能。     

现代设计理念在传统建筑重塑中的应用

现代设计理念在传统建筑重塑中的应用,不是简单的照搬,是正视早已变化的材料、功能需求和审美需求;是去尝试成功解读传统建筑装饰语言,要注重形式与功能的统一、重视部分与整体的协同关系;是不断地摸索和创新。     

氯气压缩机扩能改造总结

介绍了氯气压缩机扩能改造方案,并结合实际,提出了改造过程中的注意事项.     

Generation of glycerophospholipid molecular species in the yeast Saccharomyces cerevisiae. Fatty acid pattern of phospholipid classes and selective acyl turnover at sn-1 and sn-2 positions

Acyl chains linked to phospholipids of the yeast, Saccharomyces cerevisiae, are mainly C16:1 and C18:1 accompanied by minor amounts of C14:0, C16:0 and C18:0. In view of this rather simple fatty acid composition, the question arose whether in yeast, as in higher eukaryotes, fatty acyl groups were characteristically distributed among the sn-1 and sn-2 positions of distinct phospholipid classes. Analysis of fatty acids linked to the sn-1 and sn-2 positions of the major phospholipids showed that indeed saturated fatty acyl groups predominated in the sn-1 positions. While the percentage of saturated fatty acids was low (10%) in phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) from cells grown on rich medium, it was higher in phosphatidylserine (PtdSer) (25%) and highest in phosphatidylinositol (PtdIns) (41%). Oleate was mainly linked to position sn-2, while palmitoleate predominated in position sn-1. Striking differences in the fatty acid distribution of phospholipids that are metabolically closely related (e.g. PtdSer and PtdEtn, PtdEtn and PtdCho, and PtdIns and PtdSer) suggest that pathways must exist for the generation of distinct phospholipid molecular species within the different phospholipid classes. The highly selective incorporation of exogenous [¹⁴C]palmitic acid (90%) and [³H]oleic acid (99%) into the sn-2 position of PtdCho, and the preferential incorporation of these fatty acids into the sn-2 position of PtdEtn (70 and 90%, respectively, for palmitic and oleic acid) are compatible with the postulate that phospholipase A₂-mediated deacylation followed by reacylation of the lysophospholipids is involved in the generation of phospholipid species in yeast.     

Microvascular growth, development, and remodeling in the embryonic avian kidney: the interplay between sprouting and intussusceptive angiogenic mechanisms

Embryonic development is associated with extensive vascular growth and remodeling. We used immunohistochemical, light and electron microscopical techniques, as well as vascular casting methods to study the developing chick embryo kidney with special attention to the interplay between sprouting and intussusceptive vascular growth modes. During inauguration at embryonic day 5 (E5), the early mesonephros was characterised by extensive microvascular sprouting. By E7, the vascular growth mode switched to intussusception, which contributed to rapid kidney vasculature growth up to E11, when the first obvious signs of vascular degeneration were evident. The metanephros underwent similar phases of vascular development inaugurating at E8 with numerous capillary sprouts and changing at E13 to intussusceptive growth, which was responsible for vascular amplification and remodeling. A phenomenal finding was that future renal lobules arose as large glomerular tufts, supplied by large vessels, which were split into smaller intralobular feeding and draining vessels with subsequent formation of solitary glomeruli. This glomerular duplication was achieved by intussusception, i.e., by formation of pillars in rows and their successive merging to delineate the vascular entities. Ultimately, the maturation of the vasculature was achieved by intussusceptive pruning and branching remodeling. An interesting finding was that strong VEGF expression was associated with the sprouting phase of angiogenesis while bFGF was upregulated during the phase of intussusceptive microvascular growth. We conclude that microvascular growth and remodeling in avian kidney follows an adroitly crafted pattern, which entails a precise spaciotemporal interplay between sprouting and intussusceptive angiogenic growth modes supported partly by VEGF and bFGF.     

Quantification of Dendritic Spines Remodeling under Physiological Stimuli and in Pathological Conditions

Numerous brain diseases are associated with abnormalities in morphology and density of dendritic spines, small membranous protrusions whose structural geometry correlates with the strength of synaptic connections. Thus, the quantitative analysis of dendritic spines remodeling in microscopic images is one of the key elements towards understanding mechanisms of structural neuronal plasticity and bases of brain pathology. In the following article, we review experimental approaches designed to assess quantitative features of dendritic spines under physiological stimuli and in pathological conditions. We compare various methodological pipelines of biological models, sample preparation, data analysis, image acquisition, sample size, and statistical analysis. The methodology and results of relevant experiments are systematically summarized in a tabular form. In particular, we focus on quantitative data regarding the number of animals, cells, dendritic spines, types of studied parameters, size of observed changes, and their statistical significance.     

In Vivo Silencing of Genes Coding for dTip60 Chromatin Remodeling Complex Subunits Affects Polytene Chromosome Organization and Proper Development in Drosophila melanogaster

Chromatin organization is developmentally regulated by epigenetic changes mediated by histone-modifying enzymes and chromatin remodeling complexes. In Drosophila melanogaster, the Tip60 chromatin remodeling complex (dTip60) play roles in chromatin regulation, which are shared by evolutionarily-related complexes identified in animal and plants. Recently, it was found that most subunits previously assigned to the dTip60 complex are shared by two related complexes, DOM-A.C and DOM-B.C, defined by DOM-A and DOM-B isoforms, respectively. In this work, we combined classical genetics, cell biology, and reverse genetics approaches to further investigate the biological roles played during Drosophila melanogaster development by a number of subunits originally assigned to the dTip60 complex.     

Tissue-Engineered Vascular Grafts: Emerging Trends and Technologies

Vascular tissue engineering has made prodigious progress in recent years by converging multidisciplinary approaches. Latest technological advancements foster the development of next-generation tissue-engineered vascular grafts (TEVGs) for treating various vasculopathies. While traditional therapeutic methods rely on bypassing the severely damaged vessels with synthetic counterparts with no growth potential, contemporary perspectives focus on biodegradable conduits bestowing an inherent remodeling capability. This review highlights emerging innovative trends and technologies adopted to pragmatically fulfill current scientific needs while improving overall TEVG performance in pre-clinical and clinical settings. A comprehensive overview of various milestones achieved in the past few decades is first summarized, followed by an appraisal of the significant hurdles for clinical translation. The latest techniques to rationally address critical challenges, viz., intimal hyperplasia, thrombosis, constructive graft remodeling, and adequate neo-tissue formation are discussed. Finally, an update on ongoing clinical trials is provided and future perspectives required to persuade TEVGs to become a clinical reality are delineated.     

燕麦蒽酰胺生理功能研究进展

目的 探究尾静脉注射短链酰基辅酶A脱氢酶(SCAD)重组腺病毒能否改善自发性高血压大鼠(SHR)血管重构。方法 实验分为6组:Wistar+NS组、Wistar+GFP组、Wistar+Ad-SCAD组、SHR+NS组、SHR+GFP组和SHR+Ad-SCAD组。腺病毒包装的SCAD和GFP纯化后,以尾静脉方式注射给药8周。采用超声心动图检测大鼠心功能情况；采用无创血压仪检测大鼠的血压变化；主动脉HE染色、天狼星红染色、DHE染色、TUNEL染色、EVG染色,观察血管重构。采用实时荧光定量PCR检测相关基因mRNA表达变化、Western blot检测相关蛋白表达变化,观察游离脂肪酸、一氧化氮(NO)和ATP含量变化。结果 (1)尾静脉注射SCAD重组腺病毒,大鼠主动脉中SCAD蛋白出现过表达,mRNA水平增高,SCAD酶活性增加；(2)在病理状态下,增加主动脉中SCAD表达,可以降低血压,改善心功能,改善血管管腔大小,减少胶原沉积,减少血管活性氧(ROS)的生成和细胞凋亡；(3)在病理状态下,SCAD的表达增加可减少血清和主动脉中游离脂肪酸的含量,增加组织中的ATP水平,激活内皮型一氧化氮合酶(eNOS)磷酸化,增加主动脉NO的生成。结论 自发性高血压大鼠主动脉SCAD的表达增加能逆转高血压血管重构,可能与其减少血清中游离脂肪酸的含量、增加NO水平、减少活性氧生成、消除氧化应激有关。