Structural arrangement of the cardiac collagen fibers of healthy and diabetic dogs

The heart is composed by a specialized muscle, whose form and function are essentials for an adequate work and shows an amount of connective tissue which support and provide insertion for this muscle, whose collagen fibers are responsible for determination of tissue feature. Our objective was to identify the structural arrangement of the heart collagen fibers in dogs. The hearts of the dogs were submitted to the process of the controlled digestion with NaOH solution and observed by scanning electron microscope. Our results showed that the collagen fibers of the endomysial wall have structural arrangement composed by an irregular network with one layer in normal dogs but in diabetic dogs the network acquires a greater amount of the fibers and layers, looking like a "rug" of fibers modifying the relationships of the stress/strain of the tissue. Ahead of the observed results we are able to conclude that exist increase in the amount and thickness of cardiac collagen fibers, beyond the increase of layers and architectural disarrangement in the endomysial wall in the diabetic dogs.     

从信义大厦临街立面改造看高层烂尾楼问题

近年来,越来越多的烂尾楼改造工程受到重视,很多成功的经验表明,烂尾楼的再生利用可以取得显著的经济效益和社会效益。本文以重庆市渝中区朝天门地区信义大厦临街立面改造为例,分析烂尾楼改造的各种相关问题。     

Ion Channels and Transporters in Muscle Cell Differentiation

Investigations on ion channels in muscle tissues have mainly focused on physiological muscle function and related disorders, but emerging evidence supports a critical role of ion channels and transporters in developmental processes, such as controlling the myogenic commitment of stem cells. In this review, we provide an overview of ion channels and transporters that influence skeletal muscle myoblast differentiation, cardiac differentiation from pluripotent stem cells, as well as vascular smooth muscle cell differentiation. We highlight examples of model organisms or patients with mutations in ion channels. Furthermore, a potential underlying molecular mechanism involving hyperpolarization of the resting membrane potential and a series of calcium signaling is discussed.     

复方七芍降压片对自发性高血压大鼠肠系膜动脉中Ⅰ、Ⅲ型胶原蛋白表达及血压的影响

目的:探究复方七芍降压片对自发性高血压大鼠（SHR）血压及血管重塑的作用机制。方法:SHR大鼠随机分为模型组、厄贝沙坦片组、复方七芍降压片组,每组10只,另设10只WKY大鼠为正常对照组,给药6周,无创血压仪测量各周大鼠血压变化;HE染色观察大鼠肠系膜动脉病理变化并测量管壁厚度、血管中膜厚度及相同位置的管腔直径,比值记为WT;免疫组化法检测肠系膜动脉中基质金属蛋白酶-2（MMP-2）及Ⅰ、Ⅲ型胶原蛋白（COⅠ、COⅢ）表达情况。结果:给药6周后,与正常对照组比较,模型组大鼠各周血压均明显升高（P<0.01）,管壁厚度及WT值增高（P<0.01或P<0.05）,肠系膜动脉中MMP-2及Ⅰ、Ⅲ型胶原蛋白表达均明显升高（P<0.01）;与模型组比较,复方七芍降压片组大鼠各周血压均明显降低（P<0.01）,管壁厚度及WT值降低（P<0.05）,肠系膜动脉中MMP-2及Ⅰ、Ⅲ型胶原蛋白表达均明显降低（P<0.05）,且病理变化得到改善。结论:复方七芍降压片具有降低SHR大鼠血压,改善血管重塑的作用,其机制可能与降低MMP-2表达及Ⅰ、Ⅲ型胶原蛋白沉积有关。     

阿托伐他汀对自发性高血压大鼠心室重构的影响

目的: 观察阿托伐他汀对自发性高血压大鼠(SHR)心室重构的影响。方法: 24只SHR 随机分为4组,每组6只。SHR对照组、阿托伐他汀50mg组(50mg·kg^-1·d^-1)、阿托伐他汀10mg组(10 mg·kg^-1·d^-1)和缬沙坦组(20mg·kg^-1·d^-1);6只Wistar-Kyoto 大鼠(WKY)作为正常对照组。灌胃给药共6 周,分别于给药前和给药后每2 周测量大鼠尾动脉收缩压(SBP)。酶法测定血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)含量,放免法测定血浆和心肌血管紧张素Ⅱ(Ang Ⅱ)水平,并检测心肌羟脯氨酸、胶原蛋白含量和全心重量(HW)、左室重量(LVM)及左室重量指数(LVMI)。透射电镜观察心肌超微结构改变。结果: 用药前SHR 各组SBP均显著高于WKY 正常对照组(P<0.01),给药后第4、6 周,阿托伐他汀50 mg 组SBP明显下降(P<0.01),阿托伐他汀10 mg 组不明显;缬沙坦组自给药后第2 周,SBP明显下降(P<0.01)。阿托伐他汀50 mg 组TC、TG 及LDL-C水平较SHR 对照组明显降低(P<0.05或P<0.01),阿托伐他汀10 mg 组仅LDL-C 水平明显下降(P<0.05)。SHR对照组血浆Ang Ⅱ浓度与WKY 正常对照组比较无显著性差异,但心肌Ang Ⅱ浓度明显增高(P<0.05);给药6 周后,阿托伐他汀各剂量组和缬沙坦组血浆Ang Ⅱ浓度显著高于SHR 对照组(P<0.01),而心肌Ang Ⅱ 浓度在阿托伐他汀50 mg 组和缬沙坦组明显降低(P<0.05)。SHR 对照组心肌羟脯氨酸和胶原蛋白含量较WKY 正常对照组明显升高(P<0.01);6 周后,阿托伐他汀50 mg 组较SHR 对照组降低(P<0.05)。SHR组HW、LVM和LVMI与WKY正常对照组相比增高(P<0.01),而阿托伐他汀50 mg 组却低于SHR 对照组(P<0.05)。透射电镜观察心肌超微结构显示,阿托伐他汀50mg组和缬沙坦组与SHR对照组比较,心肌细胞核膜较完整,肌原纤维清晰,排列较整齐,横纹清楚,间质胶原纤维无明显增生。结论: 阿托伐他汀能明显改善SHR 的心室重构,降低血压和心肌Ang Ⅱ浓度可能为其机制之一。     

氯气压缩机扩能改造总结

介绍了氯气压缩机扩能改造方案,并结合实际,提出了改造过程中的注意事项.     

台湾华山1914文化产业创意园城市工业景观的重塑

伴随工业生产活动的迁移或废止,场址中废置的工业建筑及附属设施等成为现代城市宝贵的工业遗迹,并作为人类文明和城市发展的见证,成为人类历史的重要媒介和遗留的文化景观。对其保护及再利用以实现城市工业景观的重塑与再生已成为当代城市更新的重要内容和城市发展的新议题。以城市工业景观重塑与再生的相关概念、要点及实践为理论基础,结合台湾华山1914文创园区案例,论当下城市工业景观的重塑与再生。     

An oligodeoxynucleotide with promising modulation activity for the proliferation and activation of osteoblast

The paper explored the regulatory role of oligodeoxynucleotides (ODNs) with specific sequences in the proliferation and activation of osteoblast, using human osteoblast-like cell line MG 63 as the model. Through the administration of ODNs to MG 63 cells at a concentration of 1.0 μg/mL, ODN MT01 with positive effects on proliferation and activation of osteoblast was selected from 11 different ODNs by methyl thiazolyl tetrazolium (MTT) assay and alkaline phosphatase (ALP) activity measurement. To get a deeper insight into the molecular mechanism, effects of ODN MT01 treatment on the expression level of Sp7, runx-2, collagen-I, osteoprotegerin (OPG) and RANK ligand (RANKL) were determined using quantitative real time PCR and Western blotting. Remarkably, the mRNA and protein expression levels of Sp7, runx-2, collagen-I and OPG were improved after ODN MT01 treatment. Meanwhile, the protein expression level of RANKL was dramatically decreased. These results suggested that ODN MT01 had a significant impact in facilitating osteogenic proliferation and activation, and provided a direct evidence for the notion that single strand ODN could regulate the balance of bone formation and resorption, and thus was of great potential in the rebuilding of alveolar bone.     

Combination BMSC and Niaspan Treatment of Stroke Enhances White Matter Remodeling and Synaptic Protein Expression in Diabetic Rats

Objective

White matter remodeling plays an important role in neurological recovery after stroke. Bone marrow stromal cells (BMSCs) and Niaspan, an agent which increases high density lipoprotein (HDL), each induces neurorestorative effects and promotes white matter remodeling after stroke in non-diabetic rats. In this study, we test whether combination of BMSCs with Niaspan induces an enhanced white matter remodeling in the ischemic brain of diabetic rats.

Research design and methods

Type-1 diabetes (T1DM) rats were subjected to transient middle cerebral artery occlusion (MCAo) and treated with or without BMSCs; Niaspan; and the combination of BMSCs + Niaspan daily for 14 days after MCAo. Immunostaining for white matter remodeling and synaptic protein expression including NG2; CNPase; BS (Bielschowsky silver); LFB (luxol fast blue); Synaptophysin and SMI-31 immunostaining were performed.

Results

BMSC monotherapy did not regulate NG2 and CNPase expression compared to T1DM control rats. Both, combination of BMSCs + Niaspan treatment, and Niaspan monotherapy significantly increase NG2 and CNPase expression compared to T1DM control. While combination BMSC+Niaspan, BMSC monotherapy and Niaspan monotherapy groups all increase BS, LFB, synaptophysin, and SMI-31 expression in the ischemic brain compared to T1DM-MCAo control. In addition, the combination treatment significantly enhances LFB, SMI-31, and Synaptophysin expression compared to BMSC monotherapy.

Conclusions

Combination treatment of stroke with BMSCs and Niaspan in T1DM rats increases white matter remodeling and additively increases BMSC monotherapy induced myelination and synaptic plasticity after stroke in T1DM rats.     

Cardiac stromal cells on stage: From dull filler to specialized actors

Cardiac stromal cells have faced through the years a significant evolution in their definitions concerning theirphenotypes, markers, and functions. They are surging to key roles in physiopathology, becoming important targets to beexploited for cardiac repair. In this perspective, we briefly discuss their role in novel therapeutic strategies for enhancingcardiac repair and regeneration.