Myoglobin/gold nanoparticles/carbon spheres 3-D architecture for the fabrication of a novel biosensor

A novel biosensor based on a myoglobin/gold nanoparticles/carbon spheres (Mb-AuNPs-CNs) 3-D architecture bioconjunction has been fabricated for the determination of hydrogen peroxide (H₂O₂). Cyclic voltammetry (CV), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) were used to characterize the bioconjunction of the AuNPs-CNs with Mb. Experimental results demonstrate that the AuNPs-CNs hybrid material is more effective in facilitating electron transfer of the immobilized enzyme than CNs alone, which can be attributed to the unique nanostructure and larger surface area of the bioconjunction. The biosensor displayed good performance for the detection of H₂O₂ with a wide linear range from 0.28 μmol/L to 116.5 μmol/L and a detection limit of 0.12 μmol/L. The Michaelis-Menten constant K _M^app value was estimated to be 0.3 mmol/L. The resulting biosensor exhibited fast amperometric response, and good stability, reproducibility, and selectivity to H₂O₂. This article is published with open access at Springerlink.com     

Direct Digital Manufacturing Industry Ascendant

The paper introduces the origin of the word of Direct Digital Manufacturing and other forms of address, and the working principles of Direct Digital Manufacturing technology and major types of the technology, hard- ware and software development, use of materials, applications, market growth and its development prospects. Focused presentations of Direct Digital Manufacturing （additive manufacturing） compared to traditional mechani- cal manufacturing industry in the use of prices, processing speed, reliability and cost advantages and characteris- tics. Particularly the significant challenges and competitiveness of Direct Digital Manufacturing technology in the processing of any complexity created directly the number of objects, internal structure and channel function, as well as the shape of the chassis components and structure of the matching and optimization.     

Circulating Monocyte Subsets and Transcatheter Aortic Valve Replacement

Transcatheter aortic valve replacement (TAVR), as an alternative to open heart surgery, has revolutionized the treatment of severe aortic valve stenosis (AVS), the most common valvular disorder in the elderly. AVS is now considered a form of atherosclerosis and, like the latter, partly of inflammatory origin. Patients with high-grade AVS have a highly disturbed blood flow associated with high levels of shear stress. The immediate reopening of the valve during TAVR leads to a sudden restoration of a normal blood flow hemodynamic. Despite its good prognosis for patients, TAVR remains associated with bleeding or thrombotic postprocedural complications, involving mechanisms that are still poorly understood. Many studies report the close link between blood coagulation and inflammation, termed thromboinflammation, including monocytes as a major actor. The TAVR procedure represents a unique opportunity to study the influence of shear stress on human monocytes, key mediators of inflammation and hemostasis processes. The purpose of this study was to conduct a review of the literature to provide a comprehensive overview of the impact of TAVR on monocyte phenotype and subset repartition and the association of these parameters with the clinical outcomes of patients with severe AVS who underwent TAVR.     

基础振动下直动式减压阀动态特性分析

针对基础振动对TBM液压元件性能的影响,为TBM液压元件优化设计提供理论依据,选择直动式减压阀为研究对象,分析其工作原理,建立减压阀的动态响应数学模型,仿真研究基础振动幅值和频率对减压阀波动特性的影响规律,分析减压阀不同结构参数对压力波动的影响.结果表明：基础振动会引起减压阀出口压力波动,波动幅值随振动幅值增加而增大;当基础振动频率大于50 Hz时,压力波动幅值随频率的增加明显增大;减小背压腔初始容积和回流通道直径能提高减压阀的动态稳定性.     

仪器化冲击在评测断口剪切面积比例中的应用

根据示波曲线定量给出的特征载荷点分布关系，结合ESIS给出的评测夏比V型冲击断口剪切面积比例(PSF)经验公式，介绍了仪器化冲击试验在估算PSF数值中的具体应用。估算值与实际测量值所进行的误差离散性与置信区间的分析结果表明，选用合适的经验算法可以在试验过程中实时获得高度可靠的PSF结果。与常规光学手工评测技术相比，该方法不依赖于试验者的主观评测经验、不受特定客观试验条件的限制，具有广阔的应用前景，同时也为材料PSF评测以及动态断裂机理的研究提供了一套方便而有效的试验手段。     

天广直流输电系统过负荷功能研究

直流输电系统的快速响应和过负荷能力有利于提高整个电网的稳定性,基于天广直流输电系统的控制策略,介绍了其过负荷功能的计算和实现方法,并结合实例进行说明,最后还讨论了运行中应引起重视的相关问题。     

基于ST7单片机的太阳能直流供电系统控制器设计

设计一种用ST7单片机构成的全数字化太阳能直流供电系统控制器。系统在ST7单片机的控制下,能将太阳能电池转换的直流电进行有效存储和合理使用。系统充分利用了ST7单片机的软件资源,最大程度地简化了硬件电路,使电源具有较高的性价比和可靠性。     

基于间接矩阵变换器的异步电动机直接转矩控制

间接矩阵变换器是一种直接变换型交流-交流电力变换装置,与交-直-交变换器和传统矩阵变换器相比,具有很多优良的特性。异步电动机直接转矩控制以其简洁明了的系统结构,优良的静、动态性能,获得转矩的高动态性能。为了在实现异步电动机的高动静态性能调速的同时,满足电网电能质量的要求,提出一种采用间接矩阵变换器驱动异步电动机的直接转矩控制系统调制策略。利用这种调制策略,在异步电动机高速运行的条件下,一方面实现异步电动机的高性能交流调速,另一方面使系统具有良好的输入特性,能够满足日益严格的电网电能质量的要求。数值仿真结果验证这种调制策略的可行性。     

十二脉动高压直流输电系统交流滤波器设计

在单极十二脉动高压直流输电系统PSCAD/EMTDC模型的基础上,仿真分析该系统未采取任何滤波装置时交流侧电流的谐波特点,依据谐波分析结果确定滤波方案,设计滤波器相关参数,并且运用MATLAB软件绘制滤波器的阻抗特性曲线,最后通过对比加装滤波器前后交流侧电流快速傅里叶变换分析结果,验证该滤波器设计方案的合理性.