利用原生质体融合技术构建梨酒酵母工程菌

以菌株酿酒酵母Saccharomyces cerevisiae YDJ05及产香酵母Issatchenkia orientalis YS03为融合亲本X和Y,通过原生质体融合技术构建适合梨酒酿造的酵母工程菌,研究结果表明:利用EMS诱变亲本菌株Y,得到了一株精氨酸营养缺陷型菌株,亲本菌株X和Y采用蜗牛酶液在35℃下处理100min,得到菌株X、Y的原生质体融合率分别为93.6%、94.2%,再生率分别为27.8%、31.6%。以加热的方式灭活亲本菌株X,灭活时间为14min。在以PEG为促融剂的条件下对两株菌进行原生质体融合,经过优选得到融合子DJ02,其产酒精率、产香率等指标最优,分别达到了9.87%、0.37g/L。      

在线液面连续监测在油水井动态分析中的应用

塔河油田主力油藏为碳酸盐岩缝洞型油藏,辖区油井分布广且井间距离大,前期主要是依靠人工井口测试获取液面数据;受到人员及监测仪器设备数量及运行情况的影响,测试工作量大、监测数据存在滞后,人工监测不能实时全面反映油井能量变化、机采井异常等情况。针对油田生产中动液面无法连续测量的问题,开展了液面远程在线监测,实现液面的连续监测和远程采集。通过液面远程在线连续监测,为油水井动态分析提供完整液面数据,可及时判断油井生产状况、调整优化机采工作制度,保证油井处于高效、安全的生产状态,达到节能降耗和油井效益最大化的目的。     

中国能源生产的累计㶲需求分析

以㶲理论为基础, 结合基于投入产出方法的清单计算模型, 计算了我国能源生产的累计㶲需求, 对不同能源产品的累计㶲需求进行了对比, 并分析了高次生产的㶲消耗在累计㶲需求中的比例.结果表明:供应1MJ的各能源产品, 电力的累计㶲需求最高, 为3.4MJ;其次为炼油产品、焦炭等其他二次能源, 为1.55~1.63MJ;初级化石能源最低, 为1.18~1.23 MJ.与基于流程的清单计算模型相比, 基于投入产出方法的清单计算模型更适用于分析能源系统.     

等离子体散射分析的改进型交替隐式时域方法

提出了一种改进的交替隐式时域有限差分(FDTD)方法,运用该方法分析了等离子体涂覆导体圆柱的散射特性,为等离子体隐身技术的运用提供了一定的理论指导.该方法在整个问题空间采用完整场形式的场量进行迭代计算,并运用中心差分格式离散极化电流密度辅助方程.仿真实验表明,提出的算法具有无条件稳定特性,其计算效率明显优于以往文献提出的交替隐式FDTD方法,在精度和效率上也稍高于普通的显式FDTD方法.     

裂壶藻补糖发酵及糖氮代谢对产油的影响

为了探索裂壶藻(Schizochytrium limacinum)突变株生长及产油过程中对碳源和氮源的需求,本文对发酵各阶段的碳源和氮源利用变化情况进行测定,并设定五个在不同时期的补糖实验组进行研究,分别观测各组各时期生物量、油脂产量。结果表明,前96 h是裂壶藻生长的重要时期,生物量大幅度增长,在发酵144~168 h时,油脂产量达到最大,各组经发酵7 d收获总的藻体,对生物量、油脂产量、DHA含量和DHA产量进行综合测定,结果表明,在发酵120 h时进行补糖为最佳时期,且油脂产量及DHA产量相比于对照组分别高出18.80%和22.44%。当氮源耗尽时补糖不仅可以促进油脂的产生,而且对DHA的形成也是有利的。      

Photoprocesses in Derivatives of 1,4- and 1,3-Diazadistyryldibenzenes

Photoprocesses in 1,4-diazadistyrylbenzene (1) and 1,3-diazadistyrylbenzene derivative (2) diperchlorates in MeCN were studied by absorption, luminescence, and kinetic laser spectroscopies. For compound 1, trans-cis-photoisomerization and intersystem crossing to a triplet state are observed. For compound 2, photoelectrocyclization is suggested. Quantum chemical calculations of diazadistyrylbenzene structures in the ground and excited states were carried out. The schemes for photoreactions were proposed.     

Sensing Magnetic Field and Intermolecular Interactions in Diamagnetic Solution Using Residual Dipolar Couplings of Zephycandidine

An unusual residual dipolar coupling of methylene protons was recorded in NMR spectra because aromatic zephycandidine has preferential orientation at the external magnetic field. The observed splitting contains contribution from the dipole–dipole D-coupling and the anisotropic component of J-coupling. Absolute values of the anisotropy of magnetic susceptibility |Δχ_ax| are larger for protic solvents because of the hydrogen-bonding compared to aprotic solvents for which polar and dispersion forces are more important. The energy barrier for the reorientation due to hydrogen-bonding is 1.22 kJ/mol in methanol-d_4, 0.85 kJ/mol in ethanol-d₆ and 0.87 kJ/mol in acetic acid-d₆. In dimethyl sulfoxide-d₆, 1.08 kJ/mol corresponds to the interaction of solvent lone pair electrons with π-electrons of zephycandidine. This energy barrier decreases for acetone-d₆ which has smaller electric dipole moment. In acetonitrile-d₃, there is no energy barrier which suggests solvent ordering around the solute due to the solvent-solvent interactions. The largest absolute values of the magnetic anisotropy are observed for aromatic benezene-d₆ and tolune-d₈ which have their own preferential orientation and enhance the order in the solution. The magnetic anisotropy of “isolated” zephycandidine, not hindered by intermolecular interaction could be estimated from the correlation between Δχ_ax and cohesion energy density.     

Influence of Different Salts on the G-Quadruplex Structure Formed from the Reversed Human Telomeric DNA Sequence

G-rich telomeric DNA plays a major role in the stabilization of chromosomes and can fold into a plethora of different G-quadruplex structures in the presence of mono- and divalent cations. The reversed human telomeric DNA sequence (5′-(GGG ATT)₄; RevHumTel) was previously shown to have interesting properties that can be exploited for chemical sensing and as a chemical switch in DNA nanotechnology. Here, we analyze the specific G-quadruplex structures formed by RevHumTel in the presence of K⁺, Na⁺, Mg²⁺ and Ca²⁺ cations using circular dichroism spectroscopy (CDS) and Förster resonance energy transfer (FRET) based on fluorescence lifetimes. CDS is able to reveal strand and loop orientations, whereas FRET gives information about the distances between the 5′-end and the 3′-end, and also, the number of G-quadruplex species formed. Based on this combined information we derived specific G-quadruplex structures formed from RevHumTel, i.e., a chair-type and a hybrid-type G-quadruplex structure formed in presence of K⁺, whereas Na⁺ induces the formation of up to three different G-quadruplexes (a basket-type, a propeller-type and a hybrid-type structure). In the presence of Mg²⁺ and Ca²⁺ two different parallel G-quadruplexes are formed (one of which is a propeller-type structure). This study will support the fundamental understanding of the G-quadruplex formation in different environments and a rational design of G-quadruplex-based applications in sensing and nanotechnology.     

Assessing the DNA Damaging Effectiveness of Ionizing Radiation Using Plasmid DNA

Plasmid DNA is useful for investigating the DNA damaging effects of ionizing radiation. In this study, we have explored the feasibility of plasmid DNA-based detectors to assess the DNA damaging effectiveness of two radiotherapy X-ray beam qualities after undergoing return shipment of ~8000 km between two institutions. The detectors consisted of 18 μL of pBR322 DNA enclosed with an aluminum seal in nine cylindrical cavities drilled into polycarbonate blocks. We shipped them to Toronto, Canada for irradiation with either 100 kVpor 6 MV X-ray beams to doses of 10, 20, and 30 Gy in triplicate before being shipped back to San Diego, USA. The Toronto return shipment also included non-irradiated controls and we kept a separate set of controls in San Diego. In San Diego, we quantified DNA single strand breaks (SSBs), double strand breaks (DSBs), and applied Nth and Fpg enzymes to quantify oxidized base damage. The rate of DSBs/Gy/plasmid was 2.8±0.7greater for the 100 kVp than the 6 MV irradiation. The 100 kVp irradiation also resulted in 5±2 times more DSBs/SSB than the 6 MV beam, demonstrating that the detector is sensitive enough to quantify relative DNA damage effectiveness, even after shipment over thousands of kilometers.