Gold nanoparticles-coated eggshell membrane with immobilized glucose oxidase for fabrication of glucose biosensor

This work reports the fabrication and application of a glucose biosensor based on the catalytic effect of gold nanoparticles (AuNPs) on enzymatic reaction for blood glucose determination. AuNPs were initially in situ synthesized on the surface of an eggshell membrane (ESM) which was subsequently immobilized with glucose oxidase (GO_x) to produce a GO_x-AuNPs/ESM. The GO_x-AuNPs/ESM was positioned on the surface of an oxygen electrode to form a GO_x-AuNPs/ESM glucose biosensor. The effects of pH, concentration of phosphate buffer solution and amount of GO_x on the response of the GO_x-AuNPs/ESM glucose biosensor were studied in detail. AuNPs on GO_x/ESM can improve the calibration sensitivity (30% higher than GO_x/ESM without AuNPs), stability (87.3% of its initial response to glucose after 10-week storage) and shortens the response time (<30 s) of the glucose biosensor. The linear working range for the GO_x-AuNPs/ESM glucose biosensor is 8.33 μM to 0.966 mM glucose with a detection limit of 3.50 μM (S/N = 3). The biosensor has been successfully applied to determine the glucose in human blood serum samples and the results compared well to a standard spectrophotometric method commonly used in hospitals. Our work demonstrates that the developed GO_x-AuNPs/ESM glucose biosensor has potential in biomedical analysis.     

Aptamer biosensor for label-free impedance spectroscopy detection of thrombin based on gold nanoparticles

This paper presents a simple electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-thrombin-aptamer as a molecular recognition element. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were self-assembled on the surface of a bare electrode by using 1,6-Hexanedithiol as a medium. To quantify the amount of thrombin, changes in the interfacial electron transfer resistance (R_et) of the aptasensor were monitored using the redox couple of an [Fe(CN)₆]^3−/4− probe. The plot of (Reti−Ret₀)/Ret₀ against the logarithm of thrombin concentration is linear with over the range from 0.1 nM to 30 nM with a detection limit of 0.013 nM. Meanwhile, the packing density of aptamers was determined by cyclic voltammetric (CV) studies of redox cations (e.g., [Ru(NH₃)₆]³⁺) which were electrostatically bound to the DNA phosphate backbones. The results indicate that the total amount of aptamer probes immobilized on the gold nanoparticle surface is sixfold higher than that on the bare electrode. The aptasensor also showed good selectivity for thrombin without being affected by the presence of other proteins.     

A sensitive hydrazine electrochemical sensor based on electrodeposition of gold nanoparticles on choline film modified glassy carbon electrode

A highly sensitive hydrazine sensor was developed based on the electrodeposition of gold nanoparticles onto the choline film modified glassy carbon electrode (GNPs/Ch/GCE). The electrochemical experiments showed that the GNPs/Ch film exhibited a distinctly higher activity for the electro-oxidation of hydrazine than GNPs with 3.4-fold enhancement of peak current. The kinetic parameters such as the electron transfer coefficient (α) and the rate of electron exchange (k) for the oxidation of hydrazine were determined. The diffusion coefficient (D) of hydrazine in solution was also calculated by chronoamperometry. The sensor exhibited two wide linear ranges of 5.0 × 10⁻⁷-5.0 × 10⁻⁴ and 5.0 × 10⁻⁴-9.3 × 10⁻³ M with the detection limit of 1.0 × 10⁻⁷ M (s/n = 3). The proposed electrode presented excellent operational and storage stability for the determination of hydrazine. Moreover, the sensor showed outstanding sensitivity, selectivity and reproducibility properties. All the results indicated a good potential application of this sensor in the detection of hydrazine.     

Gold nanowire array electrode for non-enzymatic voltammetric and amperometric glucose detection

The non-enzymatic voltammetric and amperometric detection of glucose using a gold nanowire array electrode is described. The voltammetric detection of glucose was performed by cyclic and differential-pulse voltammetry. The detection of glucose by partial and direct oxidation of glucose during the anodic and cathodic potential sweeps was shown in cyclic voltammetry. An unusual decrease in overpotential for partial oxidation of glucose on a Au NW array electrode was observed. A linear differential-pulse voltammetric response for partial oxidation of glucose was observed up to a glucose concentration of at least 20 mM with a sensitivity of 41.9 μA mM⁻¹ cm⁻² and detection limit below 30 μM (signal-to-noise ratio of 3) for glucose oxidation at low potentials, where the influence of possible intermediates can be avoided. The amperometric response was also linear up to a glucose concentration of 10 mM with a sensitivity of 309.0 μA mM⁻¹ cm⁻². The wide dynamic range and high sensitivity, selectivity and stability, as well as good biocompatibility of the Au NW electrode make it promising for the fabrication of non-enzymatic glucose sensors.     

烧结矿品质预报的研究

某钢厂第二烧结车间现有90m^2、105m^2的烧结机各一台,担负着2座1000m^3高炉的供矿任务。随着高炉工艺的日益发展以及节能增效的要求,高炉对烧结矿品质的要求越来高。我国生产的烧结矿与国外生产的烧结矿有很大差距,重要原因就在于烧结矿的品质指标波动性太大,这对高炉的炉况来说是很不利的。所以,烧结矿品质预报的研究是很有必要的。     

矿石微机检测控制系统

为了提高矿山生产的管理水平,微机被应用于坑口矿石检测控制.提出了一个矿石微机检测控制系统,实现矿石的自动检测.从微机、接口部件以及软件等方面,介绍了该系统的设计与实现.该系统已推广使用,取得很好的效果.     

露天磷矿数字配矿模型

露天磷矿选矿厂采用合理的配矿方案,对稳定入选磷矿的品位,增加选矿产率,降低生产成本有着重要的意义.为了提高入选磷矿石品位的均匀性和稳定性,提出了针对以入选磷矿品位为目标的从采矿场配矿到精矿仓配矿的整个生产环节的配矿方法.将采场内原矿、选矿厂原矿矿堆和选矿厂精矿仓均定义为矿堆,按照生产实际需求设定其属性.通过设定目标函数,实现使采矿场的开采原矿量最大,配矿方案总的运输距离最小,运输成本最低等目标.同时建立约束条件,使采矿场的开采原矿量要保证选矿厂正常生产,使每一矿堆的品位达到目标要求.最后,采集企业生产实际数据对模型加以验证,证明模型在指导露天磷矿山的生产过程中有重要的实际意义,配矿过程应成为矿山生产组织、管理的一个重要环节.     

磷矿堆场多目标优化配矿模型

以磷矿浮选场堆场配矿过程为研究对象,选取人选原矿品质指标及低品质矿石利用量为主要目标,增加资源量约束及决策变量非负约束,构建了堆场多目标优化配矿模型.同时应用Matlab优化工具箱中多目标规划解算函数,对算例及实际配矿模型求解验证,并与现场混配结果进行比较,配矿效果明显.研究结果表明:磷矿多目标优化配矿模型实现了磷矿堆场配矿工艺及过程的简单、直观、较完整描述,Matlab优化工具箱多目标规划相关函数,对求解磷矿堆场多目标优化配矿模型是简便、有效的.     

磷矿反浮选捕收剂的评价方法

磷矿反浮选捕收剂大多数是由天然脂肪酸制成的,种类繁多,性能各异.从市场快速、准确地选择磷矿反浮选捕收剂,对浮选实践和浮选研究十分重要.浮选捕收剂的评价有助于掌握捕收剂的浮选性能,了解其在浮选过程中的作用.浮选法是浮选捕收剂的传统评价方法,程序复杂,耗时耗力.提出了一种新的浮选研究方法:燃烧-吸附量评价法,通过燃烧无机的矿石样品来测定其表面吸附的有机捕收剂量,评价磷矿反浮选捕收剂的性能.通过对油酸、硬脂酸和软脂酸这些结构明确且浮选工作者熟悉的脂肪酸的浮选性能和在白云石上的吸附量,建立了一个评价模型,验证燃烧-吸附量评价法的可行性.     

高精度磁测在沉积型磁铁矿区的找矿效果

沉积型磁铁矿脉特点是矿脉宽度小,品位低,综合磁性不均匀。通过对已知矿体进行剖面测量,证明高精度磁测的有效性,对野外实测数据进行日变校正、正常场校正、高度校正、水平梯度校正,化极、向上延拓、求取垂向二阶导数,以及对实测磁异常剖面进行人机对话反演等处理获得良好的效果。本次高精度磁测工作查明了矿区内磁铁矿（化）体的空间分布范围及大致埋深情况,圈定了几个有价值的磁铁矿（化）体靶区,取得了良好的找矿效果。