RT-PCR法检测庚型肝炎病毒C(GBV-C)RNA

应用RT－PCR法在非甲～非戊型肝炎患者血清及单采浆站供血员血浆中检测到GBV－CRNA，扩增产物经序列测定进一步确证，在30例非甲～非戊型肝炎患者中阳性率为6．6％；在600例单采浆供血者血浆中阳性率为0．33％。提示GBV－C可能不是非甲～非戊型肝炎的主要致病因子，可能存在GBV－C健康携带者。     

催化剂烧炭再生模拟和分析

催化剂烧炭再生是非定态过程。本文建立了绝热反应器内催化剂烧炭模型,利用移动边界正交配置法求解。以结炭的BO2催化剂再生为工作反应,模拟预测与实验值吻合良好。模拟计算表明,用递升进口温度序列烧炭,使再生时间明显缩短。     

基于引力搜索算法的分数阶变异时序回归GSA-TSGM(1,1)模型

为了利用分数阶累加算子在灰色短期预测中的高效性能,首次将分数阶累加算子引入变异时序回归模型以期取得更高的预测精度。主要方法如下：首先取湖北省链子崖某监测点1978—1987年的十年数据作为训练集并使用引力搜索算法确定最佳分数阶累加阶数,而1988—1993年的六年数据作为验证集验证提出的模型;其次对比了经典灰色模型GM(1,1)、分数阶累加灰色模型、变异时序回归模型TSGM(1,1)三种灰色模型。结果如下：首先修正了陈西江等人变异时序回归模型仿真时出现的错误,其次表明了相比于其他的模型,基于引力搜索算法的分数阶累加时序回归模型在进行灰色长期预测中具有较高的预测精度。因此,通过分数阶累加算子提高了灰色理论中长期预测模型的精度,为灰色长期预测提供了指导。     

A vibrational study of the activation sequence of C–H and C–C bonds of isobutene and 1-butene on Mo(110) and (4 × 4)-C/Mo(110) surfaces

The thermal decomposition pathways of isobutene and 1-butene on both Mo(110) and 4 × 4-C/Mo(110) surfaces have been studied using high-resolution electron energy loss spectroscopy (HREELS) in order to highlight the substantially different activities of these two surfaces towards the cleavage of C–H and C–C bonds. On clean Mo(110), the CH₂ group of isobutene decomposes upon heating to 150 K, producing either a /-bonded isobutenylidene [(CH₃)₂CCH] species or a 1,1-di-/-bonded isobutenyl [(CH₃)₂CC] species. Upon further heating, extensive C–H bond scission occurs to form hydrocarbon fragments which do not contain CH₃ or CH₂ groups, but appear to have largely intact carbon skeletons. By contrast, isobutene is molecularly adsorbed on the carbide-modified surface at 150 K. Further heating produces isobutylidyne [(CH₃)₂HCC] by 300 K, which subsequently decomposes via C–C bond scission to generate surface methyl groups. The different activation sequence of the C–H and C–C bonds of isobutene on clean and carbide-modified Mo(110) surfaces is also qualitatively confirmed by comparative studies of 1-butene on the two surfaces.     

Enumerating maximal bicliques in bipartite graphs with favorable degree sequences

We propose an output-sensitive algorithm for the enumeration of all maximal bicliques in a bipartite graph, tailored to the case when the degree distribution in one partite set is very skewed. We accomplish a worst-case bound better than previously known general bounds if, e.g., the degree sequence follows a power law.     

The elusive role of the N-terminal extension of {beta}A3- and {beta}Al-crystallin

ß-Crystallins are structural lens proteins with aconserved two-domain structure and variable N- and C-terminalextensions. These extensions are assumed to be involved in quaternaryinteractions within the ß-crystallin oligomers orwith other lens proteins. Therefore, the production of ßA3-and ßAl-crystallin from the single ßA3/A1mRNA by dual translation initiation is of interest. These crystallinsare identical, except that ßAl has a much shorterN-terminal extension than ßA3. This rare mechanismhas been conserved for over 250 million years during the evolutionof the ßA3/A1 gene, suggesting that the generationof different N-terminal extensions confers a selective advantage.We therefore compared the stability and association behaviourof recombinant ßA3- and ßAl-crystallin.Both proteins are equally stable in urea- and pH-induced denaturationexperiments. Gel filtration and analytical ultracentrifugationestablished that ßA3 and ßA1 both form homodimers.In the water-soluble proteins of bovine lens, ßA3and ßA1 are present in the same molecular weight fractions,indicating that they oligomerize equally with other ß-crystallins.¹H-NMR spectroscopy showed that residues Met1 to Asn22 of theN-terminal extension of ßA3 have great flexibilityand are solvent exposed, excluding them from protein interactionsin the homodimer. These results indicate that the differentN-terminal extensions of ßA3 and ßA1 donot affect their homo- or heteromeric interactions.     

水处理程序控制系统改造

介绍水处理程序控制系统改造过程及可编程序控制器(PLC)和工控机在水处理系统的应用。     

Kinetics of hydrogen peroxide bleaching of ALCELL® derived pulp

In this study, a kinetic model of the final bleaching stage with hydrogen peroxide in a totally chlorine free (TCF) bleaching sequence for ALCELL® processed pulp was developed. The model was based on the rate of chromophore destruction characterized by the decrease in the light absorption coefficient of bleached pulp at 457 nm, C_K. Based on the fact that the chromophore destruction proceeds rapidly in an initial phase followed by a much slower reaction during which a “floor-level” chromophore concentration is approached asymptotically, we propose that the hydrogen peroxide stage of the ALCELL® derived pulp in the studied TCF sequence consists of two distinct phases. The initial phase is a very fast reaction. The rate equation of the second phase was determined as: which is valid in a pH range of 10.5 to 11.5 and a temperature range of 60 to 92.5°C.     

Disassembly sequence planning using a Simplified Teaching–Learning-Based Optimization algorithm

Disassembly Sequence Planning (DSP) is a challenging NP-hard combinatorial optimization problem. As a new and promising population-based evolutional algorithm, the Teaching–Learning-Based Optimization (TLBO) algorithm has been successfully applied to various research problems. However, TLBO is not capable or effective in DSP optimization problems with discrete solution spaces and complex disassembly precedence constraints. This paper presents a Simplified Teaching–Learning-Based Optimization (STLBO) algorithm for solving DSP problems effectively. The STLBO algorithm inherits the main idea of the teaching–learning-based evolutionary mechanism from the TLBO algorithm, while the realization method for the evolutionary mechanism and the adaptation methods for the algorithm parameters are different. Three new operators are developed and incorporated in the STLBO algorithm to ensure its applicability to DSP problems with complex disassembly precedence constraints: i.e., a Feasible Solution Generator (FSG) used to generate a feasible disassembly sequence, a Teaching Phase Operator (TPO) and a Learning Phase Operator (LPO) used to learn and evolve the solutions towards better ones by applying the method of precedence preservation crossover operation. Numerical experiments with case studies on waste product disassembly planning have been carried out to demonstrate the effectiveness of the designed operators and the results exhibited that the developed algorithm performs better than other relevant algorithms under a set of public benchmarks.     

矿山工程设计可靠性研究

本文讨论了矿山工程设计中地质储量、矿山工程建设、工艺系统、露天矿投资等的可靠性计算方法,据此可对矿山能否达到设计规模及预期效益作出评价,从而可采取相应对策。