CD59活性位点相关性基因突变的构建与表达

构建了野生型和突变型CD59重组质粒,建立了高效真核表达系统,探讨了W40位点的生物学活性。采用基因点突变技术使CD59W40基因位置缺失（突变1,M1）及C39W40K41→W39W40W41（突变2,M2）,重叠延伸PCR（overlap extension PCR）定点诱变扩增突变基因,重组入真核表达质粒pIRES,利用阳离子脂质体（Lipfectamine2000）将重组质粒转染中国仓鼠卵巢细胞（CHO）进行表达。酶切鉴定及序列测定证实成功构建了pIRES-MICD59、pIRES—M2CD59和pIRES-WTCD59,突变基因约500bp。G418筛选出了CHO转染细胞的稳定细胞克隆,免疫荧光、ELISA检测筛选MICD59、M2CD59和WTCD59蛋白高表达株,连续传代30代有高表达;补体溶细胞反应显示与野生型CD59相比,突变型M1CD59失去对补体的抑制功能,而M2CD59抗补体活性略增高。证实CD59的W40位点对其功能具有重要作用,封闭此位点可提高补体活性,有望用于肿瘤治疗。     

基于Gabor变换的地震资料高分辨率处理方法研究

日益复杂的勘探目标对地震资料的分辨率提出了更高的要求,常规的反褶积和反Q滤波等高分辨率处理方法往往需要精确的先验信息,同时忽略了低频成分的补偿,不能满足实际生产需要。文中采用基于时频变换的高分辨率处理方法,在Gabor时频变换的基础上,引入倒频谱。利用倒频谱的思想,在时频域对振幅谱进行对数改造,保持相位谱不变,同时采用平滑处理以补偿低频成分,联合实现高分辨率处理。将该算法应用到雷克子波、薄层、楔形体模型以及实际地震数据中,结果显示子波持续时间减小,低于调谐厚度的薄层得到识别,频带拓宽,分辨率得到了提高,验证了该方法的有效性。最后,探究了时窗宽度对处理结果的影响,结果表明,为了达到更好的高分辨率处理效果,Gabor变换的高斯窗宽度应不低于子波的持续时间。     

基于新的特征向量和Kalman的复杂体制雷达合批算法

通过引入一种在分选中常出现增批现象的复杂体制雷达,在对其脉冲描述字的特点分析上指出了现有合批处理算法无法有效对其合批处理的原因。提出适合该雷达样式的新的特征向量。基于这个特征向量给出了一种基于Kalman的合批处理算法。仿真验证了该算法的有效性。     

扩展NS2用于主动分层多播仿真

分析网络仿真器NS2[3]进行多播仿真的内部机制,论述多播协议PIM-DM的仿真实现.给出一种对NS2进行扩展的方法,通过增加主动分类器、主动代理、修改复制器使之能用于主动分层多播协议的仿真.该扩展具有如下特点:分层数据以及信令数据均通过主动数据包传输,不同层数据在同一组播组发送.     

基于可拓理论的某型火炮系统性能水平评估

火炮系统性能水平直接影响着炮兵战斗力的大小,采用可拓分析理论来解决火炮系统设计和装备选择使用过程中的效能评估;先建立广义的火炮系统性能水平物元模型,再结合某型火炮系统实例应用,方法简便实用,结果准确直观,为火炮系统性能水平评估提供了一种新的方法。     

水灾灾度定量综合评价模型及应用

用物元可拓方法和反映洪水灾害灾度的指标以及层次分析法相结合,建立了水灾灾度定量综合评价模型.利用该模型对山东地区的水灾进行了灾度定量综合评价,结果表明不仅提高了水灾的定量化水平和精确程度,而且使水灾灾度定量评价更加综合和深入.     

Application of infrared thermography and extension recognize method to intelligent fault diagnosis of distribution panels

Distribution panels are an important piece of equipment in power systems. Maintaining a loading panel and ensuring security are not easy because the power supply cannot be interrupted in high‐tech factories. Distribution panels need fully functional condition monitoring and fault diagnosis systems to prevent accidents and reduce maintenance costs. In this paper, a new scheme is proposed to design an auto‐diagnosis tool for electrical distribution panels using infrared thermography to build diagnostic features using the matter‐element model. The paper then presents an extension recognize method (ERM) based on extension theory to diagnose the abnormality element by using degrees of correlation between the tested pattern and the matter‐element models that have been built up. Test results show that the proposed method can not only recognize the main defect element of the tested panel but also detect useful information for future trends and multi‐defect analysis by the correlation indexes. This new approach merits more attention as a tool to be used in the defect recognition problems of distribution panels, especially in high‐tech factories in Taiwan. © 2015 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.     

Compression and extension yield of an anisotropically consolidated soil

A series of undrained and drained triaxial tests were conducted to investigate the yielding behavior of anisotropically consolidated reconstituted Shanghai clay under triaxial compression and extension loading conditions. The soil was consolidated with K of 0.5, where K is the ratio of cell pressure to axial pressure. The tests included drained constant η (=q/p′) loading tests and undrained and drained triaxial compression and extension shear tests on anisotropically consolidated specimens. It was found that the anisotropically consolidated Shanghai clay obeys Rendulic’s principle under compression loading, but not under extension loading, and that the yield surface inclined inside the state boundary surface under extension loading. Moreover, the pre-yield behavior and the post-yield behavior were found to be very different under these two loading conditions (compression and extension loading). The test results confirm the validity of the sloping elastic wall theory, and the influence of anisotropic parameter β on the shape of the yield curve in the p′?v plane was also studied in the paper. Constitutive modeling methods for anisotropic consolidated soils were also discussed based on the test results.     

数字图像相关法测定岩石Ⅰ-Ⅱ复合型裂纹应力强度因子

提出了一种通过数字图像相关方法测定岩石Ⅰ-Ⅱ复合型裂纹尖端位置和应力强度因子的试验方法。试验通过数字图像相关方法测定裂纹尖端区域位移场数据,将位移场数据带入极坐标系下位移场方程,计算裂纹尖端位置和应力强度因子。试验结果表明：采用该方法可以准确的测定岩石Ⅰ-Ⅱ复合型裂纹应力强度因子、裂纹尖端位置及裂纹扩展长度,解决了以往研究方法因不能准确测定裂纹尖端位置和扩展方向,而无法准确测定岩石应力强度因子的难题。     

Discussion of papers

This paper describes a bridge condition assessment model that is based upon the simplification of a computational technique called resolution identity of fuzzy sets. The proposed model utilizes a fuzzy weighted average to combine fuzzy bridge condition ratings. The procedure is based on the decomposition of fuzzy sets into non-fuzzy level-sets or intervals. The utility and effectiveness of this procedure are illustrated with an example of the bridge condition assessment problem.