Effects of brefeldin-A on Golgi morphology in human cultured fibroblasts observed in three-dimensional stereo scanning electron microscopy

Brefeldin A (BFA) has been reported to cause disassembly of the Golgi. We have used three-dimensional (3-D) high-resolution scanning electron microscopy (HRSEM) to investigate these effects in human skin fibroblast cells. The spontaneous reassembly during prolonged exposure to BFA and some effects of forskolin were observed. A BFA concentration of 5μg/ml caused Golgi complexes to become vesicular, resulting in a progressive decrease in the size of the Golgi. Morphologic changes were visible within 2 min of BFA incubation, and by 30 min no identifiable Golgi could be found. Spontaneous reassembly of the Golgi apparatus upon the removal of the BFA or with continued long-term exposure with BFA could not be confirmed. Preliminary experiments with forskolin were not effective in reversing or inhibiting the effects of BFA in human fibroblast cells grown in culture. This inability for spontaneous reassembly and nonreversal by forskolin may reflect a differential effect of BFA in various cell types. HRSEM has proven to be useful for observing 3-D morphologic effects of BFA in Golgi.     

Pit to crack transition in stress corrosion cracking of a steam turbine disc steel

Long term exposure tests have been carried out on a 3 NiCrMoV steam turbine disc steel in the form of cylindrical tensile test specimens self-loaded to 90% of σ_0.2 and exposed to three environmental conditions, viz. deaerated pure water, aerated pure water, and aerated water containing 1.5 ppm of chloride ion. Pitting occurred in all environments but the density and depth of pits in the chloride-containing medium was markedly greater. No cracking was observed in deaerated pure water but cracks initiated in aerated water between 13 and 19 months and in less than 7 months in aerated 1.5 ppm Cl⁻ solution. The probability of a crack initiating from a pit of specific depth in aerated solution could be described well by a Weibull function. Profiling of pits and cracks in the disc steel tested in aerated 1.5 ppm Cl⁻ solution showed that there while there were many cracks with a depth greater than that of the corresponding pit the depth of some cracks was smaller than that of the corresponding pit, suggesting that cracks do not necessarily initiate from the bottom of the pits. The growth rate of short cracks emerging from pits appeared greater than that of long cracks in fracture mechanics specimens.     

采空区治理工程中注浆管的起拔及重复利用

以某采空区治理工程为例，介绍了采用正反丝连接注浆管的新方法，成功地解决了注浆管的起拔及重复利用问题，并对新法与不拔管及常规拔管方法的经济性进行了分析比较。     

Analysis and visualisation with host-based software – The features of STNAnaVist™

This article is based on experiences with data and text mining to gain information for strategic business decisions, using host-based analysis and visualisation (A/V), primarily in the field of patents. The potential advantages of host-based A/V are pointed out and the features of the first such A/V software, STN^®AnaVist™, are described in detail. Areas covered include the user interfaces, initial set of documents for A/V, data mining, text mining, reporting, and suggestions for further development.     

大电流监测信号提取的光收发及信号处理

近年来,大电流的监测成了人们十分关心的问题,这是由于大电流有强磁、高压、电流大等一系列的直接监测所不能克服的问题。这就需要把高压大电流的强电信号,经过提取后变成能在实验室中直接观测的弱电信号。为此,如何从高压大电流的信号中提取出随实时信号变化而实时变化的小信号就变成了一个关键问题。详细介绍了监测信号提取的光收发电路原理和后续信号处理电路,分析了各部分的作用,给出了必要的分析结果。     

串行接口LED数码管及键盘管理器件ZLG7289A的原理与应用

介绍了LED数码管及键盘管理器ZLG7289A的原理与应用。该芯片具有SPI串行接口，可同时驱动8位共阴式数码管(或64只独立LED)，还可连接多达64键的键盘矩阵，单块芯片即可完成LED显示、键盘接口等全部功能。文章给出了ZLG7289A的特点、引脚功能、指令说明和经典电路．并以单片机AT89C51为例，给出了其接口电路及相应源程序。     

数据挖掘技术在大坝安全决策支持系统中的应用

数据挖掘技术是一个新兴的，具有广阔应用前景和极富挑战性的研究领域。在充分研究大坝安全决策支持系统的基础上，结合数据挖掘技术的概念与方法等，对数据挖掘技术在大坝安全决策支持系统中的应用进行论述，与此同时，运用数据挖掘技术分析了某大坝垂直位移测值突变的物理成因。     

二维地震过障碍观测系统模式及其参数设计

在二维地震勘探中,为了避免因地表障碍物使地震反射剖面出现间断现象,需要改变观测系统设计,跨越障碍物(江河、城镇等),以保证反射同相轴能连续追踪对比。在以往地震勘探中,有时因过障碍观测系统设计不合理,不但增加勘探费用,而且影响采集质量。因此只有科学合理地设计过障碍观测系统模式和参数,才能保证地震测线顺利通过障碍物,得到较好的障碍物下方的地震资料并降低勘探成本。为此本文在调查和研究大量野外实际的过障碍观测系统的基础上,总结归纳成三大类过障碍观测系统模式,并分析其特点和应用范围。采用图解法,推导出主要模式的跨越宽度、最小炮检距及最大炮检距等参数公式。采用这套过障碍观测模式不仅可以得到和障碍区两侧等质量的地震资料,而且可以有效地降低勘探成本。