STUDIES ON COUNTERACTING CHROMATOGRAPHIC ELECTROPHORESIS AND ITS UTILIZATION FOR PROTEIN PURIFICATION

A counteracting chromatographic electrophoresis(CACE)separation device has been success-fully built and tested.Experiments are performed with protein mixtures of BSA,Hg,Mg and Cyto-cto study the separation performances,concentration profiles,start-up methodology as well as influ-ences of pH and ionic strength on the separation process.Mathematical models based on the fluiddynamics of the porous medium are proposed.Experiments indicate that the CACE process has agood commercial potential in protein engineering and fine chemicals purification.     

悬浮法合成固体粒状丙烯酸树脂

研究了悬浮法合成固体丙烯酸树脂颗粒。讨论了不同软硬单体配比对丙烯酸树脂颗粒的玻璃化转变温度、相对分子质量及其分布的影响以及不同的引发剂及悬浮剂对其聚合过程、最终产物形态及上述性能的影响。制得的丙烯酸树脂的数均相对分子质量用凝胶渗透色谱(GPC)测定为3.9×104～6.2×104,相对分子质量分布为2.04～2.32;用红外光谱图表征了制得的丙烯酸树脂结构。差示扫描量热法(DSC)测定了聚合物的玻璃化转变温度(Tg)。     

Enabling customers engagement and collaboration for small and medium-sized enterprises in ubiquitous multi-channel ecosystems

Over the last few years, we have encountered an exponential growth in online communication opportunities. Organizations have more and more ways to connect and engage with their current or future customers. The existence of more opportunities in connecting to people can be both an enabler and a burden. Being present at a multitude of different channels requires the effective management of a very large number of adapted contents, formats, and interaction patterns fulfilling the communication and cooperation needs of distributed target groups. In this respect, we integrate existing fragmented communication and monitoring approaches into a full-fledged communication model as a basis for an adequate engagement approach. We describe applications of our approach in both the eTourism and manufacturing domain. In this paper, we introduce an approach that will enable communication, collaboration and value exchange of users through a multitude of online interaction possibilities based on the use of semantic technology. Finally, we also compare our approach with existing solutions with respect to the identified challenges in this subject.     

基于MIKE Flood的中小河流溃堤洪水演进数值模拟

针对水文资料缺乏的中小河流,以贵溪市罗塘河下游段为例,应用MIKE软件对河流溃堤洪水演进进行了研究。采用瞬时单位线法推求断面各频率的设计洪水,并将其作为洪水演进模型的输入;利用MIKE软件建立了贵溪市罗塘河下游段的一、二维耦合水动力模型,对该河下游段溃堤洪水演进过程进行了模拟;通过模拟分析,得到了溃口流量过程和堤防保护区洪水淹没过程。研究成果可为中小河流洪水风险分析和灾害损失评估提供技术支撑。     

水中"一锅"合成7,7-二甲基-4-(3-氯苯基)-5-氧代-3,4,5,6,7,8-六氢香豆素的洁净方法

研究了四丁基溴化铵(TBAB)催化下的水介质中,3-氯苯甲醛、丙二酸亚异丙酯和5,5-二甲基-1,3-环己二酮三组分缩合,一步合成系列7,7-二甲基-4-(3-氯苯基)-5-氧代-3,4,5,6,7,8-六氢香豆素。产物的结构经IR和1HNMR进行了表征确证。该方法具有反应条件温和、操作简便、环境友好的优点。     

连续介质模型在边坡渗流分析及疏干工程中的应用

针对露天矿山高边坡地下水渗流场的特点，通过对等效连续介质渗流运动方程中水压力传导系数的剖析和现场疏于工程实践总结，提出了有实用价值的边坡水压流干工程设计方法。     

聚酯装置热媒系统串级使用节能改造

现有部分聚酯车间设计和建设时间较早,装置能耗较高,为了节能降耗,对现有热媒系统进行改造,一是将装置高温回油梯级使用到低温系统;二是改造部分热媒系统为串级使用,停开或降低热媒泵电耗。经过以上一系列改造和优化,有效提高了热能使用效率,减少了装置耗电,从而实现了热媒系统的节能降耗。     

重介洗煤密度自控系统研究

目的：本文介绍了PLC在洗煤厂的介质密度控制中的应用,针对介质密度变化规律性不强,大滞后等特点作出调整。方法：采用VB编程语言结合专家算法的方针,提出了一个解决方案,提高了控制密度精度。结果：使物料浓度配比更加合理,趋势变化更容易让现场人员接受。结论：提高了产煤精度及生产效率,节约了能源的同时为洗煤厂创造更大的经济回报。     

Cesium salt of a heteropolyacid in nanotubular channels and on the external surface of SBA-15 crystals: preparation and performance as acidic catalysts

The Cs-salt of heteropolyacid with stoichiometry Cs_2.5H_0.5PW₁₂O₄₀ (CsHPW) was deposited selectively at the external surface of the SBA-15 silica microcrystals, inside its mesoporous channels and simultaneously at both location modes. The structure, texture and performance of these CsHPW/SBA-15 composites were compared with that of a reference bulk salt of the same composition. Location of CsHPW salt on the external surface of SBA-15 microcrystals leads to disintegration of its agglomerates increasing acidity of the catalytic phase. A novel preparation strategy consisting of grafting the basic Cs-species at the internal pores surface of SBA-15 stabilized the CsHPW phase inside the channels in form of 5–8 nm nanocrystals at 30–70 wt.% loadings. The catalytic tests demonstrated that insertion of the CsHPW catalytic phase inside the nanotubular channels of SBA-15 in combination with location of an additional amount of this phase at the external surface of SBA-15 microcrystals allows to increase the specific activity of this phase in MTBE synthesis, propionylation of anisole and alkylation of catechol with t-butanol by a factor of 1.5–3. This level of specific activity in combination with high total loading of catalytic phase >60 wt.% permit to get composite catalytic materials with catalytic activity higher by a factor of 1.2–1.5 with respect to the bulk CsHPW catalyst and stabilizing the catalytic phase against colloidization in polar media.     

公共制造云服务平台架构及其支撑技术研究

为了能使量大面广的中小企业充分享受云制造的益处, 分析了公共制造云的运行原理, 搭建了一个面向中小企业的公共制造云服务平台架构, 并给出了其形式化描述。对平台所涉及的关键支撑技术如云制造资源管理、协同设计及QoS管理进行分析, 提出了基于多级递阶控制结构的云制造资源管理与监控模型, 以及在云制造基础设施之上构建了支持复杂产品设计、面向服务的虚拟协同设计环境。最后通过公共制造云服务平台运作模型说明了平台的应用性和示范性。