Group path covering and distance two labeling of graphs

For a positive integer d, an L(d,1)-labeling f of a graph G is an assignment of integers to the vertices of G such that |f(u)−f(v)|?d if uv∈E(G), and |f(u)−f(v)|?1 if u and u are at distance two. The span of an L(d,1)-labeling f of a graph is the absolute difference between the maximum and minimum integers used by f. The L(d,1)-labeling number of G, denoted by λd,1(G), is the minimum span over all L(d,1)-labelings of G. An L^′(d,1)-labeling of a graph G is an L(d,1)-labeling of G which assigns different labels to different vertices. Denote by the L^′(d,1)-labeling number of G. Georges et al. [Discrete Math. 135 (1994) 103-111] established relationship between the L(2,1)-labeling number of a graph G and the path covering number of Gc, the complement of G. In this paper we first generalize the concept of the path covering of a graph to the t-group path covering. Then we establish the relationship between the L^′(d,1)-labeling number of a graph G and the (d−1)-group path covering number of Gc. Using this result, we prove that and for bipartite graphs G can be computed in polynomial time.     

Necessary optimality conditions for fuzzy variational problems

The main aims of this study are to derive the fuzzy Euler-Lagrange conditions for both fuzzy unconstrained and constrained variational problems based on the concepts of differentiability and integrability of a fuzzy mapping that may be parameterized by the left and right-hand functions of its α-level sets.     

Strong stability of the mono-tubular heat exchanger equation with output feedback

We investigate asymptotic behavior of the C₀-semigroup T(t) associated with the mono-tubular heat exchanger equation with output feedback by a perturbation method. It is shown that T(t) is bounded if a constraint is satisfied by the parameters and the spatial distribution function. Further, applying the Arendt-Batty-Lyubich-Vu theorem, a criterion is established to judge strong stability of T(t).     

Numerical inversion of multidimensional Laplace transforms by the Laguerre method

Numerical transform inversion can be useful to solve stochastic models arising in the performance evaluation of telecommunications and computer systems. We contribute to this technique in this paper by extending our recently developed variant of the Laguerre method for numerically inverting Laplace transforms to multidimensional Laplace transforms. An important application of multidimensional inversion is to calculate time-dependent performance measures of stochastic systems. Key features of our new algorithm are: (1) an efficient FFT-based extension of our previously developed variant of the Fourierseries method to calculate the coefficients of the multidimensional Laguerre generating function, and (2) systematic methods for scaling to accelerate convergence of infinite series, using Wynn's ε-algorithm and exploiting geometric decay rates of Laguerre coefficients. These features greatly speed up the algorithm while controlling errors. We illustrate the effectiveness of our algorithm through numerical examples. For many problems, hundreds of function evaluations can be computed in just a few seconds.     

表单提交对象化-Struts

在b／s结构中可以完全实现逻辑和界面的分离，而它们之问的数据交流只有一个途径：表单提交。实现表单提交的对象化一直是很多程序员的愿甲，水文将通过对struts的介绍末展示其恭本工作原理，阐述如何用struts实现表单提交对象化：     

TGFβ-Treated Placenta-Derived Mesenchymal Stem Cells Selectively Promote Anti-Adipogenesis in Thyroid-Associated Ophthalmopathy

Orbital fibroblasts (OFs) in thyroid-associated ophthalmopathy (TAO) are differentiated from pre-adipocytes and mature adipocytes; increased lipid and fat expansion are the major characteristics of ophthalmic manifestations. Human placental mesenchymal stem cells (hPMSCs) were reported to immunomodulate pathogenesis and suppress adipogenesis in TAO OFs. Here, we prepared transforming growth factor β (TGFβ, 20 ng/mL)-treated hPMSCs (TGFβ-hPMSCs) in order to enhance anti-adipogenic effects in vitro and in TAO mice. TAO OFs were grown in a differentiation medium and then co-cultured with hPMSCs or TGFβ-hPMSCs. TAO OFs were analyzed via quantitative real-time polymerase chain reaction, Oil red O staining, and western blotting. The results showed that TGFβ-hPMSCs reduced the expression of adipogenic, lipogenic, and fibrotic genes better than hPMSCs in TAO OFs. Moreover, the adipose area decreased more in TAO mice injected with TGFβ-hPMSCs compared to those injected with hPMSCs or a steroid. Further, TGFβ-hPMSCs inhibited inflammation as effectively as a steroid. In conclusion, TGFβ-hPMSCs suppressed adipogenesis and lipogenesis in vitro and in TAO mice, and the effects were mediated by the SMAD 2/3 pathways. Furthermore, TGFβ-hPMSCs exhibited anti-inflammatory and anti-fibrotic functions, which suggests that they could be a new and safe method to promote the anti-adipogenic function of hPMSCs to treat TAO patients.     

Insights into the Pharmacokinetics and In Vitro Cell-Based Studies of the Imidazoline I2 Receptor Ligand B06

The impact of neurodegenerative diseases (ND) is becoming unbearable for humankind due to their vast prevalence and the lack of efficacious treatments. In this scenario, we focused on imidazoline I₂ receptors (I₂-IR) that are widely distributed in the brain and are altered in patients with brain disorders. We took the challenge of modulating I₂-IR by developing structurally new molecules, in particular, a family of bicyclic α-iminophosphonates, endowed with high affinity and selectivity to these receptors. Treatment of two murine models, one for age-related cognitive decline and the other for Alzheimer’s disease (AD), with representative compound B06 ameliorated their cognitive impairment and improved their behavioural condition. Furthermore, B06 revealed beneficial in vitro ADME-Tox properties. The pharmacokinetics (PK) and metabolic profile are reported to de-risk B06 for progressing in the preclinical development. To further characterize the pharmacological properties of B06, we assessed its neuroprotective properties and beneficial effect in an in vitro model of Parkinson’s disease (PD). B06 rescued the human dopaminergic cell line SH-SY5Y from death after treatment with 6-hydroxydopamine (6-OHDA) and showed a crucial anti-inflammatory effect in a cellular model of neuroinflammation. This research reveals B06 as a putative candidate for advancing in the difficult path of drug discovery and supports the modulation of I₂-IR as a fresh approach for the therapy of ND.     

Decreased Expression of Plakophilin-2 and αT-Catenin in Arrhythmogenic Right Ventricular Cardiomyopathy: Potential Markers for Diagnosis

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a hereditary disease of the heart muscle. Clinical challenges remain, however, in identifying patients with ARVC in the early or concealed stages with subtle clinical manifestations. Therefore, we wanted to identify potential targets by immunohistochemical (IHC) analysis in comparison with controls. Pathogenic mutations were identified in 11 of 37 autopsied patients with ARVC. As observed from IHC analysis of the RV, expression of αT-catenin and plakophilin-2 is significantly decreased in autopsied patients with ARVC as compared to controls, and the decreased expression is consistent in patients with and without pathogenic mutations. Furthermore, ARVC specimens demonstrated a reduced localization of αT-catenin, desmocollin-2, desmoglein-2, desmoplakin, and plakophilin-2 on intercalated discs. These findings have been validated by comparing RV specimens obtained via endomyocardial biopsy between patients with ARVC and those without. The pathogenic mutation was present in 3 of 5 clinical patients with ARVC. In HL-1 myocytes, siRNA was used to knockdown CTNNA3, and western blotting analysis demonstrated that the decline in αT-catenin expression was accompanied by a significant decline in the expression of plakophilin-2. The aforementioned effect was directed towards protein degradation rather than mRNA stability. Plakophilin-2 expression decreases concurrently with the decline in CTNNA3 expression. Therefore, the expression of αT-catenin and plakophilin-2 could be potential surrogates for the diagnosis of ARVC.     

Secreted HSP90α-LRP1 Signaling Promotes Tumor Metastasis and Chemoresistance in Pancreatic Cancer

The extracellular heat shock protein 90α (eHSP90α) has been reported to promote cancer cell motility. However, whether pancreatic cancer (PC) cells expressed membrane-bound or secreted HSP90α, as well as its underlying mechanism for PC progression, were still unclear. Our study demonstrated that the amounts of secreted HSP90α proteins were discrepant in multiple PC cells. In addition, highly invasive Capan-2 cells have a higher level of secreted HSP90α compared with those of less invasive PL45 cells. The conditioned medium of Capan-2 cells or recombinant HSP90α treatment stimulated the migration and invasion of PC cells, which could be prevented with a neutralizing anti-HSP90α antibody. Furthermore, secreted HSP90α promoted elements of epithelial–mesenchymal transition in PL45 cells, including increases in vimentin and Snail expressions, decreases in E-cadherin expression, and changes in cell shape towards a mesenchymal phenotype, but these phenomena were reversed by the anti-HSP90α antibody in Capan-2 cells. In addition, high levels of low-density lipoprotein receptor-related protein 1 (LRP1) were associated with worsened patient survival in pancreatic adenocarcinoma. We demonstrated LRP1 as a receptor of eHSP90α for its stimulatory role in metastasis, by activating the AKT pathway. In addition, silencing LRP1 enhanced the chemosensitivity to gemcitabine and doxorubicin in Capan-2 cells. Therefore, our study indicated that blocking secreted HSP90α underlies an aspect of metastasis and chemoresistance in PC.