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31.
Pomes R.; Willson R.C.; McCammon J.A. 《Protein engineering, design & selection : PEDS》1995,8(7):663-675
Free energy simulations are reported for the N31L-D mutation,both in the HyHEL-10-HEL antibody-lysozyme complex and in theunliganded antibody, using the thermo-dynamic-cycle perturbationmethod. The present study suggests that the mutation would changethe free energy of binding of the complex by 5.6 kcal/mol(unrestrained free energy simulations), by 0.5 kcal/mol(free energy simulations with a restrained backbone) and by1.8 kcal/ mol (Poisson-Boltzmann calculations, which also usea restrained geometry model). A detailed structural analysishelps in estimating the contributions from various residuesand regions of the system. Enhanced recognition of HEL by themutant HyHEL-10 would arise from the combination of thermodynamicallymore favorable conformational changes of the CDR loops uponassociation and subsequent charge pairing with Lys96 in theantigen. 相似文献
32.
Ir.H.E.M. Stassen 《Renewable Energy》1994,5(5-8)
Within the European Community, the interest in energy generation from biomass and waste is increasing. A brief overview is given of several thermal conversion technologies, of biomass production and waste availability in the Netherlands. Based on this, the possibilities for energy generation from biomass and waste in the Netherlands are evaluated. 相似文献
33.
PZT铁电薄膜的制备及电学性能研究 总被引:6,自引:1,他引:5
使用硝酸锆作锆源的溶胶-凝胶方法制备PZT铁电薄膜。他们的电学性能被测试包括电滞回线,电流I(t)特性,漏电流,矫顽场疲劳和老化。测到的PZT薄膜剩余极化8.2μC/cm~2,矫顽场68kV/cm.簿膜的寿命可超过10~(11)周期。 相似文献
34.
本文主要论述了开关磁阻电机传动技术的工作原理,系统结构和技术性能。介绍了该技术的发展背景、目前的应用情况和国内外发展水平。并对其前景表明了一些看法。 相似文献
35.
The influence of irreversibility of finite-rate heat transfer on the performance of an absorption refrigerator is investigated using an endoreversible cycle model with continuous flow. The cooling rate is adopted as an objective function for refrigerator optimization. The maximum cooling rate and the corresponding coefficient of performance are derived. The optimal performance with respect to heat transfer areas of the refrigerator is analysed. In addition, certain significant conclusions are reached. 相似文献
36.
稀疏效应下一类捕食者与食饵系统极限环 总被引:1,自引:0,他引:1
蔡淑云 《吉林化工学院学报》2002,19(4):95-97
研讨了稀疏效应下一类捕食者与食饵系统 :x· =x(x(r-x) -y) ,y· =b0 y(x -m) .讨论了该系统平衡点的性态 ,并证明了该系统极限环的存在性和唯一性 . 相似文献
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39.
J G De Nobel F M Klis A Ram H Van Unen J Priem T Munnik H Van Den Ende 《Yeast (Chichester, England)》1991,7(6):589-598
To study cell-cycle-related variations in wall permeability of Saccharomyces cerevisiae, two approaches were used. First, an asynchronous culture was fractionated by centrifugal elutriation into subpopulations containing cells of increasing size. The subpopulations represented different stages of the cell cycle as judged by light microscopy. Cell wall porosity increased when these subpopulations became enriched with budded cells. Secondly, synchronous cultures were obtained by releasing MATa cells from alpha-factor induced G1-arrest. These cultures grew synchronously for at least two generations. The cell wall porosity increased sharply in these cultures, shortly before buds became visible and was maximal during the initial stages of bud growth. It decreased in cells which had completed nuclear migration and before abscission of the bud had occurred. The porosity reached its lowest value during abscission and in unbudded cells. We examined the incorporation of mannoproteins into the wall during the cell cycle. SDS-extractable mannoproteins were incorporated continuously. However, the incorporation of glucanase-extractable mannoproteins, which are known to affect cell wall porosity, showed cyclic oscillations and reached its maximum after nuclear migration. This coincided with a rapid decrease in cell wall porosity, indicating that glucanase-extractable mannoproteins might contribute to this decrease. 相似文献
40.