Enhanced Anti-Cancer Effects of Conditioned Medium from Hypoxic Human Adult Dermal Fibroblasts on Cervical Cancer Cells

Hypoxia regulates fibroblast function by changing intracellular signaling and secretion factors, that influence the states of nearby cells. In this work, we investigated how medium (CM) from human adult dermal fibroblasts (HDFs) cultured in normoxic and hypoxic conditions affected cervical cancer (HeLa) cells. The HeLa cells showed decreased cell viability, increased apoptosis, and cell cycle arrest in response to CM from hypoxic-cultured HDFs (H-CM) compared with CM from normoxic-cultured HDFs (N-CM). Among the proteins up-regulated (>2-fold) in H-CM compared with N-CM, lymphotoxin-beta receptor (LTBR) decreased the viability of HeLa cells. Among the intracellular proteins down-regulated (>2-fold) in HeLa cells treated with H-CM compared with N-CM, the most enriched biological process GO term and KEGG pathway were protein deubiquitination and hsa05166:HTLV-I infection, respectively. In the protein–protein interaction network of intracellular proteins with altered expression (>2-fold), 1 up-regulated (TNF) and 8 down-regulated (ESR1, MCL1, TBP, CD19, LCK, PCNA, CHEK1, and POLA1) hub proteins were defined. Among the down-regulated hub proteins, the most enriched biological process GO term and KEGG pathway were leading strand elongation and hsa05166:HTLV-I infection, respectively. This study reveals that H-CM had stronger anti-cancer effects on cervical cancer cells than N-CM and induced intracellular signaling patterns related to those enhanced anti-cancer effects.     

中国大中型气田天然气的运聚特征

中国重点盆地大中型气田天然气的运聚具有多种途径和方式,归纳起来具有如下特点:区域构造运动是造成天然气运移的决定性控制因素;流体势决定天然气运移的方向和聚集的场所。在断陷盆地中,天然气以垂向和阶梯状运移为主;在克拉通、前陆斜坡和大型坳陷盆地中,天然气以侧向运移为主,垂向运移也起重要作用。断裂和不整合面是天然气运移的主要通道;异常压力是高压盆地天然气运移的主要动力,地热对热盆天然气的运移具有一定的影响;盆地的上倾方向和古隆起是天然气运移的指向区。     

基于多目标混料设计的重介质旋流器混料成分配比

为了准确掌握重介质旋流器各混料成分之间的配比关系,明确不同配比对旋流器产出质量和产量的影响,实现分选效果的最优化.分析了现有旋流器优化和混料配比研究的不足,探讨了采用混料设计方法解决上述问题的可行性和局限性。通过设计混料试验,运行试验得到试验结果,建立混料成分与各响应变量的数学模型,分析模型的有效性,以各响应变量的同时最佳为综合约束条件进行响应优化,通过求解苛求函数复合合意度最高时的各参数值,确定各混料成分的最优配比,并通过T假设检验验证试验结果用于生产的有效性。结合某选煤厂旋流器生产运行情况,明确旋流器中块煤、悬浮液和煤泥各混料的水平要求,采用极端顶点法设计混料试验并运行试验,建立三因子与精煤灰分和产率两个响应变量之间的回归模型,分析两响应变量同时最佳条件下最优的混料配比,得到三混料成分配比依次为15.5%、59.8%、24.7%,依据上述配比进行试生产,并采用T检验验证试验结果是有效的,从而为选煤厂高效生产和精确控制提供有效的支撑,也为后期实现自动化控制提供了可能。     

中密度纤维板生产减排技术研究与应用

对中型中密度纤维板生产中造成环境污染的污染源、污染因子进行了分析研究,根据同类企业实际运行情况提出了治理途径和解决措施。     

Modeling radiative properties of nanoscale patterned wafers

Temperature nonuniformity in rapid thermal processing of wafers is a critical problem facing the semiconductor industry. One cause of the problem is the nonuniform absorption of thermal radiation in patterned wafers where the optical properties vary across the wafer surface. This paper presents a parametric study of the radiative properties of patterned wafers, considering the effect of temperature, wavelength, and polarization. The finite-difference time-domain (FDTD) method is employed to examine the effe...     

电网规划中常规中长期负荷预测方法的探讨

电力负荷的不断增长是电网发展的根据,正确地预测电力负荷,既是为了保证国民经济发展及人民生活对电力的需求,也是电网自身发展的需求。电力负荷预测工作是电网规划工作的重要组成部分,也是电网规划的基础工作。在掌握铜陵地区经济、电网历史实绩的前提下,探讨了在电网规划中运用中长期常规负荷预测的几种方法,得出铜陵地区中长期负荷预测结果,以便为铜陵地区电网发展提供与此相适应的负荷保证。     

中、重冰区直线杆塔静态不平衡张力分析

2008年1-2月期间,50年一遇的低温、持续的极端天气造成了湖南、江西、浙江大量的直线杆塔串倒[1][2].这主要是因为耐张段内的连续档内的档距、高差相差悬殊,一侧覆冰而另一侧少冰或无冰,直线杆塔两侧产生了沿线路方向的不平衡张力[3～8].不平衡张力计算通用三种不同的力学模型,利用MATLAB程序实例模拟分析导线档距...     

基于周期波动及其影响分析的电力市场中长期电价预测

电力市场中的中长期电价受众多不确定因素影响,很难用传统的经验统计方法建模预测。现有的预测方法一般从系统模拟角度出发进行预测,需要较多的系统信息资料,难度很大。本文采用经验模式分解将电价分解成多个相对独立的分量,详细分析不同的因素对于这些分量的影响,从不同的时间尺度分析电价的影响因素。根据分量的特点有针对性地选择时间序列和支持向量机建模预测。实例研究表明该方法适合于分析和预测复杂的中长期电价。     

多层结构半透明球形粒子的辐射特性

基于蒙特卡罗光线踪迹法研究了半透明多壳层球形粒子的辐射性质,并通过BHCOAT程序验证其精度。对比光线踪迹法,分析了有效介质理论(effective medium theory,EMT)的应用范围,并用光线踪迹法计算了多层球形粒子内光谱吸收分布。研究结果表明,蒙特卡罗光线踪迹法适合于计算粒子尺度参数 >30的多层球形粒子辐射吸收。层间折射率和消光系数偏差较小时,有效介质理论结合Mie理论可用来近似估算多层粒子的辐射特性。当层间光学常数偏差较大时,特别是对于精度要求较高的情况,EMT-Mie理论不再适用。层间光学常数不同导致光谱辐射吸收分布显著不均匀。     

Thermal Shrinkage Behavior of Metal–Organic Frameworks

Thermal treatment of metal–organic frameworks (MOFs) as a post‐treatment approach has grown in popularity and resulted in various MOF‐derived materials. However, the widely used extreme thermolytic conditions (usually above 500 °C) lead to degradation in the well‐defined MOFs intrinsic properties. This work demonstrates that MIL‐101 calcined at medium‐temperature range (200–280 °C) partially breaks the coordination bonds that can introduce more accessible active sites, exhibiting a 10‐fold increase in oxidation activity while retaining its intrinsic structure and porosity. Another fascinating feature of MIL‐101 calcined in this temperature range is their temperature‐dependent shrinkage behavior, which is also found in many other types of MOFs. Based on different shrinkage ratios of various MOFs, yolk–shell MOFs@MOFs structures can be constructed through nonsacrificial template method. Overall, the structural and morphological evolution process of MOFs treated in the medium‐temperature range can open new horizons to develop efficient MOFs catalysts and design complex structures.