固定化复合酵母生产黍米黄酒的新工艺

以黍米为原料 ,采用固定化复合酵母细胞技术发酵生产黄酒 ,确定了最佳工艺条件 :固定化复合酵母细胞接种配比为酿酒酵母∶产酯酵母 =3∶2 ;固定化复合酵母载体填充率 8% ;发酵温度2 0℃ ;发酵时间 96h ;起始 pH4 0。在该条件下发酵生产的黄酒 ,口感风味较好 ,在缩短了发酵时间的同时总氨基氮和总酯含量较传统工艺都有了较大的提高。     

磁性壳聚糖表面离子液体固定化及其对丙烯酰胺吸附性能研究

以Fe3O4作为磁性纳米粒子,悬浮交联法制备磁性壳聚糖微球;以戊二醛作为交联剂在磁性壳聚糖微球表面固定离子液体;利用紫外分光光度法研究磁性壳聚糖固定化离子液体对丙烯酰胺的吸附性能.吸附动力学实验表明：2 h吸附容量为1.45 mg/g,3 h吸附基本达到平衡.结果显示：制备的磁性壳聚糖固定化离子液体对丙烯酰胺具有良好的吸附性能.     

窖泥糟醅发酵过程微生物多态性特征

窖泥糟醅是发酵过程微生物栖息的生态环境,其中微生物种群随发酵过程的进行而不停地演替和代谢,具有明显的多态性。其微：生物种群的多态性主要表现为厌氧异氧菌、甲烷菌、己酸菌、乳酸菌、硫酸盐还原菌、硝酸盐还原菌等。由微生物种群的多态性必然引起微生物种群作用特征的多态性、发酵代谢产物的多态性,必然也引起产酒质量的多态性。     

浓香型白酒生产中耐酒精酵母菌的选育研究

以宋河酒厂窖泥、酒糟、酒厂废水为样品,从中分离出100株菌株,经初筛、复筛和酒精发酵试验,最终获得1株耐酒精度为18%vol酵母菌,编号为A16。通过单因素实验确定最佳发酵条件为:发酵温度30℃、p H值4.5、碳源为葡萄糖、氮源为复合氮源(酵母膏和蛋白胨的比例为2∶1)、无机盐为Mg SO4·7H2O、接种量为10%、转速100 r/min、装液量为150 m L/250 m L;通过正交试验确定培养基最佳配比为:葡萄糖25%,酵母膏2%,蛋白胨1%,Mg SO4·7H2O 1%,(NH4)2SO41%,KH2PO41%,菌株A16的酒精得率70%。     

固定金属离子亲和膜分离技术：（Ⅱ）研究进展和应用前景

概述了亲和膜及其组件的研究现状以及固定金属离子亲和膜分离技术的研究进展指出了该技术的应用前景．     

Immobilized lipase reactors for modification of fats and oils—A review

This review focuses on the use of immobilized lipase technology to effect hydrolysis, ester synthesis, and interesterification reactions. The various immobilization procedures, reactor configurations, and process considerations are all reviewed and discussed.     

Synthesis of a new chitosan immobilized with β‐cyclodextrins and its adsorption properties for bilirubin

A new chitosan derivative of chitosan immobilized with β‐cyclodextrins (CTC) has been synthesized by the reaction of chitosan and allyl‐substituted β‐cyclodextrins (ASC). Its structure has been proved by Fourier transform infrared spectral analysis, X‐ray powder diffraction analysis, and chemical analysis. Its adsorption of bilirubin has been also studied. By the experiment, it has been found that this new product has good adsorption ability on bilirubin. At initial 2 h, the adsorption increased largely. The adsorption mechanism was also dealt with. It predicted that this new chitosan derivative could have application in removing the bilirubin. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 99: 1264–1268, 2006     

Adsorption of bismuth(III) by bayberry tannin immobilized on collagen fiber

The adsorption behavior of collagen fiber‐immobilized bayberry tannin towards Bi(III) at acidic pH values was investigated. The adsorption capacity of the adsorbent towards Bi(III) was 0.348 mmol g^?1 at 303 K, and increased with the rise in temperature. The adsorption isotherms of Bi(III) were in the shape of so‐called type II isotherms and could be described by an empirical equation, ln q_e = k + (1/n)C_e, which implies that chemical adsorption is predominant at lower concentrations of Bi(III) and that physical adsorption is involved at higher concentrations. The adsorption kinetics of Bi(III) on the immobilized bayberry tannin could be well described by the pseudo‐second‐order rate model, and the adsorption capacities calculated by the model were almost the same as those determined by actual measurements. The adsorbent could be regenerated by using 0.02 mol dm^?3 ethylenediaminetetraacetic acid (EDTA) solution after adsorption of Bi(III). The adsorption selectivity of the immobilized bayberry tannin towards Bi(III) in a Cu(II)–Bi(III) binary solution in acidic medium was remarkable. Therefore, it is strongly suggested that the immobilized bayberry tannin could be applied to the removal of Bi(III) from crude Cu(II) samples under proper conditions. Copyright © 2006 Society of Chemical Industry     

Recovery of uranium by tannin immobilized on matrices which have amino group

Tannin, which contains polyhydroxy groups, has a high affinity for uranium. Various tannin–protein complexes were prepared to develop new adsorbents for uranium recovery from seawater. Albumin tannate has a high ability to adsorb uranium from seawater. Tannin was immobilized on matrices which have multiple active amino groups, such as aminopolystyrene and poly(vinyl-4,6-diamino-s-triazine)—poly VT. Of these complexes, tannin immobilized on poly VT adsorbed uranium most efficiently from seawater and highly selectively from a solution containing various heavy metals; the uranium adsorption was very rapid and was pH dependent. This adsorbent therefore appears to have potential for use in a commercial process for uranium recovery from seawater or from uranium-containing waste water.     

氨基树脂固定化S-腺苷甲硫氨酸合成酶的研究

以氨基树脂为载体对S-腺苷甲硫氨酸(SAM)合成酶进行固定化,优化了酶的固定化条件并对固定化酶的性质进行了研究。优化的固定化条件为:戊二醛体积分数5%、SAM合成酶添加量20mg·g-1、固定化时间5h。所制备的固定化SAM合成酶的酶活力为476.8U·g-1,酶活力回收率为74.5%。与游离SAM合成酶相比,固定化SAM合成酶的稳定性大幅提高,在50℃孵育5h酶活力仍保留61.2%,而游离SAM合成酶则完全失活;在pH值为6.0~6.5、8.0~9.5的缓冲溶液中,固定化SAM合成酶也更加稳定;固定化SAM合成酶连续催化反应10批次,酶活力保留86.3%;固定化SAM合成酶在4℃储存30d,酶活力保留81.4%。固定化SAM合成酶米氏常数KATPm=0.14mmol·L-1,KLm-Met=0.28mmol·L-1。