银杏叶中肉桂酰黄酮糖苷的研究

采用柱层析和制备ＨＰＬＣ的方法,从银杏叶中有效地提纯出山奈素－３－０－鼠李糖（２″→１″′）葡萄糖（６″′→１″）肉桂酸酯和槲皮素－３－０－鼠李糖（２″→１″′）葡萄糖（６″′→１″）肉桂酸酯。用紫外、红外和１ＨＮＭＲ和１３ＣＮＭＲ对其结构进行了鉴定,分析了银杏叶和银杏叶提取物中上述两种肉桂酰黄酮糖苷的相对含量     

哒嗪酮类葡萄糖苷化合物的合成与表征

合成了一系列4,5-2H-1-取代6-羰基哒嗪酮3四乙酰葡萄糖苷类化合物,收率在58%～77%,均为右旋糖苷,其结构经IR与1HNMR表征。     

迎春花叶抑菌活性物研究

采用硅胶柱层析(CC)、薄层层析(TLC)和重结晶等技术,从迎春花叶乙酸乙酯萃取物中分离到一强抑菌活性单体。经理化检测和波谱分析数据鉴定属裂环烯醚萜葡糖苷类(secoiridoid glucosides)的迎春花素(jasminin)。该单体在强酸性环境中不稳定,高温、紫外光处理后抑菌活性稳定,对金黄色葡萄球菌的最小抑菌浓度(MIC)为均为1mg/ml,对痢疾杆菌、大肠杆菌的最小抑菌浓度为0.5mg/ml,对三个受试菌的最小杀菌浓度(MBC)为2mg/ml。     

罗汉果花中黄酮甙类化合物的抗氧化活性研究

采用FRAP、TEAC及ORAC三种体外抗氧化活性测定体系,分别测定了罗汉果花中5个黄酮甙类单体化合物的抗氧化活性,测定结果以μmol Trolox/g统一表示,并以此分析罗汉果花中黄酮甙类化合物的构效关系.结果表明,罗汉果花黄酮甙类化合物中,SF1-6-2和SF-3具有很好的抗氧化活性,原因是其结构中的7,3-位羟基是黄酮甙元的活性基团,是影响其抗氧化活性的关键基团,如果羟基糖甙化、羟基甲基化等都会使其抗氧化活性降低.     

车前子环烯醚萜苷类化合物与其抑制PTP1B的活性研究

蛋白酪氨酸磷酸酯酶1B在胰岛素信号传达过程中起负调控作用,也是研究治疗2型糖尿病的重要靶点.利用生物活性导向分离方法从车前子中分离得到6个环烯醚萜苷类活性化合物.通过光谱和文献比较鉴定,6种化合为Desacetylhookerioside（1）,Hookerioside（2）,Alpinoside（3）,Majoroside（4）,Anagalloside（5）和10-hydroxymajoroside（6）,其中化合物3和5可以有效抑制PTP1B的活性,其IC50值分别为（17.7±2.5）和（19.8±1.2）μM.     

The Distribution of Pyrimidinone Glucosides in Developing Seedlings of Vicia faba and Vicia narbonensis

The distribution of the pyrimidinone glucopyranosides vicine and convicine was investigated in resting seed and axenic developing seedlings up to 17 days of age in Vicia faba cv Troy, a low seed pyrimidinone glucopyranoside mutant (MTG5) derived from Troy, and a related species V narbonensis. The lower amounts of total vicine and convicine in resting seed of MTG5 were due primarily to a reduction in cotyledon pyrimidinone glucopyranosides, with the amount in the embryo axis much less affected. Distribution within developing seedlings revealed consistent patterns. A pulse of vicine appeared in both species in stems and roots at day 4 to day 7, then declined. Amounts of convicine rose initially then declined in seedling stems of both species, whereas in roots the rise in convicine continued throughout the period. The rise in convicine in the roots of V narbonensis could not have been accounted for by conversion from reserves of vicine. The low pyrimidinone glucopyranoside mutant MTG5 had less vicine initially in stems and roots than both Troy and V. narbonensis but by 17 days this had risen close to the levels of Troy and exceeded those of V narbonensis. By flowering, amounts of vicine and convicine in glass-house grown plants were similar in MTG5 and Troy. The levels of both compounds in the roots of MTG5 exceeded those of the sown seed. Consequently, both V faba and V narbonensis have the capacity to synthesise vicine and convicine in young plants. A mutation in a gene controlling levels of pyrimidinone glucopyranosides in the developing seed of V faba does not suppress the biosynthetic ability of young plants.     

烷基聚葡糖苷水溶液动态表面张力研究

采用最大泡压法研究了表面活性剂烷基聚葡糖苷（APG或CiGj）C8／10 G1.31和C12／14G1．43水溶液的动态表面张力（DST）;重点考察了胶束溶液中的DST曲线。将得到的13ST对时间的曲线,用Rosen模型进行处理,计算了DST的各项参数（n,ti、t^＊、tm、γm、γ0-γm、R1／2）。结果表明,随浓度增加,n、ti、t^＊、tm以及）γm减小,R1／2增大。当浓度大于cmc时,仍可用Rosen经验方程对数据进行处理,提出改进模型对胶束溶液中的动态吸附现象进行了解释。     

Therapeutic Approach of Flavonoid in Ameliorating Diabetic Cardiomyopathy by Targeting Mitochondrial-Induced Oxidative Stress

Diabetes cardiomyopathy is one of the key factors of mortality among diabetic patients around the globe. One of the prior contributors to the progression of diabetic cardiomyopathy is cardiac mitochondrial dysfunction. The cardiac mitochondrial dysfunction can induce oxidative stress in cardiomyocytes and was found to be the cause of majority of the heart morphological and dynamical changes in diabetic cardiomyopathy. To slow down the occurrence of diabetic cardiomyopathy, it is crucial to discover therapeutic agents that target mitochondrial-induced oxidative stress. Flavonoid is a plentiful phytochemical in plants that shows a wide range of biological actions against human diseases. Flavonoids have been extensively documented for their ability to protect the heart from diabetic cardiomyopathy. Flavonoids’ ability to alleviate diabetic cardiomyopathy is primarily attributed to their antioxidant properties. In this review, we present the mechanisms involved in flavonoid therapies in ameliorating mitochondrial-induced oxidative stress in diabetic cardiomyopathy.     

植物黄酮醇提取方法研究进展

黄酮醇是自然界中重要的天然色素及生物活性物质之一,在食品及保健品开发中发挥着重要作用。了解植物黄酮醇提取技术的最新研究进展对于有效获取植物黄酮醇并促进其在相关领域的应用具有重要意义。本文综述了近年来国内外有关植物黄酮醇提取的不同方法,对不同提取方法的工作原理及其优缺点进行了剖析,并对本领域后续发展方向进行展望,以期为植物生物活性物质分离提取及应用研究提供理论依据及技术支持。     

植物黄酮醇的检测方法研究进展

黄酮醇是一类与人体健康密切相关的生物活性因子,同时也是与植物源食品的色泽、口感和风味形成密切相关的次生代谢产物。定性定量分析对其相关产品的质量控制和安全性评价至关重要。但黄酮醇糖苷类化合物种类繁多、结构复杂,导致其检测分析十分困难。因此了解植物黄酮醇分析检测方法的研究进展,对于现有黄酮醇类化合物检测方法的完善及新型检测方法的开发具有重要意义。本文综述了国内外有关植物黄酮醇检测方法的最新研究进展,对不同检测方法的工作原理、优势和不足进行了分析,对领域现存问题进行定位并对后续研究重点进行展望,以期为植物类黄酮化合物的检测分析研究提供理论依据及技术支持。