低温等离子体处理模式对冷藏南美白对虾中假单胞菌的抑菌效果及机理研究

摘 要：目的 探究低温等离子体(cold plasma, CP)处理模式对冷藏南美白对虾中常见荧光假单胞菌(Pseudomonas fluorescens)的抑菌效果及其作用机制。方法 通过CP直接处理和循环处理P. fluorescens,研究了两种处理模式下臭氧含量动态变化对P. fluorescens的生长曲线、细胞活力,生物膜形成、细胞壁、细胞膜完整性和南美白对虾菌落总数及假单胞菌数等指标的影响。结果 两种处理模式在CP处理3 min或3 cycles后,包装内臭氧含量达到最高值,分别为(850±10) mg/m3和(874±20) mg/m3。CP循环处理模式使得臭氧含量随处理循环数递增,因此获得更长的臭氧存在时间从而具有更大的抑菌能力。P. fluorescens生长曲线表明CP处理使得菌体延迟期变长且对数生长期推迟。此外,CP处理后的P. fluorescens细胞活力显著下降(P<0.05),CP-1 min,CP-3 min和CP-3 cycles组的细胞活力分别为33.03%、5.90%和4.82%。同时相比CP-3 min组,CP-3 cycles组的P. fluorescens生物膜OD值下降27.61%。碱性磷酸酶(alkaline phosphatase, AKP)活性和核酸蛋白泄漏量结果表明,细胞壁和细胞膜完整性受损可能是P. fluorescens失活的直接原因。对虾保鲜测试结果证实,贮藏第6 d,CP-3 cycles组虾体中的菌落总数和假单胞菌数相比CP-3 min组分别降低了58.02%和79.54%。结论 CP循环处理模式通过延长对臭氧与对虾的暴露时间,提高了对P. fluorescens的灭活效果,同时还具有更优越的保鲜能力。本研究为开发基于CP技术的新型保鲜技术应用提供了理论参考。 关键词：低温等离子体;荧光假单胞菌;抑菌机制;保鲜     

Carbon nano-onion composites: Physicochemical characteristics and biological activity

Carbon nano-onion/surfactant (CNO/surfactant) composites offer the possibility to easily produce the soluble nanostructures. That approach combines the hydrophilicity of surfactants with the robustness of carbon structures to produce composites with superior and unusual physicochemical properties. We used the following surfactants: hexadecyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), sodium dodecyl benzene sulfonate (SDBS), 4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol (Triton X-100), and polyethylene glycol sorbitan monolaurate (Tween 20) to non-covalently modify CNO surfaces. The existence of stable CNO composites are clearly evidenced by direct transmission electron microscopy observations, which are also supported by thermogravimetric analyses. Dynamic light scattering and zeta potential confirmed their dispersion and stability. Additionally, the biological activity of well-dispersed CNO/surfactant composites against a strain of Escherichia coli was assayed. In vitro antimicrobial assays for the composites revealed that only the CNO/CTAB composite decreased cell viability. This activity could be assigned to the simple composite dissociation in water solutions, however antimicrobial properties of the composite are slightly better when compared with pure CTAB. This indicate some synergic effect with respect to the properties of the pure surfactant.     

Sorting and disposing of waste at recycling centres - A users perspective

This paper investigates Swedish recycling centres from the users' perspective. The aim was to describe the characteristics and experiences of the users and their activities when sorting and disposing of waste, and to identify improvements for the users. The typical recycling centre user is a recently retired man, living in a house with a garden, having travelled 5 km alone in his own car. The users requested longer opening hours and better information available at home and at the recycling centre. The major difficulty for the users is to understand which fraction their waste belongs to, and consequently into which container they should throw it. The most important sources of sorting information, in addition to experience from earlier visits, are signs and asking the personnel. Although the service at recycling centres is perceived positively by a majority of users, substantial improvements can still be made, and a number of such suggestions are given.     

Analysis of multi-agent activity using petri nets

This paper presents the use of place/transition petri nets (PNs) for the recognition and evaluation of complex multi-agent activities. The PNs were built automatically from the activity templates that are routinely used by experts to encode domain-specific knowledge. The PNs were built in such a way that they encoded the complex temporal relations between the individual activity actions. We extended the original PN formalism to handle the propagation of evidence using net tokens. The evaluation of the spatial and temporal properties of the actions was carried out using trajectory-based action detectors and probabilistic models of the action durations. The presented approach was evaluated using several examples of real basketball activities. The obtained experimental results suggest that this approach can be used to determine the type of activity that a team has performed as well as the stage at which the activity ended.     

Linux下多线程的视频图像平滑度评价算法

视频图像的平滑度评价算法是对视频质量评价的重要指标。平滑度与视频运动信息相关,具有计算量密集和实时性特点。随着流媒体业务的快速发展,传统的评价算法不能满足现状,为了对视频图像质量进行大规模处理,在现有的平滑度评价算法上,提出在Linux环境下处理视频图像,采用多线程技术实现该算法程序。试验结果证明:在Linux环境下实现的多线程算法比传统的串行算法提高了10倍的效率,在大规模计算密集型任务有具大的潜力。     

Error analysis of task performance with laptop in vibration environment

The present study investigates influences of vibration directions, vibration magnitudes, object sizes, object distances and angles of approach on producing errors while performing pointing and clicking activity on a laptop monitor similar to passengers working on moving trains. Attempts made outside the boundary of an object while performing the activity is registered as an error. The number of errors produced under different exposure conditions is computed. Higher vibration magnitudes, smaller size of the objects, and diagonal angular movements are found to create more errors and affected accuracy of the activity and hence showed difficulties to perform the activity.     

考虑用户活跃度和项目流行度的基于项目最近邻的协同过滤算法

项目相关性度量是基于项目最近邻的协同过滤算法的关键。已有的项目相关性度量方法在数据集稀疏或推荐低流行度产品时会面临较大挑战,因此提出一种考虑用户活跃度和项目流行度的基于项目最近邻的协同过滤算法。该算法在度量两个项目的相关性时,若有记录只对两个项目之一有评分,则利用该记录所对应的评分用户的活跃度和被评价项目的流行度进行相关性惩罚,从而提高数据稀疏环境下低流行度产品被推荐的概率。实验表明,所提算法在保证评分预测精度的情况下提升了推荐结果的多样性和新颖性。     

Tool choice in innovation diffusion: A human activity readiness theory

The short product life cycle of information and communication technology (ICT) applications makes it critical for developers to expedite the diffusion process by reducing user resistance. At the individual level, the adoption of an ICT application largely depends on how a person is ready to use the computerized tool in place of the corresponding traditional method for a certain purpose. To find out how to facilitate the transition, this study investigates the human choice behavior involved in technology adoption with the Activity Theory that allows for the inclusion of different tools in one unit of analysis. The understanding of user situated experiences from the activity perspective leads to hypothesized relationships between user-, tool- and task-related factors and the dependent variable in terms of tool readiness. The empirical results support that tool experiences have stronger effects on specific tool readiness at the within-subject level, user characteristics have weaker effects on general tool readiness at the between-subject level, and task situations influence both levels of tool readiness. The findings provide insights on how to facilitate innovation diffusion for different tools, tasks and users.     

A Novel Framework for Human Activity Recognition with Time Labelled Real Time Sensor Data

Human activity recognition is an effective approach for identifying the characteristics of historical data. In the past decades, different shallow classifiers and handcrafted features were used to identify the activities from the sensor data. These approaches are configured for offline processing and are not suitable for sequential data. This article proposes an adaptive framework for human activity recognition using a deep learning mechanism. This deep learning approach forms the deep belief network (DBN), which contains a visible layer and hidden layers. The processing of raw sensor data is performed by these layers and the activity is identified at the top most layers. The DBN is tested using the real time environment with the help of mobile devices that contain an accelerometer, a magnetometer, and a gyroscope. The results are analyzed with the metrics of precision, recall, and the F1-score. The results proved that the proposed method has a higher F1_score when compared to the existing approach.