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11.
12.
L. Thimon F. Peyoux R. Maget-Dana G. Michel 《Journal of the American Oil Chemists' Society》1992,69(1):92-93
The interfacial behavior of antifungal lipopetides of the iturinic group was studied in comparison with that of surfactin,
an anionic lipopeptide. All these lipopeptides were isolated from various strains ofBacillus subtilis; each strain produced surfactin and one antifungal compound. The iturinic compounds differ from surfactin by their lower
surfactant properties. The critical micelle concentration (CMC) values were dependent on the nature of the peptide moiety
in the iturinic compounds. The highest values were observed for anionic antibiotics. The arrangement of lipopeptides at the
air-water interface was largely dependent on the size of the lipid moiety; surfactin, which has a C14 or C15 β-hydroxy fatty acid, iturins A, C, and bacillomycins D, L, which have a C14 or C15 β-amino fatty acid, occupied a smaller area than mycosubtilin and bacillomycin F, which have a C16 or C17 β-amino fatty acid. These data can be related to bioactivity of these lipopeptides. 相似文献
13.
We identified many novel oxygenated FA produced from linoleic acid by microbial strain ALA2: 12,13,17-trihydroxy-9(Z)-octadecenoic
acid (12,13,17-THOA); 12,13,16-trihydroxy-9(Z)-octadecenoic acid (12,13,16-THOA); 12-hydroxy-13,16-epoxy-9(Z)-octadecenoic
acid; and 12,17;13,17-diepoxy-16-hydroxy-9(Z)-octadecenoic acid. 12,13,17-THOA, the main product, inhibits the growth of some
plant pathogenic fungi. Recently, we reclassified strain ALA2 as Bacillus megaterium ALA2 NRRL B-21660 and opened a possible link with the well-studied catalytically self-sufficient P450 monooxygenase of Bacillus megaterium ATCC 14581 (NRRL B-3712) and B. subtilis strain 168 (NRRL B-4219). Now we have found that strain ALA2 also oxidizes palmitic acid into three oxygenated products:
13-, 14-, and 15-hydroxy palmitic acids. This indicates that strain ALA2 also possesses a monooxygenase system similar to
the abovementioned well-known strains. These data facilitate studies on the oxygenase system of strain ALA2. 相似文献
14.
The potential of ozone for disinfection of ships’ ballast water was investigated using Bacillus subtilis spores as an indicator. The effects of pH, presence of iron, and bacterial strain on disinfection efficacy in seawater, under simulated ballast conditions, were investigated. Ozone dosages of 9 mg/L (pH 7) and 14 mg/L (pH 8.2) and 24 h contact achieved a 4-log inactivation with the various oxidant residuals formed. Iron surface at a ratio to water of 9 m2/m3 impaired the oxidant residuals and the disinfection of spores. Different strains of B. subtilis resulted in different CT values. Ozone does not seem to be a good choice for the control of spore-forming organisms in ballast water, but may be suitable for the control of other species. 相似文献
15.
纳豆制备过程中大豆异黄酮的转化条件 总被引:1,自引:1,他引:0
使用日本古法制备纳豆,从纳豆中分离筛选出高活性的纳豆菌为实验菌株。研究了纳豆制备过程中大豆异黄酮的转化情况,发酵液中的大豆异黄酮在大豆异黄酮糖苷酶的水解作用下水解掉侧链糖基,转化为活性更高的大豆异黄酮苷元。根据薄层层析法确定了大豆异黄酮转化为大豆异黄酮苷元的最佳培养条件:培养温度为37℃,培养时间2 d,pH 7.0,含水质量分数60%。发酵液中大部分的大豆异黄酮苷转化为大豆异黄酮苷元,从而得到更利于人体吸收的大豆异黄酮苷含量较高的纳豆制品。 相似文献
16.
The production and properties of the biosurfactant synthesized by Bacillus subtilis CCTCC AB93108 were studied. The maximum concentration of the surfactant is 1.64 g/L when the bacteria grow in a medium supplemented with glucose as carbon sources. The isolated biosurfactant is a complex of protein and polysaccharide without lipids. It reduces the surface tension of distilled water to 45.9 mN/m, and its critical micelle concentration (CMC) is 2.96 g/L. It can stabilize emulsions of several aromatic and aliphatic hydrocarbons, such as benzene, xylene, n-pentane, n-nonane, gasoline and diesel oil. It presents high emulsification activity and stability in a wide range of temperature (4-100℃) and a long period of duration. 相似文献
17.
一株枯草芽孢杆菌分离鉴定及其降解稠油特性 总被引:4,自引:1,他引:3
以稠油为唯一碳源,从被稠油污染过的土壤中筛选到一株高效石油烃降解茵,经生理生化鉴定和16S rDNA鉴定确认其为枯草芽孢杆茵.在摇瓶实验中,该菌最佳降解温度为35~45℃,最佳pH值为7.5~8.5,最佳盐质量浓度为8~16 g/L.在最佳降解条件下,当油质量浓度为0.1 g/L时,稠油降解率达34.3%.利用GC-nD分析知,该茵主要降解稠油中n-C9~n-C40的烷烃组分;利用GC-MS分析得知,该茵对蔡及烷基化萘去除彻底,对二苯并噻吩、芴和稠二萘等部分芳烃类化合物有降解作用,在稠油降解过程中菲及菲的衍生物有所增加. 相似文献
18.
利用Bacillus natto菌所产蛋白酶水解玉米醇溶蛋白,得到水解产物,采用凝胶层析技术,对水解产物的分离条件进行选择。结果表明,Sephadex G-25作为分离介质,流速为1.0 mL/min,上样体积为1.5 mL,在此条件下其分离效果较好,得到5个组分。 相似文献
19.
20.
Gerhard Gries John Clearwater Regine Gries Grigori Khaskin Skip King Paul Schaefer 《Journal of chemical ecology》1999,25(5):1091-1104
In 1996, the exotic white-spotted tussock moth (WSTM), Orgyia thyellina (Lepidoptera: Lymantriidae), was discovered in Auckland, New Zealand. Because establishment of WSTM would threaten New Zealand's orchard industry and international trade, eradication of WSTM with microbial insecticide was initiated. To monitor and complement eradication of WSTM by capture of male moths in pheromone-baited traps, pheromone components of female WSTM needed to be identified. Coupled gas chromatographic–electroantennographic detection analysis of pheromone gland extract revealed several compounds that elicited responses from male moth antennae. Mass spectra of the two most EAD-active compounds suggested, and comparative GC-MS of authentic standards confirmed, that they were (Z)-6-heneicosen-11-one (Z6–11-one) and (Z)-6-heneicosen-9-one, the latter termed here thyellinone. In field experiments in Japan, Z6–11-one plus thyellinone at a 100:5 ratio attracted WSTM males, whereas either ketone alone failed to attract a single male moth. Addition of further candidate pheromone components did not enhance attractiveness of the binary blend. Through the 1997–1998 summer, 45,000 commercial trap lures baited with 2000 g of Z6–11-one and 100 g of thyellinone were deployed in Auckland towards eradication of the residual WSTM population.Dedicated to my dear mother in honor of her 75th birthday 相似文献