Identification of durum wheat cultivars and monovarietal semolinas by analysis of DNA microsatellites

 Microsatellites isolated in bread wheat were used to identify 20 Italian durum wheat (Triticum turgidum var. durum L.) cultivars. A total of 15 microsatellite primer pairs were tested against DNA extracted from leaf tissue, single seeds and semolina. Twelve markers showed allelic polymorphism among the 20 cultivars, providing a total of 41 different alleles. Firstly, an analysis of microsatellite informativeness in the chosen set of durum wheat cultivars was made and a set of highly polymorphic microsatellites was established. Secondly, among the most polymorphic cultivars, the minimum number of microsatellites able to distinguish all cultivars was determined. A set of five microsatellites was found sufficient to differentiate the durum wheat cultivars examined. The method is directly applicable to seeds and semolina, and is suitable for detecting seed mixtures in the same seed lot. Received: 22 February 1999 / Revised version: 19 April 1999     

Microwave roasting and positional distribution of fatty acids of phospholipids in soybeans (Glycine max L.)

Whole soybeans were exposed to microwave roasting for 6, 12, and 20 min at a frequency of 2,450 MHz and were studied not only for phospholipid composition but also for positional distribution of the fatty acids. During microwave roasting, the greatest rate of phospholipid losses (P<0.05) was observed in phosphatidylethanolamine (PE), followed by phosphatidylcholine (PC) and phosphatidylinositol (PI), respectively. Therefore, the effects of microwave roasting on the composition and positional distribution of the fatty acids are likely clearer in PE than in PC or PI. However, the principal characteristics for the positional distribution of fatty acids are still retained during microwave roasting: unsaturated fatty acids, especially linoleic, are predominantly concentrated in the 2-position, and saturated fatty acids, especially palmitic, primarily occupy the 1-position after 12 or 20 min of roasting. The results suggest that unsaturated fatty acids located in the 2-position are significantly protected from microwave roasting.     

Changes in the processing quality of sugar beet (Beta vulgaris L.) during long-term storage under controlled conditions

Long-term storage of sugar beet is an option to extend the processing campaign of the sugar factories in Europe. In the present study, changes in the processing quality of sugar beet were quantified during 110 days of storage at 7 and 20 °C. During storage, the concentration of sucrose decreased whereas K, Na, amino N, betaine, invert sugar and raffinose accumulated in the beets, i.e. quality was impaired markedly. At 7 °C, the changes in sucrose concentration were relatively small but the formation of invert sugar and raffinose caused considerable losses in beet quality. Thus, long-term storage at low temperature is appropriate to prolong the campaign but processing costs will increase. Quality assessment with two formulae showed that measurements of K, Na and amino N are insufficient to evaluate stored sugar beets for recoverable sugar. Due to its increase in storage, the concentration of invert sugar should also be taken into account.     

Statistical analysis on Sicilian olive oils

A robust multivariate statistical procedure applied to an accurate gas chromatography (GC) analysis was used to analyze 1004 monovarietal and multivarietal Sicilian extra virgin olive oils coming from 22 cultivars of different geographical areas of Sicily and collected in nine years (1993, 1995, 1996, 1997, 1998, 1999, 2000, 2001 and 2002). The effect of the cultivar, of pedoclimatic conditions as well as of the year of harvesting on the olive oil fatty acid composition and therefore on their classification was investigated. Oleic, linoleic and palmitic fatty acids, important for the nutritional properties of an olive oil, showed a crucial rule in the characterization of olive oils.     

Effect of Ultra-Violet B on Release of Volatiles in Tea Leaf

Many volatiles in fresh tea leaf are in the forms bound with glycosides and they are released by action of ß–primeverosidase and ß-glucosidase during tea processing. Effect of ultra-violet B on release of volatiles in fresh tea leaves of two tea cultivars, “Maoxie” and “Anhui-9,” were investigated. Types of volatile and their concentration increased when ultra-violet B was applied at 3.0 μmol m^?2 s^?1 for 2 h and then decreased as the irradiation was extended to 6 h. The relation of expression of ß–primeverosidase and ß-glucosidase genes to the release of volatiles were also discussed.     

Genotypic and climatic influences on the concentration and composition of flavonoids in kale (Brassica oleracea var. sabellica)

The aim of this study was to determine the composition and concentration of flavonoid aglycones in kale, the dependence on genotype and their interaction with decreasing temperature and global radiation. Eight kale cultivars, comprising hybrid and traditional, old cultivars, were grown in a field experiment and harvested four times at 4-week intervals. The traditional, old cultivars in particular contained high concentrations of flavonoids. In all of the investigated cultivars, kaempferol was the main flavonoid aglycone, followed by quercetin and isorhamnetin, which was quantified in six of the eight cultivars. Furthermore, in six of the eight cultivars, the total concentration of flavonoids remained unchanged with decreasing temperature and global radiation. The quercetin concentration increased in five of these six cultivars, whereas the kaempferol concentration decreased. Interestingly, the quercetin-to-kaempferol ratio increased in all of the investigated cultivars, despite the fact that the radiation level decreased, suggesting that the impact of the decline in temperature could be responsible for this effect.     

Effect of cultivar and environment on physicochemical and cooking characteristics of field pea (Pisum sativum)

The effects of cultivar, growing location and year on physicochemical and cooking characteristics of field pea (Pisum sativum) were investigated and the relationship between these characteristics was determined. Six diverse field pea cultivars were grown in five different growing locations for two subsequent years (2006 and 2007). Cultivar, location and year had a significant effect on the seed weight, seed size, water hydration capacity, cooking time and firmness of cooked peas. Significant cultivar and location differences in protein, starch, crude fibre, fat, ash and phytic acid contents of peas were observed. Most of the traits were significantly affected by the interactions: cultivar-by-location, cultivar-by-year and location-by-year. Both seed weight and seed size were negatively correlated with crude protein and crude fibre contents. Cooking time was negatively correlated with crude fibre, ash and phytic acid contents, but positively correlated with firmness and starch content. A significant positive correlation was observed between ash content and phytic acid level in peas.     

2008～2010年外引甘蔗品种比较试验总结

为不断充实我州的甘蔗品种后备资源,我所在进行自育甘蔗新品种选育的同时,还从事外引甘蔗新品种的引进、试验、示范和推广工作。通过引种观察,从中筛选出了12个品种(系)进行品种比较试验,以期选出比本地当家品种丰产、高糖、宿根性好、适应性强、粗生、易管的优新良种在蔗区推广应用。     

Classification of South African red and white wines according to grape variety based on the non-coloured phenolic content

The phenolic content of South African wines was investigated for the purpose of their classification according to variety or cultivar. Twenty-two phenolic compounds were quantified by liquid chromatography in 55 red wines of five varieties and in 38 white wines of three varieties. Data were compared to literature values reported for wines from across the globe. ANOVA was used to determine significant concentration differences between the different varieties. Further grouping of the data was investigated by principal component analysis and cluster analysis. Finally, linear discriminant analysis was used to derive classification functions for the effective recognition of red and white wine varieties which allowed 100.0% and 97.4% correct recognition of red and white wines, respectively.     

南方主要鲜食葡萄品种的叶片形态指标鉴别研究

本文根据Galet的葡萄叶形结构测定原理,用多元判别和计算机图形分析,对我国南方主要栽培的4个鲜食葡萄品种(巨峰、藤稔、美人指、希姆劳特)的叶片形态参数进行数值化鉴别.结果表明,测量的7个叶形结构参数在区分4个鲜食葡萄品种上有不同的作用,同时根据叶片测量数据,绘制判别分布图和叶形结构参数均值脸谱图形,这有利于更好的对葡萄品种进行鉴别.