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51.
Siriluk Ratanabunyong Dr. Niran Aeksiri Dr. Saeko Yanaka Dr. Maho Yagi-Utsumi Dr. Koichi Kato Dr. Kiattawee Choowongkomon Dr. Supa Hannongbua 《Chembiochem : a European journal of chemical biology》2021,22(5):915-923
HIV-1 RT is a necessary enzyme for retroviral replication, which is the main target for antiviral therapy against AIDS. Effective anti-HIV-1 RT drugs are divided into two groups; nucleoside inhibitors (NRTI) and non-nucleoside inhibitors (NNRTI), which inhibit DNA polymerase. In this study, new DNA aptamers were isolated as anti-HIV-1 RT inhibitors. The selected DNA aptamer (WT62) presented with high affinity and inhibition against wild-type (WT) HIV-1 RT and gave a KD value of 75.10±0.29 nM and an IC50 value of 84.81±8.54 nM. Moreover, WT62 decreased the DNA polymerase function of K103 N/Y181 C double mutant (KY) HIV-1 RT by around 80 %. Furthermore, the ITC results showed that this aptamer has small binding enthalpies with both WT and KY HIV-1 RTs through which the complex might form a hydrophobic interaction or noncovalent bonding. The NMR result also suggested that the WT62 aptamer could bind with both WT and KY mutant HIV-1 RTs at the connection domain. 相似文献
52.
目的研究多表位DNA壳聚糖微球疫苗的体液免疫应答。方法制备多表位DNA壳聚糖微球疫苗pcD-NA3.1-HME-3C3d,经鼻腔免疫小鼠,蛋白检测微孔试剂盒检测小鼠特异性IgG抗体水平。结果经免疫的小鼠均能产生针对各表位的特异性IgG抗体,DNA壳聚糖微球疫苗的抗体水平明显高于DNA疫苗。结论壳聚糖微球疫苗投递系统可提高多表位DNA疫苗的免疫应答效果。 相似文献
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SBR反应器中反硝化条件下去除苯酚工艺 总被引:2,自引:0,他引:2
基于异养反硝化原理,在序批式间歇反应器(SBR)中对反硝化同时降解苯酚的菌种进行了149 d的驯化,驯化通过逐步提高进水中苯酚和NO3--N的浓度进行.驯化结束后,进水苯酚质量浓度达到360 mg/L,葡萄糖质量浓度达到100 mg/L,NO3--N质量浓度达到240 mg/L,水力停留时间6 h,苯酚和NO3--N去除率均大于98%.反应器运行结果表明:进水苯酚质量浓度低于720 mg/L时,SBR反应器运行稳定;高浓度NO2--N(>60 mg/L)可以严重影响微生物对苯酚以及NO3--N的去除能力,同时反应器中20.5%~23.5%的COD可被用于微生物的细胞合成. 相似文献
56.
丙型肝炎病毒多表位基因核酸疫苗的构建及其免疫原性 总被引:4,自引:0,他引:4
目的构建丙型肝炎病毒(HCV)多表位基因的真核表达载体pcDNA3.1(-)-CtEm,用该重组质粒免疫BALB/c小鼠,并检测其免疫原性。方法用BamHⅠ和HindⅢ双酶切含有HCVC区、E2区模拟表位和NS3~NS5区细胞表位串联基因的原核表达载体pQE30-CtEm,克隆入真核表达载体pcDNA3.1(-),脂质体瞬时转染CHO细胞,并检测其表达。以100μg重组质粒免疫BALB/c小鼠,检测其体液免疫和细胞免疫效果。结果所构建的真核表达载体pcDNA3.1(-)-CtEm在CHO细胞中能获得有效表达。该质粒免疫小鼠后可诱导高水平的抗体,特异性淋巴细胞增殖反应、IFN-γ水平及CTL活性均明显高于空载体和生理盐水对照组。结论已成功构建HCV多表位基因的真核表达载体pcDNA3.1(-)-CtEm,免疫小鼠后可诱导特异性体液免疫和细胞免疫应答。 相似文献
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Kaiguang Yang 《Desalination》2005,175(3):297-304
DNA-encapsulated polyethersulfone (PES) hollow microspheres are fabricated by means of a liquid-liquid phase separation technique; the hollow microspheres are then used to remove environmental pollutant organic compounds and heavy metal ions. The amounts of DNA encapsulated in the microspheres are dependent on the PES concentration, the DNA concentration used to prepare the particles, and the diameter of the syringe needle. The hollow microspheres can be used to remove harmful organic compounds including ethidium bromide (EB), acridine orange (AO) and endocrine disruptors. With the increase of the DNA amount encapsulated into the hollow microspheres, the removal ratios of these compounds increased. Additionally, the DNA-encapsulated PES hollow microspheres can selectively accumulate and remove heavy metal ions such as Ag+, Cu2+ and Zn2+ These results suggested that the DNA-encapsulated PES hollow microspheres have a potential to be used in environmental applications. 相似文献
60.
This paper is the first one of the two papers entitled “modeling and solving mixed-model assembly line balancing problem with setups”, which has the aim of developing the mathematical programming formulation of the problem and solving it with a hybrid meta-heuristic approach. In this current part, a mixed-integer linear mathematical programming (MILP) model for mixed-model assembly line balancing problem with setups is developed. The proposed MILP model considers some particular features of the real world problems such as parallel workstations, zoning constraints, and sequence dependent setup times between tasks, which is an actual framework in assembly line balancing problems. The main endeavor of Part-I is to formulate the sequence dependent setup times between tasks in type-I mixed-model assembly line balancing problem. The proposed model considers the setups between the tasks of the same model and the setups because of the model switches in any workstation. The capability of our MILP is tested through a set of computational experiments. Part-II tackles the problem with a multiple colony hybrid bees algorithm. A set of computational experiments is also carried out for the proposed approach in Part-II. 相似文献