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61.
A. Halmemies-Beauchet-Filleau A. Vanhatalo V. Toivonen T. Heikkilä M.R.F. Lee K.J. Shingfield 《Journal of dairy science》2014
Diets based on red clover silage (RCS) typically increase the concentration of polyunsaturated fatty acids (PUFA) in ruminant meat and milk and lower the efficiency of N utilization compared with grass silages (GS). Four multiparous Finnish Ayrshire cows (108 d postpartum) fitted with rumen cannulas were used in a 4 × 4 Latin square design with 21-d periods to evaluate the effect of incremental replacement of GS with RCS on milk production, nutrient digestion, whole-body N metabolism, and milk fatty acid composition. Treatments comprised total mixed rations offered ad libitum, containing 600 g of forage/kg of diet dry matter (DM), with RCS replacing GS in ratios of 0:100, 33:67, 67:33, and 100:0 on a DM basis. Intake of DM and milk yield tended to be higher when RCS and GS were offered as a mixture than when fed alone. Forage species had no influence on the concentration or secretion of total milk fat, whereas replacing GS with RCS tended to decrease milk protein concentration and yield. Substitution of GS with RCS decreased linearly whole-tract apparent organic matter, fiber, and N digestion. Forage species had no effect on total nonammonia N at the omasum, whereas the flow of most AA at the omasum was higher for diets based on a mixture of forages. Replacing GS with RCS progressively lowered protein degradation in the rumen, increased linearly ruminal escape of dietary protein, and decreased linearly microbial protein synthesis. Incremental inclusion of RCS in the diet tended to lower whole-body N balance, increased linearly the proportion of dietary N excreted in feces and urine, and decreased linearly the utilization of dietary N for milk protein synthesis. Furthermore, replacing GS with RCS decreased linearly milk fat 4:0 to 8:0, 14:0, and 16:0 concentrations and increased linearly 18:2n-6 and 18:3n-3 concentrations, in the absence of changes in cis-9 18:1, cis-9, trans-11 18:2, or total trans fatty acid concentration. Inclusion of RCS in the diet progressively increased the apparent transfer of 18-carbon PUFA from the diet into milk, but had no effect on the amount of 18:2n-6 or 18:3n-3 at the omasum recovered in milk. In conclusion, forage species modified ruminal N metabolism, the flow of AA at the omasum, and whole-body N partitioning. A lower efficiency of N utilization for milk protein synthesis with RCS relative to GS was associated with decreased availability of AA for absorption, with some evidence of an imbalance in the supply of AA relative to requirements. Higher enrichment of PUFA in milk for diets based on RCS was related to an increased supply for absorption, with no indication that forage species substantially altered PUFA bioavailability. 相似文献
62.
《Food Control》2014,35(2):703-706
The objective of this study was to evaluate the occurrence of aflatoxin M1 (AFM1) in raw milk samples from 18 dairy farms in the Yangtze River Delta region during four different seasons. A total of 72 tank milk samples was collected with 18 samples for each season. Milk AFM1 was detected using LC-MS/MS. The AFM1 was detected in 43 milk samples (59.7%) ranging in concentration from 10 to 420 ng/L. The concentration of AFM1 in raw milk was significantly higher during the winter (123 ng/L) than during other seasons (P < 0.05). There was no significant difference between the spring (29.1 ng/L), summer (31.9 ng/L), and autumn (31.6 ng/L) (P > 0.05) seasons. This indicates that raw milk collected during the winter is at high risk for AFM1 and that seasonal factors should be considered for the management of aflatoxins in both the feed and milk. 相似文献
63.
K. Mchiouer S. Bennani M. Meziane 《Energy Sources, Part A: Recovery, Utilization, and Environmental Effects》2016,38(24):3572-3577
In this study, a Lactobacillus strain was isolated from raw cow milk. It was tentatively identified at the species level according to its carbohydrate fermentation profile and phenotype characteristics as Lactobacillus paracasei KSNM. The susceptibility of the identified Lactobacillus to 23 antibiotics was performed using the disc diffusion testing method. Inhibition zone diameters were measured and expressed in terms of sensitive, intermediate or resistant. KSNM strain was sensitive toward; amoxicillin, ampicillin, amoxyclav (amoxicillin + clavulanic acid), tetracycline, macrolides, sulfamides/trimethoprim, and rifampicin. But, it expressed intermediate sensitivity toward; cefamandole, cefaclor, and cephalothin. However, it was resistant to penicillin G, aminoglycosides, quinolones, ciprofloxacin, lincomycin, nitroxolin, and a large number of cephalosporins, which included cefoxitin, cefotaxime, and ceftriaxone. 相似文献
64.
65.
Estimating teat canal cross-sectional area to determine the effects of teat-end and mouthpiece chamber vacuum on teat congestion 总被引:1,自引:0,他引:1
J.F. Penry J. Upton G.A. Mein M.D. Rasmussen I. Ohnstad P.D. Thompson D.J. Reinemann 《Journal of dairy science》2017,100(1):821-827
The primary objective of this experiment was to assess the effect of mouthpiece chamber vacuum on teat-end congestion. The secondary objective was to assess the interactive effects of mouthpiece chamber vacuum with teat-end vacuum and pulsation setting on teat-end congestion. The influence of system vacuum, pulsation settings, mouthpiece chamber vacuum, and teat-end vacuum on teat-end congestion were tested in a 2 × 2 factorial design. The low-risk conditions for teat-end congestion (TEL) were 40 kPa system vacuum (Vs) and 400-ms pulsation b-phase. The high-risk conditions for teat-end congestion (TEH) were 49 kPa Vs and 700-ms b-phase. The low-risk condition for teat-barrel congestion (TBL) was created by venting the liner mouthpiece chamber to atmosphere. In the high-risk condition for teat-barrel congestion (TBH) the mouthpiece chamber was connected to short milk tube vacuum. Eight cows (32 quarters) were used in the experiment conducted during 0400 h milkings. All cows received all treatments over the entire experimental period. Teatcups were removed after 150 s for all treatments to standardize the exposure period. Calculated teat canal cross-sectional area (CA) was used to assess congestion of teat tissue. The main effect of the teat-end treatment was a reduction in CA of 9.9% between TEL and TEH conditions, for both levels of teat-barrel congestion risk. The main effect of the teat-barrel treatment was remarkably similar, with a decrease of 9.7% in CA between TBL and TBH conditions for both levels of teat-end congestion risk. No interaction between treatments was detected, hence the main effects are additive. The most aggressive of the 4 treatment combinations (TEH plus TBH) had a CA estimate 20% smaller than for the most gentle treatment combination (TEL plus TBL). The conditions designed to impair circulation in the teat barrel also had a deleterious effect on circulation at the teat end. This experiment highlights the importance of elevated mouthpiece chamber vacuum on teat-end congestion and resultant decreases in CA. 相似文献
66.
将Lactobacillus delbrueckii subsp.bulgaricus ND02(LB-ND02)和Streptococcus thermophilus ND03(ST-ND03)按1∶1、1∶10、1∶100、1∶1000接种于脱脂乳中,同时接入益生菌Bifidobacterium lactis V9(B.lactis V9,接种量为2.0×107g-1),于42℃进行发酵。通过对发酵及贮藏过程中发酵乳指标的测定,评价LB-ND02和ST-ND03的接种比例对发酵乳品质的影响。结果表明,随着LB-ND02接种比例减小,凝乳时间显著延长,B.lactis V9活菌数显著提高。4℃贮藏28 d后,随LB-ND02接种比例减小,B.lactis V9存活率差异显著,后酸化也显著减弱。研究发现,LB-ND02和ST-ND03的接种比例,显著影响发酵乳的发酵时间、B.lactis V9活菌数、后酸化及黏度。 相似文献
67.
简要介绍了运城盐湖矿物黑泥的形成历史及其富含成分,重点考察了其在洁面乳化妆品中的应用。结果表明,含有2.0%~4.0%黑泥的洁面乳配方性质稳定、性能温和,其各项指标均符合国家相关标准规定。含有黑泥的洁面乳经人群试用后的综合评价结果良好,黑泥在洁面乳体系中具有显著的去除多余油脂和去角质功能。 相似文献
68.
In this work, the rheological and textural properties, water-holding capacity (WHC), syneresis, and microstructure of a novel formulation of Kefir were studied by varying the incubation time and temperature, as well as the ratio of peanut-milk and skimmed-milk. Generally, all the experimental responses resulted to be significantly affected (p < 0.05) by all dependent variables examined. Kefir WHC was not significantly influenced (p > 0.05) by the fermentation temperature. 相似文献
69.
Fatty acid profile of milk from goats fed diets with different levels of conserved and fresh forages
MANUELA RENNA PAOLO CORNALE CAROLA LUSSIANA VANDA MALFATTO ANTONIO MIMOSI LUCA M BATTAGLINI 《International Journal of Dairy Technology》2012,65(2):201-207
Aim of the study was to evaluate the effect of different proportions of hay and fresh grass in goats’ diet on milk fatty acid profile. Nine Camosciata goats were fed a fixed amount of concentrate (30% of total diet) and different percentages (40% vs 30%, 50% vs 20% and 60% vs 10%) of hay and fresh grass, respectively. Diminishing amounts of fresh grass percentages in the diet led to significant increases of lauric, myristic and palmitic acids (P ≤ 0.001) and to significant decreases of C18:1 t6‐11, rumenic and α‐linolenic acids (P ≤ 0.001) in milk, thus determining a worsening of the health value of milk fat. 相似文献
70.
Paolo Bergamo Giovanna Trinchese Chiara De Filippo Giorgio Gifuni Marcello Gaita Angelica Pignalosa Immacolata Donizzetti Rosalba Putti Rossella Di Palo Antonio Barletta Maria Pina Mollica 《Molecular nutrition & food research》2012,56(10):1596-1600
Dietary PUFA, mainly those of the n‐3 family, are known to play essential roles in the maintenance of energy balance and in the reduction of body fat deposition through the upregulation of mitochondrial uncoupling that is the main source of reactive oxygen species. We hypothesized that rat supplementation with raw donkey's milk (DM), characterized by low‐fat content and higher n3:n6 ratio, may affect energy balance, lipid metabolism, and prooxidant status as compared to animals treated with cow's milk. In the present study, the effects of drinking raw DM (for 4 weeks) on energy balance, lipid metabolism, antiinflammatory, and antioxidant/detoxifying defences was compared to that produced by rat intake of an iso‐energetic amount of raw cow's milk. The hypolipidemic effect produced by DM paralleled with the enhanced mitochondrial activity/proton leakage and with the increased activity or expression of mitochondrial markers namely, carnitine palmitoyl transferase and uncoupling protein 2. The association of decreased energy efficiency with reduced proinflammatory signs (TNF‐α and LPS levels) with the significant increase antioxidant (total thiols) and detoxifying enzyme activities (glutathione‐S‐transferase NADH quinone oxidoreductase) in DM‐treated animals, indicated that beneficial effects were attributable, at least in part, to the activation of nuclear factor 2 erythroid‐related factor 2 pathway. 相似文献