Improvement of the Functionality of Minced Mackerel by Hydrolysis and Subsequent Lactic Acid Bacterial Fermentation

ABSTRACT: To improve functionality and add nutritional value to mackerel, its muscle protein was hydrolyzed by the proteases from Aspergillus oryzae , and further fermented by lactic acid bacteria (LAB). Compared with minced mackerel, the antioxidative ability against linoleic acid (from 10% to 42%), α,α-diphenyl-β-picrylhydrazyl scavenging capability (from 38% to 85%), reducing power (from 0.58 to 0.83), Fe²⁺ chelating capability (from 42% to 72%), and trolox equivalent antioxidant capacity (from 2.5 to 3.5 m M ) of the hydrolysate significantly increased ( P < 0.05) after 1 h of hydrolysis and 12 h of LAB fermentation. Both hydrolysate and LAB fermented samples could stimulate the proliferation of both human hybridoma HB4C5 and mouse macrophage J774.1 cells. These data suggest that the enhancement of cell viability and antioxidative ability could be substantially improved after hydrolysis and 12 h of LAB fermentation.     

A thermal investigation on coals from Assam (India)

A thermal characterization of two coal samples from Ledo and Tikak collieries of Makum coalfield, Assam, India using XRD, FT-IR, and TGA was reported in this paper. The coal samples were heated for 20, 40 and 60 min in a 1000-watt heater (temperature ∼ 250 °C) in presence of air and characterized by XRD and FT-IR spectroscopy. Both the coals contain amorphous and crystalline phases. The raw coals also contain very small peaks due to quartz, calcite, gypsum, pyrite, and chlorite. The XRD patterns were found to change upon heating. In the coals heated for 20 and 40 min, it was observed that both amorphous and crystalline parts are common in them; crystalline part being the major one in the 40 min heated samples. The XRD patterns of the samples heated for 60 min indicate the presence of major quantities of α-quartz, hematite, and chlorite in them. They also show some new peaks, which are assigned to be kaolinite, illite, magnetite and very small in comparison to the amorphous portion in raw coals. α-quartz was found to be most stable crystalline phase of silica in the coals. The crystallinity % (X-ray) of the coals heat-treated for different times was determined and found to be increasing with time of heating. The FT-IR spectra of raw and heat-treated coal samples at 250 °C were also recorded and compared. The spectra were observed to be almost similar and it was observed that few functional groups disappear on heating at 250 °C. The same coal samples were also characterized by thermogravimetric analysis (TGA) and differential thermal analysis (DTA) techniques. On heat treatment in air atmosphere up to 800 °C, 20–27% weight loss occurs due to removal of various volatile materials. DTA results indicate the chemical reactivity of the coal sample initially at 80–110 °C due to loss of water, and two other major reactions at around 420 and 530 °C due to primary and secondary volatization.     

Comparison of three chosen vegetables with others from South East Asia for their lutein and zeaxanthin content

Three local leafy vegetables chekup manis (Sauropus androgynus), West Indian pea tree leaves (Sesbania grandiflora (L.) Pers.), and drumstick tree leaves (Moringa oleifera), are consumed by local South East Asian populations and are believed to have beneficial effects on improved vision and prevention of eye diseases. High performance liquid chromatography equipped with photodiode array detection was used to investigate their lutein and zeaxanthin contents, which were compared with those from other commonly found vegetables in the region. It was found that these three leafy vegetables contained significantly higher amounts of lutein namely, 19.5, 28.3, and 24.8 mg/100 g edible fresh leaves, respectively, compared to other vegetables in the region. It was also found that cooking in boiling water increase the extractable lutein content in chekup manis by almost 20%, within 4 min.     

Effect of previous gutting on rancidity development in horse mackerel (Trachurus trachurus) during frozen storage at −20 °C

Gutting was applied to fresh horse mackerel (Trachurus trachurus) to study its effect on rancidity development during a prolonged frozen storage (up to 12 months at −20°C). To do so, chemical (free fatty acids, FFA; peroxide value, PV; thiobarbituric acid index, TBA-i; fluorescence ratio, FR) and sensory (odour and taste) analyses were carried out. The results showed that the gutting of horse mackerel led to a higher degree of oxidation in the frozen product, according to the chemical (PV, TBA-i and FR) and sensory (odour and taste) analyses. However, a lower extent of lipid hydrolysis (FFA formation) was detected at the end of the storage (twelfth month) as a result of gutting. It is concluded that the gutting of a medium-fat fish species such as horse mackerel is not recommended as previous treatment to frozen storage.     

Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants

Total antioxidant capacities of 133 Indian medicinal plant species sampled from 64 families were assessed by ABTS, DPPH and FRAP assays, and their total phenolic contents measured by Folin–Ciocalteu assay. These species exhibited a broad range of antioxidant activities, varying from 0.16 to 500.70 mmol TEAC/100 g DW in the ABTS assay. The antioxidant activity values similarly varied with the DPPH and FRAP assays. Significant and positive linear correlations were found between total antioxidant capacities and phenolic contents (R = 0.89–0.97), indicating that phenolics were the dominant antioxidant constituents in the tested medicinal plants. Preliminary identification of the major phenolic compounds from 83 selected medicinal plants by reversed-phase HPLC revealed phenolic acids, tannins, flavonoids, curcuminoids, coumarins, lignans, and quinines. The fruit of Terminalia chebula, pericarp of Punica granatum and gall of Rhus succedanea showed very high levels of hydrolysable tannins, and the gum of Acacia catechu presented very high levels of catechin and epicatechin in addition to tannins. Major phenolics in many of the medicinal plants were identified for the first time (e.g., Euphorbia lathyrus, Ipomoea turpethum, and Picrorrhiza kurroa). This systematic investigation of a large number of Indian medicinal plants proved important for understanding their chemical constituents and functionality in Ayurvedic medicine, and contributes to the search for natural sources of potent antioxidants.     

Activity-guided isolation and identification of free radical-scavenging components from an aqueous extract of Coleus aromaticus

An activity-directed fractionation and purification process was used to identify the DPPH (l,l-diphenyl-2-picrylhydrazyl) free radical-scavenging components of Coleus aromaticus Benth. Fresh leaves of C. aromaticus were extracted with water and then separated into hexane, ethyl acetate, and water fractions. Among these, only the ethyl acetate phase showed strong DPPH radical-scavenging activity in vitro, when compared with water and hexane phases. The ethyl acetate fraction was then subjected to separation and purification using Sephadex LH-20 chromatography. Three compounds showing strong DPPH radical-scavenging activity were shown, by spectral methods (¹H NMR, ¹³C NMR, and MS) and by comparison with literature values, to be rosmarinic acid, chlorogenic acid and caffeic acid. In addition, HPLC identification and quantification of isolated compounds were also performed. Rosmarinic acid was found as a major component and principally responsible for the radical-scavenging activity of C. aromaticus.     

Superchilled,chilled and frozen storage of Atlantic mackerel (Scomber scombrus) fillets – changes in texture,drip loss,protein solubility and oxidation

Changes in quality characteristics in relation to protease activity and protein oxidation in chilled, superchilled and frozen mackerel fillets during storage were studied. The solubility of sarcoplasmic proteins was quite stable in mackerel samples for all storage experiments, whereas the solubility of myofibrillar proteins decreased in both superchilled and frozen samples. A significant correlation (r = 0.983, P < 0.05) between the increased activity of cathepsin B+L in chilled fillets and softening of the fish flesh during storage was revealed. Contrary with chilled samples, the texture of superchilled mackerel fillets became tougher along the storage period, which can be explained by a higher rate of myofibrillar oxidation (r = 0.940, P < 0.05). The hardness and drip loss decreased slightly at the end of frozen storage. Superchilling preserved the quality of mackerel fillets with the least side effects in relation to protein solubility, drip loss and softening of the fish tissue as compared to chilled and frozen storage.     

Indian thermal power plant challenges and remedies via application of modified data envelopment analysis

This paper evaluates the performance of coal‐fired thermal power plants in India for the year 2008–2009 using data envelopment analysis (DEA); subdividing the power plants into three categories depending on their scale—small, medium, and large. The classical DEA model is analyzed to identify the efficient ones from the whole gamut of plants run by various organizations of the central government, state government, and private sector. Slack analysis is carried out to explore the specific areas that need to be focused on, in quantitative terms, for the overall efficiency improvement. Further efficiency evaluation is extended from a single criterion‐based conventional approach to a multiple criteria oriented approach, and the resulting DEA models are more efficient and flexible in many aspects, particularly in discriminant and weight analysis. Results of multicriteria DEA (MCDEA) are substantiated with cross‐efficiency analysis by deploying the weights obtained by the MCDEA in the cross‐efficiency analysis. On the basis of the insights provided by the outcome of the analysis, both qualitative and quantitative measures are proposed for improvement of the plant performances. The result of this analysis may assist the management of the power plants to introspect and review their systems and processes for optimal use of resources. The methodology adopted in the present work can also be employed for deeper understanding of power plants in other parts of India as well as in other countries.