Inhibition of lipase from rice (Oryza sativa) by diethyl-p-nitrophenyl phosphate

The inhibition of rice bran lipase (RBL) by diethyl-p-nitrophenyl phosphate was studied with reference to kinetics, nature of inhibition and also elucidate the effect of the inhibitor on the structure—function of the enzyme. Enzyme activity measurements shows that the inhibitor is more effective at 0.050 mM concentration of diethyl-p-nitrophenyl phosphate and the activity is 50% at this level of inhibitor concentration. The affinity of substrate for the enzyme was observed by the increase in the velocity of the reaction with increase in the substrate concentrations and double reciprocal plot indicates that the inhibition followed a competitive in nature and inhibition constant K _i is found to be 0.016 mM at pH 7.0. The decrease in apparent thermal denaturation temperature to 4 °C compared to control indicates the destabilization of enzyme in the presence of inhibitor. Fluorescence spectral measurements suggests that pronounced quenching of fluorescence intensity of RBL occurs at higher concentrations of diethyl-p-nitrophenyl phosphate and ‘K _a’ value was found to be 2.4 × 10⁴ M⁻¹ with free energy change ΔG^o—26 kJ/mol at 30 °C suggesting strong binding between the enzyme and the inhibitor with microenvironmental changes occur at the active site or in the neighbourhood of active site. The far UV-CD data suggest that there is no significant changes in the conformation of the enzyme as a result of binding of diethyl-p-nitrophenyl phosphate. These results indicate that diethyl-p-nitrophenyl phosphate is a inhibitor of RBL and binds to the enzyme in brining about inhibition without any structural alterations.     

德国市场广场的古与今

本文以德国市场广场的历史演变为研究对象,通过对比历史中和今日生活中的市场广场号观,提出德国市场广场是城市中“一体化”．的公共空间,公共性与人的活动是贯穿其发展历程的主要脉络;正是人性化的公共空间管理及其对公共活动的高度容纳才创造了今日德国市场广场多样化的空间与景观。     

Thermodynamic modeling of gas solubility in aqueous sodium chloride solution

An electrolyte Equation of State is presented by combining the Cubic Plus Association Equation of State,Mean Spherical Approximation and the Born equation.This new model uses experimental relative static permittivity,intend to predict well the activity coefficients of individual ions (ACI) and liquid densities of aqueous solutions.This new model is applied to model water + NaCl binary system and water + gas +NaCl ternary systems.The cation/anion-water interaction parameters of are obtained by fitting the exper-imental data of ACI,mean ionic activity coefficients (MIAC) and liquid densities of water + NaCl binary system.The cation/anion-gas interaction parameters are obtained by fitting the experimental data of gas solubilities in aqueous NaCl solutions.The modeling results show that this new model can correlate well with the phase equilibrium and volumetric properties.Without gas,predictions for ACI,MIAC,and liquid densities present relative average deviations of 1.3％,3.6％ and 1.4％ compared to experimental ref-erence values.For most gas-containing systems,predictions for gas solubilities present relative average deviations lower than 7.0％.Further,the contributions of ACI,and salting effects of NaCl on gases are ana-lyzed and discussed.     

统一建模语言在纺织厂资产管理系统中的应用

为改善企业资产管理,将统一建模语言面向对象建模技术应用于纺织厂资产管理系统.剖析了以用例模型为驱动,分阶段实现系统的活动图、类图模型、顺序图和构件图的设计步骤和方法.指出:资产管理是纺织厂生产的重要组成部分,直接影响着企业的竞争力.采用先进的统一建模语言方法分析设计纺织厂资产管理系统,一方面有助于提高开发过程的规范性、合作性,保证软件系统的质量;另一方面可方便地为纺织厂资产管理工作提供软件支持.     

抗ICAM-1单克隆抗体的制备及其活性

目的制备具有生物活性的抗人细胞间黏附分子-1(ICAM-1)单克隆抗体。方法以人ICAM-1为抗原免疫BALB/c小鼠,采用杂交瘤技术并经HAT选择培养和克隆化,筛选出稳定分泌抗人ICAM-1 McAb的杂交瘤细胞株,并对McAb进行纯化。用ELISA间接法测定效价并鉴定其亚类,Western blot鉴定其抗原特异性,细胞黏附试验检测其中和活性。结果筛选出1株可稳定分泌抗人ICAM-1抗体的杂交瘤细胞株3F2,杂交瘤染色体众数为98~104。纯化后的单抗蛋白浓度为1.253 mg/ml,纯度达83.6%,效价可达2.56×105。其分泌的抗体亚型为IgG1,腹水效价达5.12×105,可与ICAM-1特异性结合,可抑制ICAM-1与淋巴瘤细胞间的黏附,具有明显的中和ICAM-1的活性。结论已成功制备出抗ICAM-1的McAb,为其进一步的研究和应用奠定了基础。     

铂类抗癌配合物构效关系的理论探讨

基于铂抗癌配合物在体内的历程和化学反应探讨了它们的结构和活性之间的关系，获得决定抗癌活性大小的主要因素，包括配合物分子的脂溶性和空间体积、构型、离去基团的反应性以及载体基团的特性。提出了铂配合物具有较高抗癌活性的结构要求①非离子型②小的空间体积③顺式构型④含脂溶性的氨（胺）载体和以Ｃｌ￣（－）或二元羧酸作离去基团。     

A New Formulation Based on Ozonated Sunflower Seed Oil: In Vitro Antibacterial and Safety Evaluation

The objective of this study was to investigate the potential in vitro biological properties of Oz.Or.Oil 30, a new formulation composed of 30% ozonated sunflower seed oil, which is believed to keep skin smooth and moisturized, supporting repair processes, tissue regeneration and re-epithelialization of wounds. The antibacterial activity, the qualitative and quantitative evaluation of the cytotoxic effect of the formulation on cultures of Vero cells and 3T3 fibroblasts showed that Oz.Or.Oil 30 merits further in vivo study using clinical-laboratory correlations, because it could be suggested as an alternative therapy against bacterial and fungal diseases.     

微粒EMD电化学活性及应用

本文从等酸点pH值、pH—MnO_2电极电位,干电池放电容量等对微粒EMD同其他MnO_2进行比较,肯定了微粒EMD的电化学活性及应用价值。     

重组人肝细胞生长因子α在大肠杆菌中的表达及活性检测

目的　建立人肝细胞生长因子α链 (rhHGFα)高效原核表达体系。方法　以pRC/CMV hHGF为模板 ,扩增hHGFαcDNA ,继以XhoI酶切为 386和 95 4bp 2个片段 ,亚克隆入pBSKS,DNA测序后 ,将上述两个片段与pBV2 2 0连接 ,构建重组质粒。转化E .coliJM10 9、DH5α和BL2 1(DE3) ,筛选高效表达菌株。分离包涵体 ,8mol/L尿素溶解 ,梯度复性后利用反相和凝胶过滤层析纯化重组蛋白 ,经MTT法检测活性。结果　所克隆的hHGFα基因序列正确 ,筛选出高效表达菌株BY ,经SDS-PAGE显示在相对分子质量 5 2 0 0 0处出现特异的目的蛋白表达带 ,表达量约占全菌蛋白的 2 5 %。表达产物以不溶性的包涵体 (IBs)形式存在 ,复性后具有刺激原代大鼠肝细胞生长的作用。结论　已成功表达了rhHGFα蛋白 ,为研究rhHGFα结构与功能及中试生产奠定了基础。     

Frequency of the sit to stand task: An observational study of free-living adults

The sit to stand movement is a key determinant of functional independence. Knowledge of the frequency with which the sit to stand movement is performed throughout the day could inform workplace ergonomics, but has rarely been examined.Healthy adults (n = 140) were recruited from the general population. Free-living activity for each participant was reported using an activity monitor. On average, participants performed 60 (±22) sit to stand movements each day. Participants in indoor sedentary occupations performed significantly more sit to stand movements per day than participants in outdoor active occupations (66 vs. 54; n = 102; p = 0.003). Participants (n = 33) performed significantly more sit to stand movements on working days than on non-working days (65 vs. 55; p = 0.018).This analysis provides contemporary data for sit to stand frequency in a predominantly working population, and demonstrates that work and employment have a significant effect on that frequency.