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21.
Leaf area index (LAI) is a key variable for the understanding of several eco-physiological processes within a vegetation canopy. The LAI could thus provide vital information for the management of the environment and agricultural practices when estimated continuously over time and space thanks to remote sensing sensors.This study proposed a method to estimate LAI spatial and temporal variation based on multi-temporal remote sensing observations processed using a simple semi-mechanistic canopy structure dynamic model (CSDM) coupled with a radiative transfer model (RTM). The CSDM described the temporal evolution of the LAI as function of the accumulated daily air temperature as measured from classical ground meteorological stations.The retrieval performances were evaluated for two different data sets: first, a data set simulated by the RTM but taking into account realistic measurement conditions and uncertainties resulting from different error sources; second, an experimental data set acquired over maize crops the Blue Earth City area (USA) in 1998. Results showed that the proposed approach improved significantly the retrieval performances for LAI mainly by smoothing the residual errors associated to each individual observation. In addition it provides a way to describe in a continuous manner the LAI time course from a limited number of observations during the growth cycle.  相似文献   
22.
Tortillas were prepared using commercial hydrocolloids, stored for 7 and 14 days and their available, resistant and retrograded resistant starches, were evaluated alongside their in vitro starch digestibility. Available starch (AS) decreased with storage time and tortillas with hydrocolloids had lower values than the control sample. Tortillas elaborated with TC-20 gum did not present substantial differences in AS. Control tortilla had resistant starch (RS) content that increased with storage time but, in general, tortillas with hydrocolloids did not show any change in RS values with storage time, except tortillas with TC-1 gum that presented a slight increase after 7 storage days. Approximately 50% of RS is due to the retrogradation phenomenon as it was shown by the amount of retrograded resistant starch (RRS). Tortillas with added hydrocolloids had lower hydrolysis percentage and the hydrolysis was slower than in the control. In general, tortillas prepared with hydrocolloids had a lower tendency for retrogradation than control tortillas; it is important to consider this to obtain tortillas with better texture and lower RS content.  相似文献   
23.
Whole maize plants at the milk and dough maturation stages were ensiled in August and October 1984, for periods of 45 and 40 days, respectively. Thirteen net bags containing herbage samples were buried at different locations within the silo to determine changes and losses during the conservation period. Thermocouple wires and small-diameter plastic pipes were connected to every bag for temperature and gas measurements. Air penetration in the upper layer was much faster than in the inner parts. Losses in dry matter of 3.9-7.4% were found in well sealed sites in the bunkers, and of up to 36% at locations where air penetrated. The rate of air penetration into the silage and temperature at various sites in the bunker were recorded. Correlation between CO2, and N2, contents in the silage was very high (r2 = 0.995) whereas oxygen levels were close to zero.  相似文献   
24.
Forty-four pre- and post-nature drying maize kernels were collected from North China Plain and assessed for fungi infection and mycotoxins contamination. The percentage of fungi infection was significantly higher in pre-nature drying samples than post-nature drying samples except for Fusariuim graminearum, which increases from 6.06% to 24.09%. Fusarium, Aspergillus, Alternaria and Trichoderma were main genera. Fusarium verticillioides (24.77%) and F. graminearum (15.08%) were predominant species, followed by Aspergillus niger (7.51%) and Aspergillus flavus (4.93%). FB1 and DON were the major mycotoxins presented in the samples, followed by ZEN. All samples showed FB1 ranging from 16.5 to 315.9 μg/kg. All post-nature drying maize kernels showed DON ranging from 5.8 to 9843.3 μg/kg, while 7 of 22 pre-nature drying samples contaminated with DON ranging from 50.7 to 776.6 μg/kg. The samples contaminated with ZEN in pre- and post-nature drying maize were 3, with the content ranging from 60.5 to 147.6 μg/kg and from 40.7 to 1056.8 μg/kg, respectively. Only 1 sample contaminated with AFB1 of 148.4 μg/kg. The occurrence of mycotoxins is highly in accordance with the incidence of the corresponding mycotoxin-producing fungi. This is the first comprehensive comparison of fungi infection and mycotoxins contamination between pre- and post-nature drying maize kernels.  相似文献   
25.
玉米须单体成分纯化及类别鉴定   总被引:1,自引:0,他引:1  
以玉米须浸膏为原料,采用萃取和液相制备色谱相结合的办法分离得到高纯度玉米须黄酮单体,并优化出纯化工艺。  相似文献   
26.
    
In this study, the co-occurrence of multiple mycotoxins in maize kernels collected from 300 households' stores in three agro-ecological zones in Tanzania was evaluated by using ultra high performance liquid chromatography/time-of-flight mass spectrometry (TOFMS) with a QuEChERS-based procedure as sample treatment. This method was validated for the analysis of the main eleven mycotoxins of health concern that can occur in maize: aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), ochratoxin A (OTA), deoxynivalenol (DON), fumonisin B1 (FB1), fumonisin B2 (FB2), HT-2 toxin, T-2 toxin and zearalenone (ZEN). From each zone one major maize producing district for home consumption was chosen and 20 villages for each district were randomly selected for sampling. All mycotoxins of health concern, except for T-2 toxin, were detected in the maize samples. Particularly high levels of AFB1 (50%; 3–1,081 μg kg−1), FB1 (73%; 16–18,184 μg kg−1), FB2 (48%; 178–38,217 μg kg−1) and DON (63%; 68–2,196 μg kg−1) were observed. Some samples exceeded the maximum limits set in Tanzania for aflatoxins or in European regulations for other mycotoxins in unprocessed maize. Eighty seven percent of samples were contaminated with more than one mycotoxin, with 45% of samples co-contaminated by carcinogenic mycotoxins, aflatoxins and fumonisins. Significant differences in contamination pattern were observed among the three agro-ecological zones. The high incidence and at high levels (for some) of these mycotoxins in maize may have serious implications on the health of the consumers since maize constitute the staple food of most Tanzanian population. Effective strategies targeting more than one mycotoxin are encouraged to reduce contamination of maize with mycotoxins.  相似文献   
27.
    
Detailed investigation on the effect of gamma (γ) irradiation on germination, sporulation, and growth of aflatoxigenic moulds (Aspergillus parasiticus 2999, Aspergillus flavus 305, and Aspergillus niger 388), as well as on the reduction of aflatoxin B1 (AFB1) level in artificially and naturally contaminated maize/feed samples was performed. The results of in vitro and in situ experiments with aflatoxigenic moulds demonstrated that 5 kGy-γ irradiation manages to prevent sporulation, germination and growth of the tested moulds both when in form of a pure and when in form of a mixed culture. In the feed samples artificially contaminated with AFB1 (50 μg kg−1) 5 kGy-γ irradiation reduced AFB1 level by around 60%, while 10 kGy-dose reduce it for around 85%. Similarly, in feed samples spiked with AFB1 in the concentrations of 100 μg kg−1 5 kGy-dose reduced the AFB1 level by approximately 70%, while the dose of 10 kGy reduced it by approximately 90%. The experiments on naturally contaminated maize samples (n = 30) confirmed these observations; following a 5 kGy-irradiation, the overall mean AFB1 reduction equalled to 69.8%, while the irradiation with a 10 kGy-dose achieved the overall mean toxin reduction of 94.5%. The obtained results indicate that γ irradiation can be used to prevent the growth of aflatoxigenic moulds and to reduce the AFB1 levels in various goods intended for animal and human consumption, thus minimizing the animal and human exposure to this carcinogenic mycotoxin.  相似文献   
28.
    
Crop-specific GMO matrices of 199 genetically modified (GM) events, comprising 143 GM maize events with 75 genetic elements and 56 cotton events with 45 genetic elements, were developed to screen globally approved GM maize and cotton events. As per the compiled information in the matrix, frequently present genetic elements were identified using GMOSeek algorithm: eight genetic elements ([P-35S] [r-act] [T-35S] [T-nos] [pinII] [pat] [aad-1] [cp4 epsps]) in maize and four ([P-35S] [T-nos] [pat] [nptII]) in cotton. Based on the cost-efficiency, feasibility of plexing and coverage of GM events, maize-specific tetraplex PCR, targeting Cauliflower Mosaic Virus 35S promoter (P-35S), Agrobacterium tumefaciens nos terminator (T-nos), Cauliflower Mosaic Virus 35S terminator (T-35S) and endogenous alcohol dehydrogenase (Adh1) gene, with limit of detection (LOD) up to 0.5% was developed. For screening of GM cotton events, pentaplex PCR, targeting P-35S, T-nos, neomycin phosphotransferase II (nptII), phosphinothricin acetyltransferase (pat) and endogenous stearoyl-ACP desaturase (Sad1) gene, with LOD up to 0.25% was developed. Practical applicability of multiplex PCR assays was confirmed with six maize samples of proficiency testing and eight spiked cotton samples. The reported tetraplex and pentaplex PCR assays could efficiently screen 94% of maize and 93% of cotton events approved globally. The developed GMO matrices in combination with multiplex PCR could facilitate checking the GM status of seed samples or food and feed products, and monitoring for presence of authorized GMOs in food and supply chain. This approach can be easily employed by low resource GM testing laboratories in the developing countries, as the multiplex PCR assays are easy to operate with less time and cost inputs. The GMO matrices being presented herein are based on the current information of approved GM events of maize and cotton, which can be further upgraded by including new approved GM events. As most of the newly approved GM events are stacked versions of already commercialized GM events, the developed multiplex PCR assays could also be employed to screen for their presence.  相似文献   
29.
    
In this study, chlorpyrifos residue levels in field crops of rice, maize and soybean were investigated according to the “Guideline on Pesticide Residue Trials” of China. On the basis of the residual results, human dietary risks were further evaluated. Chlorpyrifos residues of harvest grains were firstly prepared by QuEChERS method and analyzed using Gas Chromatography Tandem Mass Spectrometry (GC–MS/MS). Dietary risks were assessed by a deterministic approach. The median residues in field trials of rice, maize and soybean were 0.617, 0.0227 and 0.0136 mg kg−1, respectively. The highest residues in field trials of rice, maize and soybean were 3.23, 0.114 and 0.102 mg kg−1, respectively. Chronic intake assessment indicated that only 39.0% of acceptable daily intake (ADI, 0–0.01 mg kg bw−1 day−1) was consumed through rice, maize and soybean. The acute hazard indexes (aHI) of adults was 26.1% of acute reference dose (ARfD, 0–0.1 mg kg bw−1) and aHI of children was 63.5% of ARfD in dietary exposure assessment through rice, maize and soybean consumption. Single pathway risk assessment indicated that chlorpyrifos application on field crops in manner of the good agricultural practices didn't pose public health risks.  相似文献   
30.
    
A simple and rapid magnetic solid-phase extraction (M-SPE) procedure using multi-walled carbon nanotube-magnetic nanoparticles (MWCNT-MNPs) as sorbents was established for purification of zearalenone (ZEA), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalanone (ZAN), α-zearalanol (α-ZAL) and β-zearalanol (β-ZAL) in maize. The main parameters affecting the clean-up efficiency were thoroughly investigated, and high purification efficiencies for all analytes were obtained. The resulting MWCNT-MNP-ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was validated for maize samples. The matrix effects were greatly minimized using the M-SPE approach, with signal suppression/enhancement values decreased from 69.9–127.6% to 92.1–103.8%. Consequently, complex matrix-matched calibration curves were not necessary and the calibrations constructed in acetonitrile could be applied for accurate quantification of the targeted mycotoxins in real samples. The average recoveries ranged from 75.8 to 104.1% and the inter- and intra-day precision values expressed as RSDs, were all lower than 14%. Limits of detection and quantification were in the range of 0.03–0.04 and 0.07–0.10 μg/kg, respectively. The analytical performance of the developed method was also successfully evaluated with maize samples, and this method was proved to be a powerful tool for monitoring ZEA and its derivatives in maize.  相似文献   
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