N-乙酰基-S-烯丙基-L-半胱氨酸的合成及其抗辐射活性

以L-半胱氨酸为原料,经取代和酰化两步反应合成目标产物N-乙酰基-S-烯丙基-L-半胱氨酸(N-acetyl-S-allyl-L-cysteine,NAc-SAC)。以~(137)Csγ射线照射至不同剂量的ICR小鼠作为研究对象,通过30 d存活率实验、血相和免疫系统实验、各项脏器指数以及外周血淋巴细胞彗星实验,研究目标化合物分别在200、400、600 mg/kg给药剂量下的抗辐射活性。ICR小鼠经伽马射线照射至致死剂量7.5 Gy后,其存活率由单纯照射对照组的41.6%,分别提高到NAc-SAC给药组的58.3%、50.0%和66.7%,平均存活天数明显延长。~(137)Csγ射线照射至亚致死剂量6.0 Gy后,NAc-SAC在不同程度上增加了受照小鼠的脾指数、脾结节数(CFU-S)、骨髓DNA含量和白细胞数。彗星实验中7.0 Gy伽马射线照射联合NAc-SAC给药组小鼠外周血淋巴细胞的尾长、尾矩、Olive尾矩和尾部DNA百分含量明显低于7.0 Gy伽马射线单纯照射组(p0.01),提示NAc-SAC可明显降低辐射导致的DNA损伤。本研究表明,目标化合物NAc-SAC具有一定的抗辐射损伤作用。     

褪黑激素的动物睡眠试验

为研究褪黑激素对动物睡眠的作用，分别用翻正反射和σ／δ两种指标，观察了褪黑激素对小鼠和家兔的睡眠效应。结果表明，在实验剂量下褪黑激素对小鼠几乎无睡眠诱导作用，但对家兔有一定促眠效应；对戊巴比妥诱导小鼠睡眠时间有延长作用（ＥＤ５０分别为雄性７．０３ｍｇ／ｋｇＢＷ。雌性１９．０５ｍｇ／ｋｇＢＷ）；对巴比妥诱导小鼠入睡时间有缩短作用。此外，还对实验结果、方法等诸因素进行了讨论，并提出了有关动物睡眠试验的建议     

游泳时间、采血时间对小鼠血乳酸浓度的影响

为研究运动时间、采血时间对动物血乳酸浓度的影响，通过对动物的基础性研究，测得小鼠在不同游泳时间、不同运动强度下血乳酸的变化及不同取血时间对血乳酸测定值的影响。研究结果表明，小鼠在运动时随游泳时间延长及运动强度增大，血乳酸浓度逐渐升高，而后缓慢下降，随运动强度继续增大，血乳酸浓度继续升高，长时间运动后即刻取血，测得的血乳酸浓度显著高于运动后１５ｍｉｎ取血（Ｐ＜０．００１）。     

富硒胶囊对小鼠的免疫促进作用及辐射保护作用

为研究富硒胶囊对小鼠免疫能力和辐射损伤的影响,选择了检测免疫调节和抗辐射损伤的８项指标,对富硒胶囊的功效作用进行了测定。实验结果表明,２５０和７５０ｍｇ／ｋｇ剂量的富硒胶囊可提高小鼠淋巴细胞增殖能力,单核巨噬细胞吞噬能力,脾脏抗体形成细胞数和血清中抗绵羊红细胞抗体效价。另外,富硒胶囊可延长受７．５Ｇｙ＾６０Ｃｏ－γ照射小鼠平均存活时间,提高３０ｄ存活率和白细胞数。揭示富硒胶囊对小鼠具有免疫促进作用     

STIMULATING EFFECT OF LOW DOSE ~(147)Pm ON DNA REPAIR IN SPERMIOGENIC STAGES

ＳＴＩＭＵＬＡＴＩＮＧＥＦＦＥＣＴＯＦＬＯＷＤＯＳＥ~（１４７）ＰｍＯＮＤＮＡＲＥＰＡＩＲＩＮＳＰＥＲＭＩＯＧＥＮＩＣＳＴＡＧＥＳＳＴＩＭＵＬＡＴＩＮＧＥＦＦＥＣＴＯＦＬＯＷＤＯＳＥ~（１４７）ＰｍＯＮＤＮＡＲＥＰＡＩＲＩＮＳＰＥＲＭＩＯＧＥＮＩＣＳＴ...     

Adaptation Effects In The 70Th Generation Of Mice From Radionuclide-Polluted Territories In The South Urals,RUSSIA

Male Microtus arvalis mice from radionuclide-polluted territories in the South Urals ( 90 Sr activity of ground - 0.2 Ci/km 2 , 2 Ci/km 2 , 500 Ci/km 2 , 800 Ci/km 2 ) were investigated. The 90 Sr content in a mouse skeleton was determined. On intensification of protein and carbohydrate enzymes, an increase of total antioxidant activity and a reduction of lipid peroxidation processes were detected. These are the adaptation effects in the liver of Microtus arvalis mice, which are the 70th generation of animals exposed to long-term irradiation.     

Effect of Dietary Protein Level and Origin on the Redox Status in the Digestive Tract of Mice

The present study was undertaken to evaluate the effects of high protein (soybean protein or casein) on the balance between production of free radicals and antioxidant level in digestive organs of mice. For this purpose, male (C57BL/6J) mice were adapted to experimental diets containing soybean protein or casein with 20% (normal protein diets, NPDs) or 60% (high protein diets, HPDs), and HPDs supplemented with 0.06g/kg cysteamine. After two weeks of feeding, oxidative and antioxidative parameters in duodenum, liver and pancreas were measured. The results show that ingestion of high protein markedly increased contents of superoxide anion and malondialdehyde (MDA), decreased activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and Na⁺ K⁺-ATPase, and content of reduced glutathione (GSH) in digestive organs of mice (P<0.05). Levels of oxidative parameters were lower and antioxidant capacity of both enzyme and non-enzyme was higher in mice fed with soybean protein than those fed with casein. In groups fed HPDs supplemented with cysteamine, oxidative stress was mitigated. However, oxidative parameter levels were still higher than those of NPD-fed groups. The present study indicates that ingestion of high protein diets could result in an imbalance between oxidant and antioxidant, and thus induce oxidative stress in digestive organs of mice. The oxidative damage was smaller in mice fed with high level of soy protein in comparison with casein.     

芦笋抑制乳腺癌小鼠肿瘤生长的实验研究

目的：观察芦笋对小鼠原发性乳腺癌模型肿瘤的抑制作用。方法：采用BALB/c小鼠原发性乳腺癌移植到小鼠乳垫上建立小鼠乳腺癌原位移植模型,用鲜芦笋混悬液灌胃0.4ml/只,每日1次,连续20天。每周称小鼠的体重,量肿瘤大小两次;死亡小鼠当天称体重,解剖称肿瘤重量,测量肿瘤大小;第28天称体重后,处死荷瘤鼠剥离瘤块称重量,测量肿瘤大小,观察芦笋混悬液对荷瘤小鼠肿瘤的抑制作用。结果：芦笋对小鼠原发性乳腺癌有明显的抑制作用。结论：芦笋有抑制小鼠原发性乳腺癌肿瘤生长的功效。     

Caged mice mating behavior detection in surveillance videos

The purpose of this study is to develop a computer vision-based method to automatically detect the mating behavior of caged mice in surveillance videos. Previously we took advantage of our developed algorithm and analyzed the objects of mating mice in the consecutive frames, we unprecedentedly showed that, to the best of our knowledge, the mice mating behavior can be automatically detected based on video processing (Lo et al., 2009 [13]). In this paper, we proposed an improved method which monitors the distance between two mating objects and more effectively detects the mating behavior. In addition, a more detailed portrayal of the mating behavior can be further elaborated as a function of the distance patterns in the tails of two caged mice. Experimental results show that the current system can effectively detect the mice mating behavior with the highest precision rate of 96.1%, far better than that of our previously proposed method.