One-Component Artificial Gustatory System Based on Hydrogen-Bond Organic Framework for Discrimination of Versatile Analytes

Hydrogen-bond organic frameworks (HOFs) with excellent structural and luminescent properties have emerged as a promising material for the construction of fluorescence sensors. However, designing a facile, universal and high throughput sensor with multiplex detection capacity still remains challenging. Herein, a one-component sensor array is constructed that mimics natural gustatory system for accurate and high-throughput discrimination and identification of versatile analytes. HOF as a single sensing element greatly simplifies the probe preparation in sensor array and detection procedure. Metal ions, proteins and bacteria as the model targets are rapid and accurately discriminated, presenting the universality of the system. Particularly, the system is successfully used for the classification of antibiotic mechanisms. The study expands the application scope of HOFs and provides a facile and universal system for sensing applications.     

Solving cell formation and task scheduling in cellular manufacturing system by discrete bacteria foraging algorithm

We consider a joint decision model of cell formation and task scheduling in cellular manufacturing system under dual-resource constrained (DRC) setting. On one hand, machines and workers are multi-functional and/or multi-skilled, and they are grouped into workstations and cells. On the other hand, there is a processing sequence among operations of the parts which needs to be dispatched to the desirable workstations for processing. Inter-cell movements of parts can reduce the processing times and the makespan but will increase the inter-cell material handling costs. The objective of the problem is to minimise the material handling costs as well as the fixed and operating costs of machines and workers. Due to the NP-hardness of the problem, we propose an efficient discrete bacteria foraging algorithm (DBFA) with elaborately designed solution representation and bacteria evolution operators to solve the proposed problem. We tested our algorithm using randomly generated instances with different sizes and settings by comparing with the original bacteria foraging algorithm and a genetic algorithm. Our results show that the proposed DBFA has better performance than the two compared algorithms with the same running time.     

X80管线钢在含硫酸盐还原菌的土壤环境中的应力腐蚀开裂行为研究进展

X80管线钢因具有高强度、高韧性、抗脆断等性能,已成为现代油气运输中应用最为广泛的钢材之一。X80管线钢在埋地土壤环境中不可避免地受到应力和SRB(Sulfate-reducing bacteria)的共同作用,近年来有关X80管线钢在含SRB的土壤环境中的应力腐蚀开裂已成为一个研究重点。综述了应力腐蚀开裂和SRB腐蚀的影响因素,总结了关于应力和SRB协同作用对X80管线钢腐蚀行为影响的研究现状,分析了现行研究的缺陷和不足,并针对这些问题对今后的研究进行了展望。     

石油降解菌的固定化材料研究进展

随着微生物固定化技术在石油污染治理领域内发挥的作用越来越大,关于固定化微生物技术的研究越来越受到关注,而载体材料在微生物固定化技术中发挥着关键的作用。本文阐述了对固定化石油降解菌载体材料性能的要求,对无机物、天然有机物、合成高分子、复合材料等不同类型载体在石油降解菌固定化中的研究与应用以及对各类材料固定化石油降解菌的降解效果进行了综述。对比概述了各种不同载体材料对应的固定化方法,指出了各载体材料、相应固定方法的优劣势及适用场景,描述了石油降解菌固定化技术及相关载体材料今后的发展趋势。     

基于氧电极的乙醇微生物传感器的在线检测系统

乙醇氧化菌消耗利用乙醇,并产生溶解氧的特性,结合 Clark 氧电极技术设计一种新的乙醇微生物传感器检测方法.该微生物为嗜有机甲基杆菌,是以乙醇作为唯一的碳源生长的菌株.采用利于微生物细胞生长的 PVA-海藻酸盐溶胶-凝胶包埋法,对筛选、分离得到的乙醇氧化细菌固定处理,通过 Clark 氧电极对催化乙醇产生的溶解氧进行...     

A/O系统中活性污泥组成细菌的分离与鉴定

通过菌胶团破碎、混合细菌分离纯化、单一菌种生理生化特性研究等工作，从厌氧／好氧(Anoxic／Oxic，简称A／O)工艺活性污泥系统中分离出8种细菌并分别鉴定出其属别，其中普通细菌3种、亚硝化细菌4种和硝化细菌1种。在A／O工艺进水水质出现波动和需要调节出水质量的情况下，参照各种细菌的固有特性，调节和控制生产工艺参数，可迅速而有效地保证和提高A／O工艺处理化工生产废水的效果。     

生物氧化法处理含油脂废水

该文介绍油脂分解菌装置处理含油脂废水的分解原理及实例。     

Isolation of bacterial metabolites as natural inducers for larval settlement in the marine polychaete Hydroides elegans (Haswell)

The bacterial component of marine biofilms plays an important role in the induction of larval settlement in the polychaete Hydroides elegans. In this study, we provide experimental evidence that bacterial metabolites comprise the chemical signal for larval settlement. Bacteria were isolated from biofilms, purified and cultured according to standard procedures. Bacterial metabolites were isolated from spent culture broth by chloroform extraction as well as by closed-loop stripping and adsorption of volatile components on surface-modified silica gel. A pronounced biological activity was exclusively observed when concentrated metabolites were adsorbed on activated charcoal. Larvae did not respond to waterborne metabolites when prevented from contacting the bacterial film surface. These results indicate that an association of the chemical signal with a sorbent-like substratum may be an essential cofactor for the expression of biological activity. The functional role of bacterial exopolymers as an adsorptive matrix for larval settlement signals is discussed.     

重组质粒pMG36e-nisI-gfp在乳酸菌中的应用

本研究将带有nisI和咖（绿色荧光蛋白）基因的乳酸菌表达载体pMG36e-nisI-gfp转化至乳酸菌中,观察其在宿主内的稳定性．对重组菌进行了菌体形态、传代培养、质粒验证等研究,考察了该质粒的稳定性．结果显示：重组菌在不含抗生素的培养基中连续传代20代后,质粒丢失率低;菌体大小和形态基本不变;质粒经HindⅢ酶切后大小不变;GFP在第0、5、10、15和20代宿主菌中都可以表达,表达量没有明显差别,在SDS-PAGE中的带型一致;初步的动物实验验证了该质粒作为遗传标记的应用效果．说明该质粒在宿主菌中具有良好的稳定性．