厂站电能量采集终端测试系统的研制

近几年来,全国各省正在大力推进电能量计量遥测系统的建设,其中通信规约调试和终端功能测试是系统建设中一项非常重要而又费时费力的工作。针对电能计量遥测系统建设的需求研制了电能量采集终端测试系统,系统由测试通信通道装置和测试主站组成,不但可以作为调试通信规约的工具,也是终端产品验收的测试平台。该系统投入使用后,大大提高了测试工作效率,为广东地区电网电能计量遥测系统建设顺利推进发挥了重要的作用。     

T91与G102异种钢焊接接头运行前后硬度试验分析

对新焊的及运行了78000h的T91与G102（12Cr2MowVTiB）异种钢焊接接头进行了硬度试验分析。结果表明,T91／G102异种钢焊接接头（低匹配）运行前后硬度分布趋势基本一致;经长时运行后,T91管子母材区域显微硬度变化很小,而G102管子母材区显微硬度明显降低;长时运行后,T19侧的热影响区以及焊缝区的显微硬度均明显降低;长时运行后,G102热影响区出现了显微硬度的反常升高现象,具体原因还需要进行进一步研究分析。     

A Promiscuous Bacterial P450: The Unparalleled Diversity of BM3 in Pharmaceutical Metabolism

CYP102A1 (BM3) is a catalytically self-sufficient flavocytochrome fusion protein isolated from Bacillus megaterium, which displays similar metabolic capabilities to many drug-metabolizing human P450 isoforms. BM3′s high catalytic efficiency, ease of production and malleable active site makes the enzyme a desirable tool in the production of small molecule metabolites, especially for compounds that exhibit drug-like chemical properties. The engineering of select key residues within the BM3 active site vastly expands the catalytic repertoire, generating variants which can perform a range of modifications. This provides an attractive alternative route to the production of valuable compounds that are often laborious to synthesize via traditional organic means. Extensive studies have been conducted with the aim of engineering BM3 to expand metabolite production towards a comprehensive range of drug-like compounds, with many key examples found both in the literature and in the wider industrial bioproduction setting of desirable oxy-metabolite production by both wild-type BM3 and related variants. This review covers the past and current research on the engineering of BM3 to produce drug metabolites and highlights its crucial role in the future of biosynthetic pharmaceutical production.     

二氧化氯对鲜切莲藕多酚氧化酶的影响研究

以莲藕为试材,研究了二氧化氯(ClO2)对鲜切莲藕在低温贮藏期间多酚氧化酶(PPO)、褐变及感官品质影响的动态变化.试验结果表明,藕的褐变与PPO有密切关系,PPO是发生酶促褐变的关键酶.50mg/L、100mg/L浓度的ClO2处理鲜切藕片5min、10 min、15 min后,在贮藏期间能抑制PPO的活性,减轻褐变,保持好的感官品质.而10 mg/L浓度对PPO的活性不起抑制作用反而促进了PPO的活性.100 mg/L ClO2处理鲜切藕片10 min抑制PPO的活性效果最好,能使藕片在4℃下一周的时间保持最佳的感官品质和最轻的褐变度.而鲜切产品维持一周的货架寿命基本上可满足消费与生产的需求.     

SDG102, a H3K36-Methyltransferase-Encoding Gene,Plays Pleiotropic Roles in Growth and Development of Maize (Zea mays L.)

Although histone lysine methylation has been studied in thale cress (Arabidopsis thaliana (L.) Heynh.) and rice (Oryza sativa L.) in recent years, its function in maize (Zea mays L.) remains poorly characterized. To better understand the function of histone lysine methylation in maize, SDG102, a H3 lysine 36 (H3K36) methylase, was chosen for functional characterization using overexpressed and knockout transgenic plants. SDG102-deficiency in maize caused multiple phenotypes including yellow leaves in seedlings, late-flowering, and increased adult plant height, while the overexpression of SDG102 led to reduced adult plant height. The key flowering genes, ZCN8/ZCN7 and MADS4/MADA67, were downregulated in SDG102-deficient plants. Chromatin immunoprecipitation (ChIP) experiments showed that H3 lysine 36 trimethylation (H3K36me3) levels were reduced at these loci. Perturbation of SDG102 expression caused the misexpression of multiple genes. Interestingly, the overexpression or knockout of SDG102 also led to genome-wide decreases and increases in the H3K36me3 levels, respectively. Together, our results suggest that SDG102 is a methyltransferase that catalyzes the trimethylation of H3K36 of many genes across the maize genome, which are involved in multiple biological processes including those controlling flowering time.     

基于专用加密芯片的单片机软件加密系统设计

针对单片机程序代码保密性较差的缺陷,介绍了一种利用专用加密芯片实现单片机软件加密的方案。系统以MSP430F5438单片机为平台,采用Atmel公司的专业安全加密芯片AT88SA102S,将单片机中的程序代码与特定的加密芯片绑定,从而实现了单片机代码的不可复制性。最后介绍了基于HEX文件读写的加密芯片密钥管理系统软件设计。     

基于有源RFID的极低功耗温湿度传感标签的设计

提出了可以显著降低功耗的有源RFID标签工作流程,设计出一种极低功耗的温湿度传感标签。该标签采用微控制器PIC24F16KA102为核心,以温湿度传感器SHT21S和射频收发芯片nRF24L01为外围器件,完成温湿度及电池电量信息的检测和发送。该有源标签在开阔场地的有效阅读距离可以达到80m左右,标签连续工作时的电池寿命为9年以上,具有体积小、寿命长等优点,可广泛应用于生产车间的温湿度监测、冷链物流、医疗系统、仓储物资管理等方面。     

屏式过热器爆管原因分析

通过采用化学成分分析、宏观检验和显微组织分析等方法对石家庄热电有限公司屏式过热器爆管试样进行了分析,并通过氧化皮厚度估算了其当量温度,计算出屏式过热器剩余寿命。分析认为屏式过热器爆管为长时间超温运行,造成材料组织老化及腐蚀引起,建议运行中加强高温金属部件入厂检验、在线监测及寿命评估等,以确保机组安全运行。     

102规约向IEC61850规约转换网关的研究

随着数字化变电站的发展,如何实现采用IEC61850协议的新系统和基于原有协议IEC60870-5-102规约的现役系统的兼容成为现实问题。由面向点信息的传统规约向面向对象技术的IEC61850规约转换也是智能电网发展的必然趋势。通过比较分析IEC61870-5-102规范和IEC61850标准的差异,提出了一种在当前系统与新系统之间过渡的通信网关模型,以保证变电站内非IEC61850装置与IEC61850新系统的兼容。     

硼在12Cr2MoWVTiB钢高压锅炉管热制管及服役过程中的变迁

用α－粒子径迹显微照相技术，并行电子能量损失谱（ＰＥＥＬＳ）分析电镜技术和金相试验方法，研究了１２Ｃｒ２ＭｏＷＶＴｉＢ（１０２）钢铸态、开坯态（锻造、初轧后）、轧管态（穿孔、连轧、张减后）、热处理态（正火、回火后）及服役态（蠕变、持久试验后）硼元素的存在形式和分布规律。研究表明，铸态（本样为例）硼元素随机分布：晶内固溶，轻度晶界富集。经锻造或初轧开坯后，硼的分布渐趋均匀。穿孔、张减等重复热加工过程使硼出现晶界的偏聚迹象：正火使晶界硼元素明显偏聚，同时伴随Ｃｒ的富集。正火加回火使硼大量向晶界迁移并形成富Ｃｒ的Ｍ_（２３）（Ｂ，Ｃ）_６，其后的服役状态（蠕变或持久试验），含硼的Ｍ_（２３）（ＢＣ）_６碳化物重新溶解而使硼回到晶粒内部并逐步均匀化。研究指出，硼的晶界析出与富Ｃｒ的Ｍ_（２３）（Ｂ，Ｃ）_６相有密切关系，即当（Ｆｅ，Ｃｒ）_（２３）Ｃ_６存在于晶界时，易形成（Ｆｅ，Ｃｒ）_（２３）（Ｃ，Ｂ）_６型碳化物析出（硼相）。１０２钢中硼含量和铬含量及热处理工艺是影响硼元素迁移行为的两大主导因素。