微波提取黄芩甙的协同效应研究

以水为介质,进行了微波协同提取黄芩甙的研究,找到了微波协同提取黄芩甙的较佳工艺条件:m(水)∶m(黄芩)=15∶1,时间6min,微波功率680W提取2次,过滤,滤液用质量分数为15%的盐酸调pH=1～2,析出沉淀,过滤,滤饼用水洗和乙醇洗,干燥即得黄芩甙粗产品,收率约6 6%,黄芩甙质量分数约为86 3%。与传统水提法比较,具有提取时间短,收率高等优点。     

Application of genetic algorithm to self-organizing fuzzy controller in fed-batch culture ofScutellaria baicalensis G. plant cell

A self-organizing fuzzy controller is constructed for control of substrate concentration in fed-batch operation of a cell culture process. A genetic algorithm is used to generate fuzzy rules of the self-organizing fuzzy controller and to modify the universe of discourse automatically. The fuzzy controller is designed for the application ofScutellaria baicalensis G. plant cell culture process as a model system. A substrate feeding strategy of a two stage culture method to maximize flavone glycoside production in fed-batch culture ofScutellaria baicalensis G. plant cell is proposed based on structured model of growth and product synthesis. As a two stage culture, the feeding strategy consists of the first period at 22 g/L of glucose concentration to promote cell growth and the second period at 10 g/L of glucose concentration to promote flavone glycoside synthesis. The designed self-organizing fuzzy controller is applied to regulate the glucose concentration at a given set-point to increase flavone glycoside synthesis. The simulation results show that the proposed feeding strategy in a fed-batch culture enhances flavone glycoside production and the self-organizing fuzzy logic controller generated by genetic algorithm improves controller performance.     

响应面法优化半支莲黄酮提取工艺

应用响应面法优化半支莲黄酮提取条件,并考察其清除自由基能力。结果表明：最佳提取工艺参数为提取时间62min、液料比32:1(mL/g)、提取温度69℃,此条件下半支莲黄酮提取率为0.442%,其对DPPH自由基清除率达到95.4%。     

黄芩茎叶中总黄酮提取工艺优化

以黄芩茎叶为原料,采用醇提法提取黄芩茎叶总黄酮。在单因素试验的基础上,以提取温度、提取时间、乙醇体积分数和料液比为考察因素,采用L9(34)正交试验探讨醇提法提取黄芩茎叶总黄酮工艺。最佳工艺条件：以70%乙醇为溶剂、提取温度70℃、料液比1:18(g/mL)、提取3次、每次2.0h,此时黄芩茎叶提取物中总黄酮含量为21.02%,总黄酮得率1.39%。     

滇黄芩总黄酮酶解超声提取工艺及抗氧化活性研究

该文研究了滇黄芩总黄酮酶解超声提取的工艺条件及总黄酮的抗氧化活性。结果表明,纤维素酶解超声工艺对滇黄芩总黄酮的提取具有协同作用,提高总黄酮提取得率。在超声功率100 W条件下,通过单因素和正交试验,得出酶解超声最佳工艺条件为提取时间4 h、乙醇体积分数55%、加酶量50 U/g（干原料）、液料比30∶1（mL∶g）、提取温度50 ℃,在此条件下,平均提取得率为24.03%。抗氧化实验研究表明,滇黄芩总黄酮对超氧阴离子和羟自由基的清除率IC50分别为0.14 mg/mL、0.20 mg/mL。     

黄芩对真丝织物染色的研究

通过正交试验对黄芩中色素的提取及其对真丝织物染色、媒染工艺进行了研究，并讨论了关键影响因素，同时对染色试样进行了牢度测试。     

黄芩提取工艺的优化研究

采用正交实验法研究中草药黄芩有效成分提取的最佳条件。结果表明,当乙醇体积含量为40％,热回流时间1．0h,提取剂用量为黄苓用量的10倍时,提取效果最佳。同时考察了热回流次数对黄芩苷收率的影响,得出最佳热回流次数为3次。