川东北沙溪庙组天然气地球化学特征及地质意义 ——以五宝场地区为例

川东北地区侏罗系沙溪庙组浅层致密气具有投资成本低、见效快、分布广、资源量大、埋深浅等诸多优势,是一个高效的勘探开发对象.通过对川东北五宝场地区侏罗系沙溪庙组天然气与下伏烃源岩实地采集,进行了相关的天然气组分、碳同位素、镜质体反射率、显微组分以及总有机碳测定等地球化学实验,根据天然气和烃源岩样品分析,对川东北侏罗系沙溪庙...     

四川盆西平原区裸土扬尘中Pb、Cd化学形态的生物有效性

为探究四川盆西平原区裸土扬尘中Pb、Cd总质量分数与其生物有效性的规律,在构建的"阳离子型污染物土壤表层运移行为研究系统"内进行模拟起尘试验,对土壤样品进行Pb、Cd模拟污染试验,结果表明:试验条件下,四川盆西平原区裸土扬尘颗粒物中Pb、Cd的总质量分数随着粒径的减小而增大,其中Pb的化学形态以碳酸盐结合态为主(44....     

川南城镇密集区协调发展的规划对策研究

川南城镇密集区一体化快速、协调发展的规划对策为:促进区域产业协调发展、协同发展旅游业、合理引导城镇群空间发展格局、加强区域空间管制、促进区域基础设施的整体协调建设、合作共建区域环境安全体系.     

《城市规划管理技术规定》的编制与实施

《城市规划管理技术规定》应由政府组织编制,在广泛征求公众的意见后依法报批;应坚持公平原则,不断补充完善和更新其内容,不可随意规定用地兼容性.《城市规划管理技术规定》的内容应以"强制性"指标为主,可适当放宽对"非强制性"指标的规定,范围应覆盖整个城市规划区.     

低损害钻井液体系的性能及在川西低渗气藏的应用

针对川西低渗气藏储层特性及损害机理,在重质碳酸钙类刚性颗粒表面接枝部分交联的聚合物制得储层保护剂SMRP-1,将其与井壁修补强化剂等复配制得低损害钻井液.室内评价了SMRP-1的阻渗性、酸溶性、与常见水基钻井液体系的配伍性,考察了低损害钻井液的各项性能,并在川西陆相低渗气藏储层江沙209HF井进行了现场应用.结果表明,...     

Identification of Novel Pepper Genes Involved in Bax- or INF1-Mediated Cell Death Responses by High-Throughput Virus-Induced Gene Silencing

Hot pepper is one of the economically important crops in Asia. A large number of gene sequences, including expressed sequence tag (EST) and genomic sequences are publicly available. However, it is still a daunting task to determine gene function due to difficulties in genetic modification of a pepper plants. Here, we show the application of the virus-induced gene silencing (VIGS) repression for the study of 459 pepper ESTs selected as non-host pathogen-induced cell death responsive genes from pepper microarray experiments in Nicotiana benthamiana. Developmental abnormalities in N. benthamiana plants are observed in the 32 (7%) pepper ESTs-silenced plants. Aberrant morphological phenotypes largely comprised of three groups: stunted, abnormal leaf, and dead. In addition, by employing the combination of VIGS and Agrobacterium-mediated transient assays, we identified novel pepper ESTs that involved in Bax or INF1-mediated cell death responses. Silencing of seven pepper ESTs homologs suppressed Bax or INF1-induced cell death, five of which suppressed both cell death responses in N. benthamiana. The genes represented by these five ESTs encode putative proteins with functions in endoplasmic reticulum (ER) stress and lipid signaling. The genes represented by the other two pepper ESTs showing only Bax-mediated cell death inhibition encode a CCCH-type zinc finger protein containing an ankyrin-repeat domain and a probable calcium-binding protein, CML30-like. Taken together, we effectively isolated novel pepper clones that are involved in hypersensitive response (HR)-like cell death using VIGS, and identified silenced clones that have different responses to Bax and INF1 exposure, indicating separate signaling pathways for Bax- and INF1-mediated cell death.