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Thermal Inactivation Kinetics of Peroxidase and Lipoxygenase from Broccoli, Green Asparagus and Carrots 总被引:4,自引:0,他引:4
ABSTRACT: Thewermal inactivation curves for peroxidase (POD) and lipoxygenase (LOX) in broccoli (florets), green asparagus (tip and stem), and carrots (cortex and core) extracts were determined in the range of 70 to 95 °C for 0 to 600 s. The capillary tube method was used to obtain quasi-isothermal conditions. The kinetics of both enzymes showed a biphasic first-order model, while at 70 °C, LOX in asparagus showed a monophasic first-order behavior. LOX activity was not detected for carrots. Kinetic parameters, k and Ea , were determined for heat-labile and heatresistant isoenzyme fractions. Additionally, initial and residual activities for both enzymes within tissue sections showed a different distribution and heat stability. 相似文献
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An-An Zhang Bu-Er Ha Chang Chen Ming-Qiang Xu Qing-Hui Wang Long Xie Zhi-An Zheng Jing-Shou Zhang Wei-Qiao Lv Hong-Wei Xiao 《Journal of food science》2024,89(1):202-216
Vacuum steam pulsed blanching (VSPB) was employed as a novel blanching technology on Cornus officinalis to soften the tissue for subsequent coring and dehydration. The current work aims to explore its effect on mass transfer behavior, PPO inactivation, drying characteristics, physicochemical properties, antioxidant capacity, and microstructure of C. officinalis. Results showed that VSPB increased water loss, decreased solid gain, and increased weight reduction with increased blanching cycles. Besides, VSPB significantly changed physical properties and extensively reduced drying time which was attributed to the cell wall components dissolving and cell turgor pressure decreasing, also verified by observing microstructure alteration. PPO was completely denatured after blanching in 6 cycles, but phenolic compounds were still diffused or degraded. Notably, the content of flavonoids and antioxidant capacity significantly increased compared to fresh samples probably due to increased extractability caused by the disrupting cell structure. Besides, the carotenoids and ascorbic acid could be well preserved. 相似文献
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Polyphenol oxidase inactivation and vitamin C degradation kinetics of Fuji apple quarters by high humidity air impingement blanching 总被引:1,自引:0,他引:1
Jun‐Wen Bai Zhen‐Jiang Gao Hong‐Wei Xiao Xiao‐Tuo Wang Qian Zhang 《International Journal of Food Science & Technology》2013,48(6):1135-1141
In this study, polyphenol oxidase (PPO) and vitamin C were used as the indicators of enzymes and nutrients to evaluate the apple quality during high humidity air impingement blanching (HHAIB) process. The PPO can be completely inactivated within 7 min at 90–120 °C and can retain relatively more vitamin C in the case of PPO fully inactivation. PPO inactivation followed zero‐order kinetics model at 90 and 100 °C, and followed first‐order fraction model at 110 and 120 °C. Activation energy (Ea) of PPO inactivation was between 11.61 and 13.66 kJ mol?1 by Arrhenius equation. Vitamin C degradation under all processing temperatures was well described by first‐order model and its Ea value was 26.69 kJ mol?1. Therefore, the HHAIB process was proved to be an effective pretreatment for Fuji apple quarters to inactivate PPO fast and meanwhile to maintain produce quality. 相似文献
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研究了真空油炸紫薯片的预处理工艺.应用单因素试验及正交试验设计方法,对切片厚度、漂烫条件、冷冻时间等真空油炸紫薯片质量的预处理因素进行了系统研究,确定了真空油炸紫薯片的最佳预处理工艺:切片厚度3.0 mm;漂烫温度95℃,漂烫时间3.5 min;在-20℃条件下,冷冻18 h;在-0.094~-0.098 MPa真空度条件下,90℃真空油炸15 min.真空油炸紫薯片最大程度地保存了紫薯的营养价值,花青素含量为1.54 mg/g,脂肪质量分数为17.40%,破碎力为468.9 N.产品带有清晰的纹理,口感酥脆,无油腻感. 相似文献
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试验研究了双孢白蘑菇干燥前微波与高温瞬时蒸汽对多酚氧化酶(PPO)活性以及Vc的影响。通过微波强度(w/g)、微波处理时间(t1)、蒸汽强度(Pa/g)、蒸汽处理时间(t2)的单因素试验,设计正交试验,对PPO、Vc进行综合评价分析,得出最佳的试验条件:微波强度为5.656w/g、微波处理时间(t1)为120s、蒸汽强度为2.69Pa/g、蒸汽处理时间(t2)为80s,综合评分值最高。并对微波与高温蒸汽联合漂烫最优结果与热水漂烫处理、纯微波、纯高温蒸汽漂烫进行了比较,体现了微波与高温蒸汽联合漂烫的优势。 相似文献