Quantitative detection method for Roundup Ready soybean in food using duplex real‐time PCR MGB chemistry

BACKGROUND: Methodologies that enable the detection of genetically modified organisms (GMOs) (authorized and non‐authorized) in food and feed strongly influence the potential for adequate updating and implementation of legislation together with labeling requirements. Quantitative polymerase chain reaction (qPCR) systems were designed to boost the sensitivity and specificity on the identification of GMOs in highly degraded DNA samples; however, such testing will become economically difficult to cope with due to increasing numbers of approved genetically modified (GM) lines. Multiplexing approaches are therefore in development to provide cost‐efficient solution. RESULTS: Construct‐specific primers and probe were developed for quantitative analysis of Roundup Ready ^® soybean (RRS) event glyphosate‐tolerant soybean (GTS) 40‐3‐2. The lectin gene (Le1) was used as a reference gene, and its specificity was verified. RRS‐ and Le1‐specific quantitative real‐time PCR (qRTPCR) were optimized in a duplex platform that has been validated with respect to limit of detection (LOD) and limit of quantification (LOQ), as well as accuracy. The analysis of model processed food samples showed that the degradation of DNA has no adverse or little effects on the performance of quantification assay. CONCLUSION: In this study, a duplex qRTPCR using TaqMan minor groove binder‐non‐fluorescent quencher (MGB‐NFQ) chemistry was developed for specific detection and quantification of RRS event GTS 40‐3‐2 that can be used for practical monitoring in processed food products. Copyright © 2010 Society of Chemical Industry     

对羟基苯甲酸丁酯的安全性及其应用瓶颈

通过实验数据的对比讨论了对羟基苯甲酸丁酯用作食品防腐剂时的安全性,主张采用相对性毒理指标评价食品防腐剂的安全性,根据尼泊金丁酯在抗菌性,安全性、广谱性三方面的综合优势,建议尽快将尼泊金丁酯及其钠盐纳入GB2760范围.     

快速检测技术在食品监测中的研究进展

快速检测技术与传统检测方法相比,具有检测时效快、检测方法简单等优点,其在保障食品安全中起到非常重要的作用。文章重点叙述了食品中化学有害物质、微生物、农药残留、重金属离子的快速检测技术的研究进展。     

细胞毒理学在食品领域中的应用

细胞毒理学是研究外源性物质对生命细胞损伤作用规律及其机制的一门毒理学分支学科,它可以用于食品安全毒理学的评价、食品中有害物质的毒作用机理研究、食品加工生产中有毒有害物质的检测、功能性食品中功效成分的活性研究等。细胞毒理学的研究方法有多种,用于探讨外源物对细胞的不同作用,包括对细胞的活性影响和遗传学影响、造成细胞形态学变化以及细胞凋亡检测。目前在食品领域较广泛应用的细胞毒理学方法有MTT比色实验、细胞DNA合成测定法、彗星电泳法、Western blot法、流式细胞术测定法、NRU染色法、免疫细胞化学染色技术等。     

用油脚脂肪酸制取粘胶短纤油剂

介绍了以油脚脂肪酸为主要原料制取粘胶短纤油剂的制备原理，实验方法和生产应用。     

食品接触用多层复合包装中迁移物质的非靶向筛查和风险评价

目的 以食品接触用纸/铝/塑复合包装为研究对象,结合应用非靶向高通量筛查技术和安全风险评价方法,全面研究这类多层复合包装在接触食品过程中的迁移风险。方法 采用顶空气相色谱-串联质谱、气相色谱-四极杆飞行时间质谱和液相色谱-四级杆飞行时间质谱全面筛查食品接触用纸/铝/塑复合包装在不同迁移条件下的挥发性、半挥发性和非挥发性迁移物质,基于商业数据库、实验室自建库及行业信息解析物质化学结构及可能来源,利用内标半定量迁移水平,结合国内外食品接触材料法规和安全风险评估方法进行符合性评价及风险评价。结果 20批纸/铝/塑复合食品包装在体积分数为4%的乙酸模拟物中未发现迁移风险,在体积分数为95%的乙醇模拟物中共计检出35种物质,可能源于溶剂、抗氧剂、爽滑剂、低聚物、抗氧剂氧化/降解产物等物质。其中,33种物质经法规评估及毒理学评估,显示风险较低;其余2类聚烯烃低聚物因结构难以确定、缺乏毒理学研究建议持续关注毒理学研究进展及国内外法律法规动态。结论 通过结合应用非靶向高通量筛查技术和安全风险评估方法研究表明,在通常的使用条件下,纸/铝/塑多层复合包装用于盛装果汁等水性食品时不存在安全风险,但接触高乙醇含量或油脂类食品时建议关注聚烯烃类低聚物的迁移安全性。     

利用真空衰减法测试预包装食品的密封性

目的 预包装食品的密封性关系到食品质量与安全。本文针对预包装食品目前普遍采用水检气泡法时灵敏度不足的问题,采用真空衰减法检测食品包装的密封性。方法 分别利用2种不同检测技术对市面上55款预包装食品样品进行密封性检测,并根据包装类型、封合方式、包装规格等不同分类方式将测试数据进行综合比较分析。结果 水检气泡法测试成本低,可明显地指示出泄漏位置,但方法灵敏度较低,且无法准确定量;真空衰减法可在不破坏原有包装的情况下,大幅度提高检测灵敏度,并得到定量数据,但无法准确指示泄漏点位。结论 真空衰减法可以作为水检气泡法的有效补充方法在实际工作中使用。     

用户需求驱动下的国潮点心食物设计研究

目的 了解用户需求,有助于精准开发出符合用户需求的食物,对国潮点心的食物设计进行思考,提出基于Kano-AHP-AD模型的国潮点心食物设计研究方法。方法 运用Kano模型对国潮点心的20项需求指标进行统计和分析,确定国潮点心的需求属性划分;引入AHP法进行权重求解,明确国潮点心食物设计不同属性相对重要的用户需求;为了匹配生产中的设计参数,基于AD理论的独立性公理原则完成国潮点心功能域与设计域之间的映射,通过矩阵逐步判断设计合理性和最优解。结果 通过权重求解确定七项国潮点心重要用户需求项,得出满足用户需求的国潮点心应具备的设计需求参数,提出国潮点心的食物设计创新路径。结论 该设计方法通过定性和定量的结合,将主观的概念量化为具体数值,为国潮点心的食物设计提供思路。结论具有直观准确与科学合理的优势,可为后续国潮点心开发提供可靠的参考和科学的理论支持。     

Exploitation of the Antibacterial Properties of Photoactivated Curcumin as ‘Green’ Tool for Food Preservation

In the search for non-chemical and green methods to counteract the bacterial contamination of foods, the use of natural substances with antimicrobial properties and light irradiation at proper light waves has been extensively investigated. In particular, the combination of both techniques, called photodynamic inactivation (PDI), is based on the fact that some natural substances act as photosensitizers, i.e., produce bioactive effects under irradiation. Notably, curcumin is a potent natural antibacterial and effective photosensitizer that is able to induce photodynamic activation in the visible light range (specifically for blue light). Some practical applications have been investigated with particular reference to food preservation from bacterial contaminants.     

When organic products are tasty: Taste inferences from an Organic = Healthy Association

Previous research has consistently demonstrated that organic food is typically seen as healthier. The aim of the present study is to investigate how these health inferences influence taste perceptions of organic food. In Study 1, we show that a neutral food product with an organic label is perceived as more healthy than the same product without such a label. This higher level of perceived healthiness is paired with an improved perceived taste. In Study 2 and 3, we obtain evidence in Dutch and US samples that an organic label increases perceived taste and attractiveness of healthy (but not unhealthy) food. Whereas previous studies have shown general health halo effects of organic labels, this perspective cannot explain the specific pattern of our results, which speaks towards an ‘organic = healthy = tasty’ intuition.