一种新型三维敏感效应电容式SO2气体传感器设计

传统气体传感器只能以简单一维扩散方式对气体响应,扩散机理是建立在表面效应基础上的。提出了一种新型电容式SO2气体传感器。该传感器敏感结构由离散的微型圆柱体单元三维式扩散模型组成,模型上表面和侧面同时接触被测气体,扩大了与被测气体的接触面积。根据气敏薄膜的扩散响应理论,对传感器的响应时间作出分析,并与梳状电容式气体传感器特性相比较,说明新型传感器具有响应时间更短的优点。     

基于击键特征的手机用户身份认证系统

针对目前手机的高普及、高智能和安全性差的问题,统计并研究了不同手机用户使用手机的击键特征。依据数理统计知识发现其符合正态分布,进而设计和实现了基于击键特征的手机用户身份认证系统。经测试表明,该系统能显著提高手机安全性。     

利用地统计分析及GIS技术实现流域面雨量预报

根据地统计学及地理信息系统(GIS)的空间建模原理,采用工业标准的、开放的、统一的ArcObjects嵌入式组件开发方法,利用多模数值预报,研制并构建精细化的流域面雨量集合预报模型及雨涝区划单元定量降水应用组件,将其动态、无缝地嵌入到GIS应用系统中,自动生成子流域及雨涝区划面雨量预报指导产品.     

浅析X荧光成份分析仪在水泥生产过程中的应用

阐述了X荧光成份分析仪在水泥生产过程运行的现状及其测量原理,结合现代新型干法大规模生产线对产品质量的控制要求,对X荧光成份分析仪在水泥生产过程应用的必要性进行了说明。     

Construction of human monoclonal single-chain Fv antibodies against small-cell lung cancer by phage display libraries derived from cell-immunized SCID mice engrafted with human peripheral blood lymphocytes

In this study, we describe a phage display strategy to obtain human monoclonal single-chain Fv (scFv) antibodies binding target cancer cell surface proteins. By developing a cancer cell immunization protocol for SCID mice engrafted with human peripheral blood lymphocytes in combination with an antibody phage display method, we have isolated phage antibodies binding small-cell lung cancer cell line H889 by subtractive selection. One of the isolated scFv antibodies, 12EAb, recognized the E2 component of pyruvate dehydrogenase complex (PDC-E2) by immunoprecipitation according to MALDI-TOF MS analysis. Furthermore, we have confirmed the plasma membrane localization of PDC-E2 in small-cell lung cancer cells by immunocytochemistry and cell surface protein biotinylation, although PDC-E2 is usually located in the mitochondrial matrix. These results, including unique localization of identified antigens, were obtained by proteomic approaches. The present methods can be applied to generate human monoclonal scFv antibodies against tumor cells and to identify new molecular targets for immunotherapy and markers for diagnosis.     

Isoform-specific expression and characterization of 14-3-3 proteins in human glioma tissues discovered by stable isotope labeling with amino acids in cell culture-based proteomic analysis

Human 14-3-3 proteins have isoform-specific expression and functions in different kinds of normal or tumor cells and tissues. However, the expression profiling of 14-3-3 proteins and isoform-specific biological functions are unclear in human glioma so far. In our study, the expression levels and characterization of 14-3-3 isoforms in human glioma tissues were investigated by a sensitive, accurate stable isotope labeling with amino acids in cell culture-based quantitative proteomic strategy. As a result, except unexpressed 14-3-3σ, the other six isoforms, with different expression levels, were existed in glioma tissues and para-cancerous brain tissues (PBTs). 14-3-3β and η were upregulated, whereas 14-3-3ζ was downregulated in glioma tissues compared with that in PBTs. And the other three isoforms 14-3-3ε, θ, and γ had similar expression levels in human glioma tissues and PBTs. Western blot and immunohistochemistry analysis were both consistent with the quantitative proteomic data. The loss of expression of 14-3-3σ was further discovered due to DNA high methylation in its coding region in glioma by methylation-specific PCR analysis. These results indicated that the four isoforms, including 14-3-3β, η, ζ, and σ, may play important roles in tumorigenesis of human glioma, which is probably used as potential biomarkers for diagnosis and targets for treatment of human gliomas in future.     

白光LEDs用(Ba/Sr/Ca)2.9Ce(PO4)3:0.1Eu2+的发光特性

采用高温固相法制备了(Ba/Sr/Ca)_2.9Ce(PO₄)₃:0.1Eu²⁺系列荧光粉,研究了材料的发光特性。在400 nm光激发下,增大x值,Ba_2.9-xSr_xCe(PO₄)₃:0.1Eu²⁺的主发射峰由515 nm红移至540 nm,色坐标从(0.312,0.607)变到(0.376,0.580);随着y值的增大,Ba_2.9-yCa_yCe(PO₄)₃:0.1Eu²⁺的主发射峰由515 nm红移到553 nm,色坐标从(0.312,0.607)变到(0.427,0.535),随z值的增大,Sr_2.9-zCa_zCe(PO₄)₃:0.1Eu²⁺的主发射峰值由540 nm红移...     

Promote Intratumoral Drug Release and Penetration to Counteract Docetaxel-Induced Metastasis by Photosensitizer-Modified Red Blood Cell Membrane-Coated Nanoparticle

The red blood cell membrane (RBCm) provides tight protection, lowers the immunogenicity, and prolongs the circulation time of drugs in vivo when acting as the coating of drug delivery systems. However, the cellular uptake and release of drugs are hindered by RBCm. Docetaxel (DTX) is the first-line medicine for treating triple-negative breast cancer (TNBC), but it induces tumor metastasis. To solve these dilemmas, in this study, the photosensitizer 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR)-modified RBCm (DM) is prepared, which is coated onto a hybrid micelle consisting of the prodrugs of DTX and the anti-metastasis agent calcitriol (CTL), obtaining a nanoparticle, named HDC-DM. In a 4T1 tumor-bearing mouse model, after injecting HDC-DM, the intratumoral DTX and CTL concentrations are increased by 1.7 and 2.5 times compared with the free drug groups. After irradiating tumors with near-infrared laser, DiR elicits the photothermal effect, triggering the rupture of RBCm and drug release, promoting drug penetration in tumors, and inducing immunogenic cell death. The tumor growth inhibition rate is 77%, and the formation of lung metastases is reduced by 82%, with good biocompatibility. It is suggested that the combination of phototherapy, chemotherapy, and anti-metastatic therapy using HDC-DM is expected to be a powerful strategy for treating TNBC.     

Cell Development Enhanced Bionic Silk Hydrogel on Remodeling Immune Pathogenesis of Spinal Cord Injury via M2 Polarization of Microglial

Due to the complex spatial-temporal pathophysiology of spinal cord injury (SCI), effective modulation of SCI-specific inflammatory pathogenesis to achieve desirable therapeutic effects on functional recovery still remains challenging. Herein, cell-enhanced photocrosslinked silk fibroin hydrogels with extracellular matrix-mimicking cues of mechanical properties and RGD (Arg-Gly-Asp) signals are gelled in situ to fill the lesion site to modulate injury-induced neuroinflammation and promote neurite regrowth after SCI. The bionic hydrogel system provides biomimetic mechanical cues to promote neuronal differentiation of neural stem/progenitor cells (NPCs) and neurite growth by activating YAP nuclear expression. Importantly, favored by the strong capacity of silk fibroin hydrogels on macrophage/microglia recruitment, NPCs encapsulated hydrogel (NPCs@SFRGD0.1) effectively promotes recruited macrophages/microglia to M2 polarization in the lesion site by releasing S100A4 and thereby remodels the inflammatory microenvironment after SCI. Moreover, NPCs@SFRGD0.1 successfully reduces glial scar formation and accelerates corticospinal tract axon regrowth to improve locomotor recovery. Overall, this work contributes to illustrating the therapeutic mechanism of NPCs development based biomaterial therapies on modulating inflammatory microenvironment and this NPCs enhanced silk fibroin hydrogel provides a promising therapeutic strategy for SCI.     

Hierarchical and Hybrid Cell Load Balancing in 5G Heterogeneous Mobile Networks

In Fifth Generation (5G) Heterogeneous Mobile Networks (HetNets), deploying dense small cell networks makes user association more challenging. The process of collecting cell load information from the User Equipments (UEs) and broadcasting the feedback message involves significant overhead and time complexity. Moreover, the UEs may not know the optimum cell to reselect, satisfying its data rate requirements. In order to overcome these drawbacks, in this paper, we propose to design an Hierarchical and Hybrid Cell Load Balancing (HHCLB) technique using Selective Handoff. In this technique, the UEs of each cell are grouped into clusters depending on their proximity distance. Each cluster contains a cluster controller (CC) which is in charge of determining the intra-cell load and redirecting the cell-reselection request of a UE. If the data rate of any UE in a cluster becomes less than its required rate, then the cell reselection process is performed. By simulation results, it is shown that load balancing can be done proactively (implicitly) by the CCs when the load is unbalanced or can be done on demand (explicitly) when a UE send a request for cell reselection. In the case of Macro cells, HHCLB attains 71% higher throughput for low load scenario and 59% higher throughput for high load scenario. Similarly, in the case of Femto cells, HHCLB attains 19% higher throughput for low load scenario and 27% higher throughput for high load scenario.