Deconstruction of the human connexin 26 hemichannel due to an applied electric field; A molecular dynamics simulation study

Connexins are a 21-member membrane protein family constituting channels evolved in direct communication between adjacent cells by passaging cytoplasmic molecules and ions. Hexametrical assembly of connexin proteins in plasma membrane forms a wide aqueous pore known as connexin hemichannel. These hemichannels mediate cytoplasm and extracellular milieu communication both in many external tissues and in the central nervous system. In this study, a series of molecular dynamics simulations has been performed to investigate the effect of applied static and alternating electric fields on the stability and conformation of human connexin26 hemichannel. The root mean square deviations of C-alpha atoms, the dipole moment distribution, the number of inter-protein hydrogen bonds and the number of water-protein hydrogen bonds were used to assess connexin26 hemichannel stability. In the static field case, our results show that although the lowest field used in this study (0.1 V/nm) does not lead to the hemichannel deconstruction, stronger fields (>0.1 V/nm), however, disrupt the protein structure during the simulations time period. Furthermore, in the alternating case, compared to static field case, field effects on the connexin26 hemichannel conformation are reduced and consequently the protein maintains its native structure for longer times. Specifically, for the highest frequency used in this study (50 GHz), the hemichannel keeps its structure even under the effect of the strongest field (0.4 V/nm). According to our results, the protein secondary structure is preserved in the characteristic times determined for the protein deconstruction. Consequently, we suggest that the protein deconstruction is due to the tertiary and quaternary structure loss.     

论深圳大运体育中心的解构特征

通过了解解构主义建筑的背景和发展历程,展开了对解构主义建筑创作观念、创作形式的理性探讨,并以深圳大运体育中心—＂水晶石＂为代表性建筑进行拓展,力图对解构主义建筑的建筑创作手法及建筑的特征进行全面解读.     

论解构主义建筑的形式特征——以深圳市当代艺术馆与规划展览馆为例

20世纪中叶解构主义思潮出现以来,解构主义在全球范围内,对当代社会造成了广泛的冲击．解构主义建筑思潮,改变了人们对传统建筑空间、建筑表皮形式的经典认知,解构主义把所谓完整、和谐的建筑整体作解构处理,然后重新组合,创造一种感性的、破碎的空间和形态．以深圳市当代艺术馆与规划展览馆为代表性建筑,展开了对解构主义建筑创作手法及形象特征的理性探讨．     

《最蓝的眼睛》的解构主义解读

作为一种认识论和批评方法,解构主义为我们提供了一种特定的研究与解读小说文本的视角。以这一特定的视角分析当代美国黑人女作家托尼.莫里森的《最蓝的眼睛》,可以更深刻、更透彻地理解美国黑人在白人文化主导下的社会生存状态和精神困惑。     

“文革视觉风格设计”对现代平面设计的影响

运用设计的手段对文革视觉文化进行再利用和再创作而产生的视觉设计艺术.其设计手法主要是解构主义,通过解构文革视觉元素,将其从政治意识形态的语境中解脱出来,消解其中的政治意味,使之俚俗化,以后现代的面目重新出现,从而获得新的艺术生命,并赋予其新时代的复杂含义.     

A manual-based psychodynamic therapy for treatment-resistant borderline personality disorder.

The authors introduce a manual-based treatment, labeled dynamic deconstructive psychotherapy, developed for those patients with borderline personality disorder who are most difficult to engage in therapy, such as those having co-occurring substance use disorders. This treatment model is based on the hypothesis that borderline pathology and related behaviors reflect impairment in specific neurocognitive functions, including association, attribution, and alterity that form the basis for a coherent and differentiated self. Dynamic deconstructive psychotherapy aims to activate and remediate neurocognitive self-capacities by facilitating elaboration of affect-laden interpersonal experiences and integration of attributions, as well as providing novel experiences in the patient-therapist relationship that promote self-other differentiation. Treatment involves weekly individual sessions for a predetermined period of time and follows sequential stages. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Interiors of 1900. Photographs by Adolf Mas

Abstract

The markings associated with the physical wear and tear of denim garments are an integral aspect of material culture, pursued as creative concepts through deconstructed fashion approaches. For some designers a textile's heritage and the effects of time are as important as reworking silhouettes from history. While fashion is inherently forward thinking, it is the patina of age that comes from “wearing” a garment that can be integral to the designer's artistic vision. Denim garments possess unique possibilities for articulating the human form in ways that transcend the vagaries of the latest cut, through evidencing (or simulating) practices of wearing and concepts of longevity. This paper explores denim's capacity to act as a canvas for the body through analysis of a series of conceptual and commercial approaches drawn from contemporary fashion and textile designers, who have reinforced denim's origins or reinterpreted it through creative experimentation. The study is underpinned by the author's empirical research, which seeks to reveal both the recognizable and more abstract visual qualities embodied within denim as a fashion construct. Denim is evaluated as a surface for conveying complex visual signifiers relating to temporality, demonstrated through the cloth's unique facility to embody narrative and illusion.     

Sharrock and Button ... and Much Ado about Nothing

The paper discusses Sharrock's and Button's criticism of our attempt to use Habermas' communicative action theory to analyze group work platforms. We demonstrate that they misconstrue our goals of the paper, misinterpret our analysis of Habermas' action types, and misunderstand the concept of critical science. At the end we question the usefulness of these types of debates in furthering CSCW research.     

基于链路重构—解构的端到端网络链路时延推测研究简

基于网络时延推测的2个假设、时延推测模型和路径时延数据采集方法,提出了一种基于链路重构—解构的端到端网络链路时延推测方法,应用伪似然估计将原整体问题分解为若干独立子问题分别求解,利用链路重构—解构确定可求解的推测单元,控制平均采样精度和减少推测单元链路数,从而显著降低计算复杂度。通过基于模型的计算和基于NS2的仿真实验研究,验证了推测方法的准确性和有效性。