The Feasibility of Immobilization of Bioflocculant-producing Bacteria Using Mycelial Pellets as Biomass Carriers

Bioflocculant-producing bacteria Agrobacterium tumefaciens F2 and Bacillus sphaeicus F6 were immobilized onto mycelial pellets to investigate the bioflocculant-producing potential of this combined organism and the corresponding flocculating efficiency. The atomic force microscope (AFM) images of mycelial surface indicate that the mycelia pellet can immobilize bioflocculant-producing bacteria F2 and F6 as a biomass carrier. The flocculating efficiency of bioflocculant produced by this combined organism was studied under the optimum flocculating conditions obtained by Response Surface Methodology (RSM). The fermentation yield of the combined mycelial pellet is about 2.6 g/L, which is higher than that of the free bacteria (only 2.2 g/L). Flocculating efficiency of the combined mycelial pellet was comparable with that of bioflocculant generated by the free bacteria. The bioflocculant yield is enhanced and the flocculating efficiency of the co-culture is uninfluenced after immobilized with mycelial pellet as a carrier. In conclusion, the mycelial pellet is feasible as a biomass carrier for the immobilization of bioflocculant-producing bacteria.     

基于主色偏差矩阵的图像检索方法

颜色特征是图像检索领域中最为重要的视觉特征之一.对于基于颜色的图像检索系统,其性能在很大程度上取决于颜色特征的描述和匹配方法的有效性.本文使用主色偏差矩阵来描述颜色特征,并使用相关系数进行相似性度量.经实验证明,该方法对于图像的几何变换和空间变换,具有较高的检索查全率.     

基于城市污水好氧颗粒污泥脱氮除磷系统种群多样性研究

采用16S rDNA克隆文库方法对城市污水脱氮除磷好氧颗粒污泥的细菌种群进行了多样性研究.从16SrDNA克隆文库中随机挑选110个克隆子测序并进行了BLAST比对.结果表明:好氧颗粒污泥序批式反应器(GSBR)系统中细菌群落具有高度多样性,包含14个类群,优势细菌类群为Proteobacteria类群(变形菌类群),占85.18%;细菌类群优势顺序为β-Proteobacteria、α-Proteobacteria、γ-Proteobacteria、uncultured Bacteroidetes、Candidatedivision TM7、δ-proteobacterium、Firmicutes、Planctomycetacia、Actinobacteria、Sphingobacteria、Flavobacteria、Cytophagia、Uncultured bacterium和Uncultured anaerobic bacterium.初步分析了不同细菌类群在脱氮除磷系统中的作用,其中,Proteobacteria纲中部分为聚磷菌,Acidovorax sp.、Planctomycetacia、Cytophagia、Flavobacteria对氮的脱除均有一定作用.     

浮床组合生物技术对湖泊富营养化水体氮磷去除效果试验研究

试验采用浮床栽培香根草、在浮床周围悬挂人工介质、并定期向水体中投加光合细菌的组合技术,对湖泊富营养化水体进行了试验研究.试验结果表明,浮床组合生物技术对湖泊富营养化水体中的总氮、总磷具有明显的去除效果,能显著改善富营养化水体的水质;同时试验结果表明通过向水体中投放鱼类来延长生物链,更有利于提高浮床组合生物修复技术对富营养化水体的净化能力.     

基于模式聚类的二级电压控制分域方法研究

为更好解决二级电压控制分域问题,研究一种基于可视化自组织特征映射网络聚类技术的分域方法.根据自组织特征映射网络的拓扑保持、信息融合和高维数据矢量量化的特性,建立系统电压分域可视拓扑映射模型,以拓扑映射图为基础进行二级电压控制分域.在此基础上,提出以熵作为测度搜索主导节点.该分域算法融合了节点电压、相角和灵敏度等信息,利于分区间的控制解耦.该方法可离线训练,在线计算.     

微生物处理含钒废水中V5+、Cr6+的实验研究

对含钒废水处理现状进行了分析，采用复合功能菌对钒制品废水中V^5＋、Cr^6＋重金属离子进行处理。结果表明：用微生物工艺流程处理含钒废水中V^5＋、Cr^6＋重金属离子技术上可行；V^5＋、Cr^6＋去除率最高达99．9％；出水金属离子浓度达到国家排放标准。     

硅酸盐细菌在资源工程中的应用研究现状

对硅酸盐细菌的特性及在矿物加工领域中的应用现状作了较全面的综述；重点概括了硅酸盐细菌用于分解矿物、提取有价金属、矿石除杂、浮选、水处理等矿物加工方面的最新进展；同时，对硅酸盐细菌的解矿机理及在溶矿方面应用存在的问题进行了分析。     

Fecal Coliform Removal within a Marshland Upwelling System Consisting of Scatlake Soils

The marshland upwelling system (MUS) was installed on private camps in the Grand Bay National Estuarine Research Reserve, Moss Point, Mississippi. The system was evaluated for its effectiveness in removing fecal coliforms from settled, raw wastewater. A suite of studies was performed at flow rates of 1.9, 2.8, and 5.5 L/min and an injection frequency of 30 min every 3 h to investigate fecal coliform removal. An additional study was performed at a flow rate of 2.8 L/min and an injection frequency of 15 min every hour. Overall, the MUS consistently maintained fecal concentrations below effluent regulatory standards for shellfish harvesting waters (14 most probable number of colonies per 100 mL). Mean influent concentrations of 55,269±2,218,016 colony forming units (CFU)/100 mL were reduced to effluent counts of 2.7±14.07 CFU/100 mL (observed in the 1.5 m wells). Three- to four-log reductions in influent counts were observed over the initial 1.4 vector?m from the injection well. The overall removal followed a first-order decay relationship with respect to vector distance, resulting in removal rate constants ranging from 5.6 to 6.6/m and predicted surface concentrations approaching 0 CFU/100 mL. The 2.8 L/min for 30 min every 3 h treatment provided the best effluent quality.     

聚合物驱采油污水处理研究进展

含聚丙烯酰胺污水是一类比较复杂、特殊的污水,随着各个油田相继采用聚合物驱以提高原油采收率,采油污水的数量在逐年增加,其处理已成为一个亟待解决的问题。综述了采用几种方式降解水中聚丙烯酰胺的可行性研究结果,光催化氧化、硫酸盐还原菌都能使水中聚丙烯酰胺有不同程度的降解。光催化氧化考察了催化体系、光源体系等对HPAM的影响。采用1%的催化剂TiO2使用中压汞灯在紫外光的作用下,可使聚丙烯酰胺(HPAM)分解至8%左右。硫酸盐还原菌在油藏环境条件pH为7左右时,大量繁殖生长,使HPAM大幅度降解。在高温下(60～80℃),聚丙烯酰胺失稳发生降解,高温及高矿化度油藏也会导致HPAM降解。以上研究为解决聚合物采油污水的处理奠定了良好的基础。     

乳品中活性益生菌选择性检测方法的研究

研究了含活性益生菌乳品中嗜热乳链球菌、保加利亚乳杆菌、嗜酸乳杆菌、双歧杆菌和干酪乳杆菌的选择性计数方法。结果表明：ST—琼脂培养基适合嗜热乳链球菌的计数；LC—琼脂培养基适合干酪乳杆菌的计数；MRS—NNLP琼脂培养基适合双歧杆菌的计数；MRS—pH5．2琼脂培养基适合保加利亚乳杆菌的计数；MRS—柳醇琼脂或MRS—山梨醇琼脂可用于嗜酸乳杆菌和干酪乳杆菌的计数；若产品中只含有嗜酸乳杆菌和干酪乳杆菌时，可先测其总数，再用LC—琼脂培养基选择计数干酪乳杆菌数，两者之差即为嗜酸乳杆菌数。