Box-Behnken Design a Key Tool to Achieve Optimized PCL/Gelatin Electrospun Mesh

Hybrid electrospun nanofibers of polycaprolactone (PCL)/gelatin are considered as drug-delivery systems for increasing the treatment efficacy in superficial (skin) wounds. Continuous delivery of therapeutic agents, skin extracellular matrix similarity, management of wound exudate, and antimicrobial barrier effect are the major advantages of electrospun nanofibers in skin applications. Additionally, combining the favorable properties of PCL and gelatin, regarding their biocompatibility, biodegradability and mechanical performance have been revealed promising parameters to be considered for blend in hybrid structures. However, the usual optimization protocol of nanofibers’ production in electrospinning is based on the observation of one-variable-at-time being this methodology expensive and time-consuming. Therefore, in this research work, a statistical model based on four input variables namely, the flow rate, the needle-working distance, the applied voltage, and the ratio of PCL in the solution, is developed to predict the behavior of nanofibers. The performance of nanofibers is monitored by measurements of fiber's diameter, mesh's thickness, and mesh's permeability. Overall, the model showed to be statistically significant (p-value < 0.05) and an independent analysis validated the predicted response for optimal condition. Finally, a delivery study is performed to evaluate the electrospun mesh performance as a drug carrier.     

Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice

Gamma rays and electrons with kinetic energy up to 10 MeV are routinely used to sterilize biomaterials. To date, the effects of irradiation upon human acellular dermal matrices (hADMs) remain to be fully elucidated. The optimal irradiation dosage remains a critical parameter affecting the final product structure and, by extension, its therapeutic potential. ADM slides were prepared by various digestion methods. The influence of various doses of radiation sterilization using a high-energy electron beam on the structure of collagen, the formation of free radicals and immune responses to non-irradiated (native) and irradiated hADM was investigated. The study of the structure changes was carried out using the following methods: immunohistology, immunoblotting, and electron paramagnetic resonance (EPR) spectroscopy. It was shown that radiation sterilization did not change the architecture and three-dimensional structure of hADM; however, it significantly influenced the degradation of collagen fibers and induced the production of free radicals in a dose-dependent manner. More importantly, the observed effects did not disrupt the therapeutic potential of the new transplants. Therefore, radiation sterilization at a dose of 35kGy can ensure high sterility of the dressing while maintaining its therapeutic potential.     

HP300型与PYB1750型圆锥破碎机的应用

HP300型圆锥破碎机系进口设备,与国产PYB1750型圆锥破碎机比较,破碎粒度均匀,安装维修方便,备品备件少,可实现自动清腔,并且具有良好过铁保护功能;不足之处,设备一次性投资大,备件成本高。     

面齿轮精密磨削砂轮数控修整技术研究

为改善面齿轮磨削加工精度,提出了一种面齿轮磨削砂轮数控修整及误差影响分析方法。基于面齿轮传动原理,进行了面齿轮碟形砂轮磨削运动分析。依据砂轮齿廓特点,选择金刚滚轮修整,并计算金刚滚轮的运动轨迹曲线,提出了一种数控刀位点和步长的选取方式。分析了砂轮型面误差类型,给出了误差条件下的面齿轮齿面方程。借助MATLAB和CATIA软件对面齿轮齿面点进行拟合,获得了砂轮型面误差对面齿轮型面影响的变化规律。最后进行了砂轮修整和面齿轮磨削加工试验,通过对面齿轮齿面进行检测,得到面齿轮全齿面法向偏差范围-10.1um～12um。结果验证了砂轮修整方法的可行性,为面齿轮的工程化应用奠定基础。     

一种壳聚糖基敷料的制备及其性能研究

近年来,临床上由于压疮、溃足等现象而导致伤口感染病例日益增加,而目前尚未有好的解决方法或是存在对应的"金标准"产品。在选用伤口敷料上具有潜力的天然高分子材料——壳聚糖,将其与一种力学性能优良、生物相容性好的聚合物P (LLA-CL)复合,通过静电纺丝技术制备了纳米纤维膜。经过一系列表征,该纳米纤维膜被证实具有纳米级的结构、微小的空隙、良好的力学性能以及较好的生物相容性,有望运用于伤口敷料。     

CVD金刚石在齿轮磨齿机中的应用探究

介绍了CVD金刚石的发展现状及其在滚轮行业中的应用,概述了CVD金刚石的性能特点及应用领域,着重介绍了在齿轮磨齿机滚轮行业中的应用.包括CVD品级的选取,切割形状的设计,与滚轮的匹配等,为行业相关从业者提供参考.     

Development of bacterial cellulose incorporating silk sericin,polyhexamethylene biguanide,and glycerin with enhanced physical properties and antibacterial activities for wound dressing application

Bacterial cellulose (BC) has been applied for wound dressing application. In this study, physical and biological properties of the BC dressing were improved by incorporation of silk sericin (SS), polyhexamethylene biguanide (PHMB), and glycerin. The glycerin incorporation reduced dehydration rate and wound adhesion of the BC dressing in a concentration-dependent manner. PHMB, an antiseptic agent, provided antibacterial activity against Gram-positive and Gram-negative bacteria. Meanwhile, SS would enhance collagen and tissue formation in wounds. Finally, we confirmed that the BC dressing incorporating SS, PHMB, and glycerin was safe to be used as a medical material according to ISO 10993-6 standard.     

Development of silver nanoparticles‐loaded calcium alginate beads embedded in gelatin scaffolds for use as wound dressings

Silver nanoparticles (AgNPs)‐loaded calcium alginate beads embedded in gelatin scaffolds were developed to sustain and maintain the release of silver (Ag⁺) ions over an extended time period. The UV irradiation technique was used to reduce Ag⁺ ions in alginate solution to AgNPs. The average sizes of AgNPs ranged between ca 20 and ca 22 nm. The AgNPs‐loaded calcium alginate beads were prepared by electrospraying of a sodium alginate solution containing AgNPs into calcium chloride (CaCl₂) solution. The AgNPs‐loaded calcium alginate beads were then embedded into gelatin scaffolds. The release characteristics of Ag⁺ ions from both the AgNPs‐loaded calcium alginate beads and the AgNPs‐loaded calcium alginate beads embedded in gelatin scaffolds were determined in either deionized water or phosphate buffer solution at 37 °C for 7 days. Moreover, the AgNPs‐loaded calcium alginate beads embedded in gelatin scaffolds were tested for their antibacterial activity and cytotoxicity. © 2014 Society of Chemical Industry     

A Thermo‐ and Moisture‐Responsive Zwitterionic Shape Memory Polymer for Novel Self‐Healable Wound Dressing Applications

Developing wound dressings that have strong adhesion strength without causing any conglutination to the wound site is still challenging. Herein, is proposed that zwitterionic shape memory polymers can be applied as promising candidates for wound dressing. Sulfobetaine methacrylate (SBMA) is copolymerized with 2,3‐dihydroxypropyl methacrylate (DHMA) in the presence of boric acid as a cross‐linking agent. The prepared material exhibits multi‐stimuli responsive shape memory behaviors: it can rapidly return to its initial shape upon heating to 90 °C, and a gradual recovery is also observed by absorbing moisture in humid environments. The shape memory effect can be well adjusted via incorporation of sodium chloride to induce the dissociation of electrostatic interactions between PSBMA chains, leading to reduced transition temperature and faster shape recovery rate. Moreover, the dynamic nature of boron ester bonds and electrostatic interaction endows the material with effective and rapid self‐healing ability. It is also demonstrated that the deployment process of the dressing that a sample with an initially circular shape can perfectly fit and tightly bind to the wound site after moisture‐induced shape recovery. The proposed zwitterionic polymer can possibly extend the application scope of shape memory polymers and pave a new way for the design of wound dressings.