Swift and Reliable “Easy Lab” Methods for the Sensitive Molecular Detection of African Swine Fever Virus

African swine fever (ASF) is a contagious viral hemorrhagic disease of domestic pigs and wild boars. The disease is notifiable to the World Organisation for Animal Health (OIE) and is responsible for high mortality and serious economic losses. PCR and real-time PCR (qPCR) are the OIE-recommended standard methods for the direct detection of African swine fever virus (ASFV) DNA. The aim of our work was the simplification and standardization of the molecular diagnostic workflow in the lab. For validation of this “easy lab” workflow, different sample materials from animal trials were collected and analyzed (EDTA blood, serum, oral swabs, chewing ropes, and tissue samples) to identify the optimal sample material for diagnostics in live animals. Based on our data, the EDTA blood samples or bloody tissue samples represent the best specimens for ASFV detection in the early and late phases of infection. The application of prefilled ready-to-use reagents for nucleic acid extraction or the use of a Tissue Lysis Reagent (TLR) delivers simple and reliable alternatives for the release of the ASFV nucleic acids. For the qPCR detection of ASFV, different published and commercial kits were compared. Here, a lyophilized commercial kit shows the best results mainly based on the increased template input. The good results of the “easy lab” strategy could be confirmed by the ASFV detection in field samples from wild boars collected from the 2020 ASFV outbreak in Germany. Appropriate internal control systems for extraction and PCR are key features of the “easy lab” concept and reduce the risk of false-negative and false-positive results. In addition, the use of easy-to-handle machines and software reduces training efforts and the misinterpretation of results. The PCR diagnostics based on the “easy lab” strategy can realize a high sensitivity and specificity comparable to the standard PCR methods and should be especially usable for labs with limited experiences and resources.     

线型聚能切割技术爆破拆除高耸筒形钢结构物

介绍了用线型聚能切割技术定向爆破拆除易燃易爆环境下高耸筒形钢结构物的成功经验.着重介绍了切割器的选择、爆破切口形式与参数、起爆网路、安全技术措施、爆破效果等,为类似工程提供有实用价值的参考依据.     

复合土工膜在山区渠道防渗中的应用

高寒山区强透水的地基上，按常规采用粘土铺盖，浆砌石、混凝土渠道防渗质量不易保证，设计中经方案比较，选用复合土工膜防渗铺董，防渗效果显，值得推广应用。     

压缩机组气路系统工艺流程图快速生成软件的开发与应用

针对通用化工透平压缩机运行性能在线监测与评估系统的特殊需要,基于Windows系统,成功实现了对各种复杂类型压缩机工艺流程主界面动态显示的快速生成操作平台。流程图编辑工作简单、快捷;内部接口的统一,方便了对DCS数据的实时更新、分析处理与控制输出。成功为多套压缩机在线热力性能先进控制及优化分析提供了完善的图形与数据支持。     

具有良好加工性的双马来酰亚胺树脂体系的配方优化设计

为获得具有良好加工性的双马来酰亚胺树脂体系, 本文中运用二次回归正交设计方法安排实验, 建立树脂组分配比与树脂性能之间的回归方程。运用逐步搜索法求解回归方程, 实现了树脂性能的预测, 并对树脂组分配比进行优选, 从而得到了一种具有良好成型工艺性和优良的热、力学性能的双马树脂体系。      

铝质易开盖生产技术

介绍了铝质量开盖对铝合金材料性能的要求,着重分析了铝质量开盖生产过程中的技术要求,以及铆钉成形原理,刻痕的工艺的控制。指出了铝质易开盖的现状和发展趋势。     

Aerocool高透湿机织物的开发与性能分析

利用棉纤维与Aerocool纤维混纺织造机织面料。设计不同原料比例、不同组织结构等工艺参数,着重讨论了Aerocool高透湿机织物的吸水、快干、透湿性能。试验发现：Aerocool纤维含量是影响织物吸湿排汗性能的重要指标;织物内部松紧结构对织物吸湿透湿性能影响较大;不同排列比对织物吸湿性能影响不大。     

矿物解离度与工艺粒度关系和解离难易度探讨

矿物解离是选矿的必要条件之一,矿物工艺粒度、磨矿粒度以及矿物力学性质等诸多因素对矿物解离度都有影响,这些因素致使解离度和工艺粒度之间关系复杂化。本文以电炉渣黑钛石工艺粒度和解离度关系为例,研究矿物解离度与工艺粒度及矿石磨矿粒度之间的关系。并通过不同矿石破磨解离实例,探讨了矿石解离难易程度分类,用以表示矿物力学性质对解离度的影响。     

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v文章对低压易漏地层钻井液漏失机理及堵漏机理进行了探讨。对于低压易漏地层，安全钻井的关键是建造高质量的人工井壁，提高井壁的承压能力。根据地层物性规律，要求堵漏材料的尺寸范围较宽，有利于不同的颗粒尺寸的堵漏材料进行有机组合，封堵不同喉径的孔隙和各种尺寸大小的裂缝，形成稳定的人工井壁。结合现场的实际应用，提出了低压易漏地层安全钻井的技术措施。     

低生长速度下定向凝固Nd13.5Fe81.5B5永磁合金的磁取向

采用液体金属冷却(LMC)的定向凝固技术，研究了在较低晶体生长速度(12和20μm／s)条件下得到的Ndl3.5Fe81.5B5永磁合金的磁取向。分析发现，Nd2Fe14B的易磁化轴[001]仍然与柱状晶生长方向垂直，即与其晶体择尤生长方向垂直。在一定的区域内，相邻柱状晶的易磁化轴互相平行，从而组成易磁化轴平行排列的晶粒集团。当晶体生长速度从20μm／s降低到12μm／s时，晶粒集团的平均尺寸明显增大，所含晶粒数增加。