紫外线电离型SF6气体检漏仪

本论述了应用紫外线电离法进行SF6气体检测的原理,并阐述了SF6检测仪关键部件的电路结构,同时也简明地叙述了应用软件技术进行非线性处理,零点飘移及仪器标定方法。     

广西老堡铅锌矿褶皱形成机制及其控矿规律

老堡铅锌矿床主要赋存在陡山沱组白云岩或老堡组硅质岩中,顺层产出,受地层与褶皱的双重控制。矿区主干褶皱为一复式向斜,由2个次级向斜和1个次级背斜组成。从构造几何学、运动学角度,运用现代测试技术,对矿区的小构造、显微构造和组构进行构造分析后揭示,老堡褶皱构造的形成机制是以弯滑作用为主、兼具弯流作用的纵弯褶皱作用的产物。铅锌矿体主要赋存在褶皱的转折端虚脱部位及两翼滑移量较大的层间滑动破碎带中。     

折叠翼的结构非线性颤振分析

由于折叠机构间隙的存在,折叠翼的刚度特性呈非线性关系特性,工程实践中需要有效的方法,建立并求解折叠翼的非线性气动弹性方程。该文提出了弦向多个自由度具有结构非线性刚度的折叠翼颤振分析方法,并对典型折叠翼面进行了非线性颤振分析。利用动态子结构方法建立了非线性气动弹性方程,分别在频域和时域给出了颤振求解方法。对比工程软件的计算结果,验证了该方法是否满足工程计算精度的要求。通过对典型折叠翼的计算得到以下结论。在弦向多个位置具有初载间隙型非线性的典型折叠翼结构在一定速度范围内会产生稳定的极限环振荡,在一定速度范围内结构响应与初始扰动相关,后缘铰链振动相比于前缘更加剧烈,初载角较大时振荡更加剧烈。     

Oxidative Folding of Peptides with Cystine‐Knot Architectures: Kinetic Studies and Optimization of Folding Conditions

Bioactive peptides often contain several disulfide bonds that provide the main contribution to conformational rigidity and structural, thermal, or biological stability. Among them, cystine‐knot peptides—commonly named “knottins”—make up a subclass with several thousand natural members. Hence, they are considered promising frameworks for peptide‐based pharmaceuticals. Although cystine‐knot peptides are available through chemical and recombinant synthetic routes, oxidative folding to afford the bioactive isomers still remains a crucial step. We therefore investigated the oxidative folding of ten protease‐inhibiting peptides from two knottin families, as well as that of an HIV entry inhibitor and of aprotinin, under two conventional sets of folding conditions and by a newly developed procedure. Kinetic studies identified folding conditions that resulted in correctly folded miniproteins with high rates of conversion even for highly hydrophobic and aggregation‐prone peptides in concentrated solutions.     

Observing Proteins as Single Molecules Encapsulated in Surface‐Tethered Polymeric Nanocontainers

Protein unfolding inside immobilized polymerosomes : One of the most interesting properties of polymeric vesicles is their remarkable stability against extreme temperatures and osmotic stress, and their longevity even under harsh environmental conditions. We have demonstrated, in an application on protein folding, that surface‐tethered polymerosomes are suitable for performing time‐resolved single molecule studies with encapsulated proteins, as illustrated here.

     

Accepting its random coil nature allows a partial NMR assignment of the neuronal Tau protein

A combined strategy to obtain a partial NMR assignment of the neuronal Tau protein is presented. Confronted with the extreme spectral degeneracy that the spectrum of this 441 amino acid long unstructured protein presents, we have introduced a graphical procedure based on residue type-specific product planes. Combining this strategy with the search for pairwise motifs, and combining the spectra of different Tau isoforms and even of peptides derived from the native sequence, we arrive at a partial assignment that is sufficient to map the interactions of Tau with its molecular partners. The obtained assignments equally confirm the absence of regular secondary structure in the isolated protein.     

水力循环澄清池的技术改造

张掖沙湾水厂的水力循环澄清池是上世纪70年代初建造。由于池深,各部分功能不匹配,动能有效利用率低,排泥不畅,对水量、水质变化适应性差,限制了产水量的提高。经强化絮凝、增设斜管、折板等综合性改造后．单池产水量由原来80m^3／d增加到140m^3／d,药耗降低了15％～20％。改造后仅1年多时间．即可从增产水的效益和节省药耗中收回投资。