Study on Wusan Granule Anti-tumor Related Target Gene Screened by cDNA Microarray

Wusan Granule, the Ⅲ new drug, is composed of such traditional Chinese medicines as Radix Polygorri Multiflori Praeparata, Radix Noginseng and the like. In 2001, State Drug Administration of China approved clinical trial for Wusan Granule. Wusan Granule possesses the superiority of safety and effectiveness. Its main functions are to supplement Qi and nourish Yin, and to dissipate mass. The animal experiment showing the recipe for Wusan Granule can not only suppress Lewis lung carcinom…     

激光微束穿刺法获得抗虫转基因油菜及其后代的研究

利用激光微束穿刺法将苏云金芽孢杆菌的毒蛋白 (δ endotoxin)基因导入了油菜 ,经聚合酶催化链式反应(PCR)和PCRSouthern杂交证明抗虫基因已导入油菜基因组中并能在T1 代得到遗传。对转基因植株进行了抗虫性测试 ,结果表明某些植株具有较好的抗虫性 ,并且这种抗虫性在T1 代仍能保持。实验结果表明抗虫基因已整合进油菜基因组并得到了稳定的遗传。     

Matrix Metalloproteinase Responsive,Proximity‐Activated Polymeric Nanoparticles for siRNA Delivery

Small interfering RNA (siRNA) has significant potential to evolve into a new class of pharmaceutical inhibitors, but technologies that enable robust, tissue‐specific intracellular delivery must be developed before effective clinical translation can be achieved. A pH‐responsive, smart polymeric nanoparticle (SPN) with matrix metalloproteinase (MMP)‐7‐dependent proximity‐activated targeting (PAT) is described here. The PAT‐SPN is designed to trigger cellular uptake and cytosolic delivery of siRNA once activated by MMP‐7, an enzyme whose overexpression is a hallmark of cancer initiation and progression. The PAT‐SPN is composed of a corona‐forming polyethylene glycol (PEG) block, an MMP‐7‐cleavable peptide, a cationic siRNA‐condensing block, and a pH‐responsive, endosomolytic terpolymer block that drives self‐assembly and forms the PAT‐SPN core. With this novel design, the PEG corona shields cellular interactions until it is cleaved in MMP‐7‐rich environments, shifting the SPN ζ‐potential from +5.8 to +14.4 mV and triggering a 2.5 fold increase in carrier internalization. The PAT‐SPN exhibits pH‐dependent membrane disruptive behavior that enables siRNA escape from endo‐lysosomal pathways. Intracellular siRNA delivery and knockdown of the model enzyme luciferase in R221A‐Luc mammary tumor cells is significantly increased by MMP‐7 pre‐activation (p < 0.05). These combined data indicate that the PAT‐SPN provides a promising new platform for tissue‐specific, proximity‐activated siRNA delivery to MMP‐rich pathological environments.     

壬基酚对大鼠F1子代脑基因表达的影响

目的:研究壬基酚通过妊娠期暴露是否会影响F1子代的脑发育。方法:对SD孕鼠染毒NP,从妊娠第7天开始持续到产后第二天,分别提取NP组和对照组F1代2d龄大鼠的脑组织mRNA,表达谱基因芯片检测脑基因的表达。用Q-PCR法验证F1雄性子代大脑泛素特异性蛋白酶8、ATP结合盒转运子和内磺肽蛋白这三个下调基因的表达,然后用Western blotting法检测F1子代大脑泛素特异性蛋白酶8的蛋白表达。结果:ATP结合盒转运子(ATP-binding cassette,ABCA)、内璜肽蛋白(Endosulfine alph,Ensa)、泛素特异性蛋白酶8(Ubiquitin Specific Protease 8,USP8/UBPy)基因和蛋白表达下调。结论:NP可影响神经细胞的正常结构和信号转导功能,并提示可以干扰机体内分泌系统、脂质和能量代谢及一系列正常生理功能。     

Managing the Public Sphere: Journalistic Construction of the Great Globalization Debate

There is little consensus on what constitutes open, deliberative media discourse. We offer a simple, measurable, and comparative model based on 3 aspects of source and issue construction in news accounts: access, recognition, and responsiveness. The model is applied to coverage of 2001–2003 World Economic Forum (WEF) meetings and protests against the organization's role in global economic policies. Both demonstrators and WEF participants were granted news access, but WEF actors were recognized more formally and given greater input in news content, including ownership claims to many activist issue positions. Journalistic deference to the WEF communication agenda limited mutual responsiveness. The journalistic process systematically managed the debate about globalization on terms that favored elites over citizen-activists.     

Inorganic Nanomaterial-Mediated Gene Therapy in Combination with Other Antitumor Treatment Modalities

Cancer is a genetic disease originating from the accumulation of gene mutations in a cellular subpopulation. Although many therapeutic approaches have been developed to treat cancer, recent studies have revealed an irrefutable challenge that tumors evolve defenses against some therapies. Gene therapy may prove to be the ultimate panacea for cancer by correcting the fundamental genetic errors in tumors. The engineering of nanoscale inorganic carriers of cancer therapeutics has shown promising results in the efficacious and safe delivery of nucleic acids to treat oncological diseases in small-animal models. When these nanocarriers are used for co-delivery of gene therapeutics along with auxiliary treatments, the synergistic combination of therapies often leads to an amplified health benefit. In this review, an overview of the inorganic nanomaterials developed for combinatorial therapies of gene and other treatment modalities is presented. First, the main principles of using nucleic acids as therapeutics, inorganic nanocarriers for medical applications and delivery of gene/drug payloads are introduced. Next, the utility of recently developed inorganic nanomaterials in different combinations of gene therapy with each of chemo, immune, hyperthermal, and radio therapy is examined. Finally, current challenges in the clinical translation of inorganic nanomaterial-mediated therapies are presented and outlooks for the field are provided.     

超声微泡对pEGFP质粒转染小鼠损伤面神经的促进作用

目的：探讨超声微泡介导基因转染面神经的可行性及有效性。方法：建立小鼠面神经损伤模型,以增强型绿色荧光蛋白质粒（pEGFP）为标记基因。将48只小鼠随机分为6组：空白组（A组）、脂质体＋质粒组（B组）、微泡＋质粒＋超声组（C组）、微泡＋质粒组（D组）、超声＋质粒组（E组）、单纯质粒组（F组）。将小鼠面神经损伤后,局部注射...     

Redox‐Responsive and Drug‐Embedded Silica Nanoparticles with Unique Self‐Destruction Features for Efficient Gene/Drug Codelivery

The development of advanced gene/drug codelivery carriers with stimuli‐responsive release manner for complementary cancer therapy is desirable. In this study, novel disulfide‐bridged and doxorubicin (DOX)‐embedded degradable silica nanoparticles (DS‐DOX) with unique self‐destruction features are synthesized by a facile one‐pot method. In order to realize codelivery of genes and drugs, the surface of DS‐DOX nanoparticles is readily functionalized with the assembled polycation (CD‐PGEA), comprising one β‐cyclodextrin core and two ethanolamine‐functionalized poly(glycidyl methacrylate) arms, to achieve DS‐DOX‐PGEA. The redox‐responsive self‐destruction behavior of DS‐DOX imparts DS‐DOX‐PGEA with a better ability to release anticancer drug DOX, while the low‐toxic hydroxyl‐rich CD‐PGEA brushes can efficiently deliver genes for cancer treatment. Very interestingly, the degradation process of DS‐DOX starts from the outside, while the destruction of the degradable silica (DS) nanoparticles without DOX begins from the center of the nanoparticles. The embedded DOX inside the DS‐DOX nanoparticles can significantly influence the structures and facilitate the cellular uptake and the subsequent gene transfection. The as‐developed DS‐DOX‐PGEA nanostructure with coordinating biodegradability, stimuli‐responsiveness, and controlled release manner might be desirable gene/drug codelivery carriers for clinical cancer treatment.