The Effect of High and Variable Glucose on the Viability of Endothelial Cells Co-Cultured with Smooth Muscle Cells

Diabetes mellitus causes endothelial dysfunction. The aim of this study was to investigate the effect of normal (5 mmol/L), high (20 mmol/L), and fluctuating (5 and 20 mmol/L changed every day) glucose concentration in the culture medium on the viability of human umbilical vein endothelial cells (HUVECs) co-cultured with human umbilical artery smooth muscle cells (HUASMCs). The cultures were conducted on semi-permeable flat polysulfone (PSU) fibronectin-coated membranes immobilized in self-made inserts. The insert contained either HUVECs on a single membrane or HUASMCs and HUVECs on two membranes close to each other. Cultures were conducted for 7 or 14 days. Apoptosis, mitochondrial potential, and the production of reactive oxygen species and lactate by HUVECs were investigated. The results indicate that fluctuations in glucose concentration have a stronger negative effect on HUVECs viability than constant high glucose concentration. High and fluctuating glucose concentrations slow down cell proliferation compared to the culture carried out in the medium with normal glucose concentration. In conclusion, HUASMCs affect the viability of HUVECs when both types of cells are co-cultured in medium with normal or variable glucose concentration.     

Possible Role of GnIH as a Novel Link between Hyperphagia-Induced Obesity-Related Metabolic Derangements and Hypogonadism in Male Mice

Gonadotropin-inhibitory hormone (GnIH) is a reproductive inhibitor and an endogenous orexigenic neuropeptide that may be involved in energy homeostasis and reproduction. However, whether GnIH is a molecular signal link of metabolism and the reproductive system, and thus, regulates reproductive activity as a function of the energy state, is still unknown. In the present study, we investigated the involvement of GnIH in glycolipid metabolism and reproduction in vivo, and in the coupling between these two processes in the testis level. Our results showed that chronic intraperitoneal injection of GnIH into male mice not only increased food intake and altered meal microstructure but also significantly elevated body mass due to the increased mass of liver and epididymal white adipose tissue (eWAT), despite the loss of testicular weight. Furthermore, chronic intraperitoneal administration of GnIH to male mice resulted in obesity-related glycolipid metabolic derangements, showing hyperlipidemia, hyperglycemia, glucose intolerance, and insulin resistance through changes in the expression of glucose and lipid metabolism-related genes in the pancreas and eWAT, respectively. Interestingly, the expression of GnIH and GPR147 was markedly increased in the testis of mice under conditions of energy imbalance, such as fasting, acute hypoglycemia, and hyperglycemia. In addition, chronic GnIH injection markedly inhibited glucose and lipid metabolism of mice testis while significantly decreasing testosterone synthesis and sperm quality, inducing hypogonadism. These observations indicated that orexigenic GnIH triggers hyperphagia-induced obesity-related metabolic derangements and hypogonadism in male mice, suggesting that GnIH is an emerging candidate for coupling metabolism and fertility by involvement in obesity and metabolic disorder-induced reproductive dysfunction of the testes.     

miR-199a: A Tumor Suppressor with Noncoding RNA Network and Therapeutic Candidate in Lung Cancer

Lung cancer is the leading cause of cancer death worldwide. miR-199a, which has two mature molecules: miR-199a-3p and miR-199a-5p, plays an important biological role in the genesis and development of tumors. We collected recent research results on lung cancer and miR-199a from Google Scholar and PubMed databases. The biological functions of miR-199a in lung cancer are reviewed in detail, and its potential roles in lung cancer diagnosis and treatment are discussed. With miR-199a as the core point and a divergence outward, the interplay between miR-199a and other ncRNAs is reviewed, and a regulatory network covering various cancers is depicted, which can help us to better understand the mechanism of cancer occurrence and provide a means for developing novel therapeutic strategies. In addition, the current methods of diagnosis and treatment of lung cancer are reviewed. Finally, a conclusion was drawn: miR-199a inhibits the development of lung cancer, especially by inhibiting the proliferation, infiltration, and migration of lung cancer cells, inhibiting tumor angiogenesis, increasing the apoptosis of lung cancer cells, and affecting the drug resistance of lung cancer cells. This review aims to provide new insights into lung cancer therapy and prevention.     

液相法制备纳米钯粉的工艺研究

采用液相法,以Pd粉为原料,分别用HCHO,HCOOM,C2H4H2O作还原剂,把钯还原成纳米粉末。通过控制反应体系的pH值、浓度、温度及还原剂的种类和还原时间来控制粉末的粒径大小及其分布.实验结果表明:利用本法制备的Pd粉粒径在9 nm~20 nm,粒度分布均匀而且重现性好,适用于制造钯银导体浆料.     

~(19)F(p,αγ)~(16)O瞬发核反应测定茶叶中的含氟量

本文介绍了在400kV高压倍加器上,利用~(19)F(p,αγ)~(16)O瞬发核反应测量国内150余种茶叶样品含氟量的原理及实验装置,给出了一批典型的测量结果。结果表明:同一品种的茶叶,茶的品级数越大,含氟量越高;茶叶的品种越好,含氟量越低。     

选矿浓缩过程矿浆浓度的模糊控制

针对治金行业选矿浓缩过程的工艺特点,介绍了基于变频技术的矿浆浓度模糊控制,较好地控制了浓度指标,同时也稳定了矿浆流量并大幅度节能降耗。该设计已在宝钢、武钢等选矿厂中得到成功应用,效益显著。     

厚壁容器圆柱壳开孔应力集中问题(Ⅱ)

根据厚壁圆筒形压力容器开孔应力集中系数的一般表达式，利用正交函数展开的方法，将厚壁圆柱壳的开孔应力问题简化为求解一组无穷代数方程组问题为说明复变函数分析方法的适用性，给出了圆筒形压力容器在自由边界条件下，开圆孔和椭圆孔应力集中系数的数值结果     

CAP1400控制棒提升极限分析方法研究

控制棒提升极限用于限定控制棒组棒位和可溶硼浓度的范围,以防止慢化剂温度系数突破限值。CAP1400核电厂采用机械补偿运行策略,使控制棒及硼浓度运行范围大为扩展,同时功能独立的M棒组和AO棒组同时插入堆芯使得插棒情况更为复杂,因此与传统核电厂相比,CAP1400核电厂的控制棒提升极限更难界定。本文建立了适用于CAP1400核电厂的控制棒提升极限分析方法,并给出计算结果。本文提出的方法合理地解决了复杂的控制棒运行情况给提升极限造成的影响,并充分地利用了电厂实测数据对提升极限进行修正。基于本文方法得到的提升极限精确且具备一定的保守性,所以便于电厂实际运行时使用。     

A Remark to a Formula in K. Ishimatsu's Paper on “Continuous γ-ray Spectrometry with Scintillation Counters”

Spatial distribution of the Cd-ratio of Au foils were measured for various lattice pitches in a natural uranium-light water system. Comparison of the experimental results with theoretical calculation using the THERMOS-code shows some divergence of the theoretical Cd-ratio toward larger than experimental values in the fuel rod.     

0.5—7.5MeV高能磷离子注入硅中剖面分布及剖面迭加

本文对磷离子能量在0.5—7.5MeV、剂量在2.0×10~(12)—1.0×10~(13)/cm~2范围内注入硅中的载流子剖面进行了研究,发现在此条件下,n型磷载流子剖面是由两个只有不同偏差σ_(tront)(σ前)和σ_(back)(σ后)的高斯分布相连接组成的。根据实验所得剖面特性,提出了多次迭加形成平坦剖面的方法。用这种方法结合SiO_2膜屏蔽和调整注入离子能量、剂量,能够获得各种有特殊要求的载流子浓度剖面。为了得到好的可控性和可重复性,采用了瞬态退火技术。透射电镜分析表明:在我们所用退火条件下,剩余缺陷并未出现。这种迭加注入已应用于特种器件研制中,试制出的产品获得令人满意的结果。