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BACKGROUND: Theabrownin (TB) is a main bioactive component in Pu‐erh tea, and the total amount is between 100 and 140 g kg?1. However, reports on the mechanism of formation of TB are sparse because it has a high molecular weight and complex composition. Hence, the mechanism of formation of TB in Pu‐erh tea during solid state fermentation was investigated using an exogenous enzyme method. RESULTS: It was found that, in the presence of exogenous enzymes, the tea liquor prepared from the resulting leaves changed considerably in colour. In addition, the TB, total carbohydrate, polysaccharide, amino acid and protein contents were all increased, while the tea polyphenol content decreased sharply; the surfaces of leaves before fermentation appeared to be smooth and intact, and the structures of the cell, cellulose and lignin were complete, while after fermentation their surfaces were covered by microorganisms and the structures of the cells were largely disrupted. CONCLUSION: The enzymatic actions are closely related to the compositional changes occurring during Pu‐erh tea manufacture, and its quality. Enzymes produced by microorganisms were found to be the main cause of TB formation during the fermentation of Pu‐erh tea. Copyright © 2011 Society of Chemical Industry 相似文献
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Toshio Hasegawa Takashi Fujita Yasutsugu Tsukumo Takashi Fujihara Daisuke Jing Atsushi Takahashi Kenta Nakajima 《食品科学与工程:英文版(2...》2014,(1):10-20
Although many aroma components have been identified in green tea leaves, the aroma compounds contributing to green tea's characteristic odor have not yet been reported. The authors recently reported that aroma components with a matcha-like odor are present in both green tea and black tea prepared from the Sayamakaori tea cuttivar. This matcha-like odor is similar to the odor of commercial available matcha (high-quality powdered green tea), and is a specific odor feature of green tea leaves. At present, the green-tea odor is thought to arise from the combination of a large number of constituents. Recent reports indicate that a complex interaction between olfactory receptors and odorants is important for the evaluation of the odors. Taking into consideration these findings, the authors investigated the aroma profile of green tea, focusing on the characteristic molecular structures of the constituents that give matcha-like odor. Using a combination of organic synthesis and gas chromatography-mass spectrometry plus gas chromatography-olfactometry, the authors identified aroma components with matcha-like odors in five other tea cultivars. This investigation also revealed that several compounds with a formyl group were important constituents of the aroma of green tea leaves, although the odor of each constituent was not individually similar to the tea's overall aroma. The authors found for the first time a group of key components that have the matcha-like odor. 相似文献
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三波长分光光度法测定红茶及其饮料中黄酮的含量 总被引:4,自引:1,他引:4
采用三波长分光光度法测定红茶及其饮料中黄酮的含量,消除了由于红茶中黄酮光谱吸收峰不对称给定量分析造成的影响,并校正了基于干扰组分(饮料中添加剂)的吸收光谱具有线性吸收产生的基线倾斜。实验结果,回归方程为:DA=-3.221?0-4 0.06735C,相关系数r=0.9992;黄酮的浓度在1000~3000g/mL范围内,分别在波长为l1=470nm、l2=420nm、l3=370nm处测吸光度时,则DA与浓度C之间呈良好的线性关系,可按标准曲线法进行定量分析。本法的回收率为96.0%~107.0%,变异系数小于0.48%。方法的准确度与精密度均令人满意,而且操作简便易行。 相似文献
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以羊栖菜为原料,通过对羊栖菜干制技术及调味配方的研究,制成一种方便营养的鸡肉味即食羊栖菜调味包。实验结果表明,干燥羊栖菜的最佳工艺为:在95℃下漂烫90s后热风干燥70℃下3.5小时,脱水羊栖菜的复水形态、色泽等质量较好。最佳菜包配方为脱水红胡萝卜1.5g、脱水羊栖菜6g、脱水香葱0.6g;最佳调味粉配方为:4.5g、I+G0.03g、味精0.5g、大蒜粉0.125 g、生姜粉0.1 g、葱粉0.15 g、白胡椒粉0.1 g;最佳鸡汤味酱包为:棕榈油在3.5 g、食盐1.0 g、酱油1.6 g、鸡骨油1.5 g。 相似文献