Yeast exo-β-glucanases can be used as efficient and readily detectable reporter genes in Saccharomyces cerevisiae

Yeast exo-1,3-β-glucanases are secretable proteins whose function is basically trophic and may also be involved in cell wall glucan hydrolytic processes. Since fluorescein di(β-D -glucopyranoside) is a fluorogenic substrate detectable and quantifiable by flow cytometry, it was used for testing the ability of the EXG1 gene product of Saccharomyces cerevisiae and its homologous gene in Candida albicans to function as reporter genes. These open reading frames were coupled to different promoters in multicopy plasmids, and exoglucanase activity quantified at flow cytometry. Exoglucanases were found to be useful tools for the study of promoter regions in S. cerevisiae. This technique has the advantage over other reporter gene systems—such as β-galactosidase fusions—that it does not require permeabilization of yeast cells and therefore it allows the recovery of viable cells—by sorting—after flow cytometry analysis.     

气液两相流流量的互相关测量

在内径为３０ｍｍ的垂直上升管中，应用互相关分析和Ｕ型光导纤维探针，对水一气两相流和油－气两相流进行了各分相流量测量的实验研究，考虑了流型和雷诺数对流量测量的影响，同时研究了含气率的测量，提出了计算平均含气率的关系式。对水－气两相流，试验的气相折算流速范围为０．２２～３．００ｍ／ｓ，波相折算流速范围为０．１１～３．００ｍ／ｓ；对油一气两相流，试验的气相折算流速范围为０．１０～１９．０１ｍ／ｓ，液相折算流速范围为０．３５～１．６５ｍ／ｓ。     

ATM网络中漏桶流量控制特性的研究

本文对ＡＴＭ（异步转移模式）网络中话音、数据和图像业务的漏桶流量控制特性进行了研究。计算机模拟结果表明，由于各种业务内在的相关性不同，相应的漏桶流量控制特性有较大的差异。漏桶参数的选择对于各种业务具有不同的公平性，因而应根据业务的特性来设计漏桶参数。     

Effects of shear flow on long-ranged correlations,spinodal demixing kinetics,and the location of the critical point and cloud point

A derivation of an expression for the shear rate-dependent Ornstein Zernike structure factor is discussed, together with the resulting anomalous behavior of the turbidity. The predicted scaling behavior of the turbidity, comprising the effect of both temperature and shear rate, is in good agreement with experiments on binary fluids. Then initial spinodal decomposition is discussed, and an explicit expression for the time- and shear rate-dependent effective diffusion coefficient is derived, which shows all the typical characteristics of anisotropic light scattering patterns that are observed experimentally for binary fluids. Next it is shown that the spinodal is shifted linearly with the (bare) Peclet number for not too large Peclet numbers, whereas the cloud point is singularly displaced into the unstable region due to a shear now. This is in agreement with an experiment on a two-polymer/solvent mixture and binary fluid mixtures. It is argued that light scattering is useless to determine the location of the spinodal of a sheared system.Paper presented at the Twelfth Symposium on Thermophysical Properties, June 19–24, 1994, Boulder, Colorado, U.S.A.     

Characterization of the flow behaviour of sausage emulsions with a simple tube viscometer

A specially designed tube viscometer was used to measure apparent viscosity during flow of Bologna type sausage emulsions moving through a pipe. Emulsions varied in fat content (from 21.8 to 44.3%) and in moisture/protein ratio (from 3.7 to 5.6), and the effects of added sodium chloride, sodium diphosphate, starch and blood plasma were investigated in a 36% fat, 3.7 moisture/protein emulsion.
The shear stresses determined as a function of shear rate were fitted by a power law. Yield stress of the emulsions was negligible relative to the applied stress. However, inspection of the flow profiles indicated that considerable slip of the sausage emulsion occurred at the pipe wall. Both emulsion flow and slip contribute to overall flow behaviour, so a kind of effective viscosity is determined. Intrinsic rheological properties and wall slip will both be affected by the composition of the sausage emulsion.     

Simulation of water and nitrogen dynamics in soils during wastewater applications by using a finite-element model

A mathematical model was developed to simulate water movement, mass transport, and nitrogen transformations in soils during wastewater applications. The model is one-dimensional and based on the Galerkin finite-element method. The submodel of mass transport of nitrogen incorporates the convection-dispersion processes of ammonium and nitrate nitrogen, nitrification, denitrification, ammonium exchange and uptake of ammonium and nitrate ions. The accuracy and validity of the proposed model was examined by comparison with an explicit-implicit finite-difference model results. The model was used for simulation of water and nitrogen dynamics during wastewater application in homogeneous and multi-layered soils under different N concentration, rate, duration and scheduling of application.     

FINITE ELEMENT ANALYSIS OF THE FLOW INDUCED BY ROTATING BLADES IN AN INCOMPRESSIBLE VISCOUS FLUID

Aerodynamic loads on a multi-bladed helicopter rotor in hovering flight were calculated by solving the three-dimensional incompressible Navier-Stokes equations. The rotor wake effects were accounted by the correction of local geometric angle of attack according to a free-wake modeling in addition to an empirical modification for the tip flow effect. The validity and efficiency of the present method were verified by the comparisons between numerical results and experimental data.     

Influence of Coherent Flow Structures on the Dynamics of Suspended Sediment Transport in Open-Channel Flow

The influence of suspended sediments on coherent flow structures has been studied by simultaneously measuring the longitudinal and vertical components of the instantaneous velocity vector and the instantaneous suspended particle concentration with an acoustic particle flux profiler. The measurements were carried out in clear water and in particle-laden open-channel flows. In both cases, they clearly show the predominance of ejection and sweep phases that are part of a burst cycle. The analysis further demonstrates the importance of the ejection and sweep phases in sediment resuspension and transport. Ejections pick up the sediment at the bed and carry it up through the water column close to the surface. It is shown that ejections and sweeps are in near equality in the near-bottom layer, whereas ejections clearly dominate in the remaining water column. The implications of these results for sediment transport dynamics are discussed.     

Discrete Modeling and Suggested Measurement of Heat Transfer in Gas-Solids Flows

This article presents a two-dimensional transient model for gas-solids flow and heat transfer through pipes using the coupled Computational Fluid Dynamics and Discrete Element Method approach. Numerical simulations have been conducted to examine the modification of fluid thermal structure due to the presence of particles in a pneumatic transport pipeline. Modeled results have demonstrated the key role of transversal motion of rebounding particles in the pipe cross section in altering fluid temperature. Further implementation of this modeling technique in air-drying processes is discussed and possible experimental methods for the measurement of in situ particle and fluid motion and temperature profile are cited.     

Mathematical analysis of viscometric (polymer) flow fields in capillaries: Taylor dispersion revisited

A general formulation for capillary flow of two miscible fluids – one a dilute plug of polymer fluid inserted into a fully developed Poiseuille flow of the other, a Newtonian stream – is examined for its long time behavior. Phenomenologically, the system evolves from an initial state, that of a plug within the boundaries of sharp, well defined fronts inside a Newtonian stream, to a more homogenized state in the very long time scale. This problem was addressed by G.I. Taylor but with regard to a system of two Newtonian fluids, leading to the well-known results commonly described as `Taylor axial dispersion'. In this paper, a general and systematic perturbation analysis is presented from which Taylor's result is recovered as a special case of a more general solution which applies to fluids incorporating elastic properties. In particular, the influence of viscoelasticity and (polymer) diffusivity on the observed pressure profile in the capillary conduit is examined. This effect is clearly separated out for small Peclet number flows using asymptotic and numerical analysis. The results identify the influence of fluid viscosity, elasticity, and diffusivity on the observed pressure profile and form the basis for the improved characterization of polymeric elasticity using capillaries – a finding that is of significant scientific and commercial interest. These results were obtained by the authors as a class of observations resulting from the perturbation analysis of forced-flow capillary devices in viscoelastic fluid property investigation.