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Yong Pan Chen Zhu Wen Bin Zeng Pei Fu Chi Chen Bao Ming Xu Zhong Feng Gao 《Chembiochem : a European journal of chemical biology》2021,22(24):3431-3436
Rapid and sensitive point-of-care testing (POCT) is an extremely critical mission in practical applications, especially for rigorous military medicine, home health care, and in the third world. Here, we report a visual POCT method for adenosine triphosphate (ATP) detection based on Taylor rising in the corner of quadratic geometries between two rod surfaces. We discuss the principle of Taylor rising, demonstrating that it is significantly influenced by contact angle, surface tension, and density of the sample, which are controlled by ATP-dependent rolling circle amplification (RCA). In the presence of ATP, RCA reaction effectively suppresses Taylor-rising behavior, due to the increased contact angle, density, and decreased surface tension. Without addition of ATP, untriggered RCA reaction is favorable for Taylor rising, resulting in a significant height. With this proposed method, visual sensitive detection of ATP without the aid of other instruments is realized with only a 5 μL droplet, which has good selectivity and a low detection limit (17 nM). Importantly, this visual method provides a promising POCT tool for user-friendly molecular diagnostics. 相似文献
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Multicellular tumor spheroid models (MCTS) are often coined as 3D in vitro models that can mimic the microenvironment of tissues. MCTS have gained increasing interest in the nano‐biotechnology field as they can provide easily accessible information on the performance of nanoparticles without using animal models. Considering that many countries have put restrictions on animals testing, which will only tighten in the future as seen by the recent developments in the Netherlands, 3D models will become an even more valuable tool. Here, an overview on MCTS is provided, focusing on their use in cancer research as most nanoparticles are tested in MCTS for treatment of primary tumors. Thereafter, various types of nanoparticles—from self‐assembled block copolymers to inorganic nanoparticles, are discussed. A range of physicochemical parameters including the size, shape, surface chemistry, ligands attachment, stability, and stiffness are found to influence nanoparticles in MCTS. Some of these studies are complemented by animal studies confirming that lessons from MCTS can in part predict the behaviour in vivo. In summary, MCTS are suitable models to gain additional information on nanoparticles. While not being able to replace in vivo studies, they can bridge the gap between traditional 2D in vitro studies and in vivo models. 相似文献
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Finite element piezothermoelasticity analysis and the active control of FGM plates with integrated piezoelectric sensors and actuators 总被引:3,自引:0,他引:3
An efficient finite element model is presented for the static and dynamic piezothermoelastic analysis and control of FGM
plates under temperature gradient environments using integrated piezoelectric sensor/actuator layers. The properties of an FGM plate are functionally graded in the thickness
direction according to a volume fraction power law distribution. A constant displacement-cum-velocity feedback control algorithm that couples the direct and inverse piezoelectric effects is applied to provide active feedback
control of the integrated FGM plate in a closed loop system. Numerical results for the static and dynamic control are presented
for the FGM plate, which consists of zirconia and aluminum. The effects of the constituent volume fractions and the influence
of feedback control gain on the static and dynamic responses of the FGM plates are examined.
Received: 13 March 2002 / Accepted: 5 March 2003
The work described in this paper was supported by a grant awarded by the Research Grants Council of the Hong Kong Special
Administrative Region, China (Project No. CityU 1024/01E). 相似文献
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一种新颖的表面等离子谐振效应光波导水质传感技术 总被引:4,自引:0,他引:4
光学表面等离子谐振(SPR)效应是一种很有效的水质检测技术,经过详细的理论推导,同了SPR反射光强的普遍适用公式。在纯水与受污染水两种情况下,根据一些已知参数计算出结果。由计算结果数据一出的曲线比较表明,SPR效应在两种情况下有明显差别,这也正是SPR效应用于水质检测的理论根据。 相似文献
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氢电弧等离子体法制备的纳米镍铈粒子的催化特性 总被引:3,自引:0,他引:3
利用氢电弧等离子法制备具有储氢特性纳米镍铈粒子,并在气相苯加氢反应中研究了其催化特性,发现其催化活性与储氢特性及表面层中NiCe合金存在有关,且具有高的选择性和热稳定性,这与纳米粒子独特的结构及铈的加入有关。 相似文献
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