IMP2, a nuclear gene controlling the mitochondrial dependence of galactose, maltose and raffinose utilization in Saccharomyces cerevisiae.

The IMP2 gene of Saccharomyces cerevisiae is involved in the nucleo-mitochondrial control of maltose, galactose and raffinose utilization as shown by the inability of imp2 mutants to grow on these carbon sources in respiratory-deficient conditions or in the presence of ethidium bromide and erythromycin. The negative phenotype cannot be scored in the presence of inhibitors of respiration and oxidative phosphorylation, indicating that the role of the mitochondria in the utilization of the above-mentioned carbon sources in imp2 mutants is not at the energetical level. Mutations in the IMP2 gene also confer many phenotypic alterations in respiratory-sufficient conditions, e.g. leaky phenotype on oxidizable carbon sources, sensitivity to heat shock and sporulation deficiency. The IMP2 gene has been cloned, sequenced and disrupted. The phenotype of null imp2 mutants is indistinguishable from that of the originally isolated mutant.     

抗生素生产过程补料时机的Fuzzy识别与预报

本文就抗生素发酵生产过程,提出了一种补料时机的Fuzzy识别、预报的建模方法,并根据20m~3发酵罐工业数据的分析,选取了以总糖、总糖变化率、氨基氮和氨基氮变化率四个主要参数作为输入集因素,运用本文提出的方法,建立了一个实际的抗生素发酵生产过程补料时机的 Fuzzy 识别、预报模型。经现场调试效果良好,与富有经验的行家相比,准确率达90%以上。     

On the design of multiple key hashing files for concurrent orthogonal range retrieval between two disks

This paper concerns the following problem: given a set of multi-attribute records, a fixed number of buckets and a two-disk system, arrange the records into the buckets and then store the buckets between the disks in such a way that, over all possible orthogonal range queries (ORQs), the disk access concurrency is maximized. We shall adopt the multiple key hashing (MKH) method for arranging records into buckets and use the disk modulo (DM) allocation method for storing buckets onto disks. Since the DM allocation method has been shown to be superior to any other allocation methods for allocating an MKH file onto a two-disk system for answering ORQs, the real issue is knowing how to determine an optimal way for organizing the records into buckets based upon the MKH concept.

A performance formula that can be used to evaluate the average response time, over all possible ORQs, of an MKH file in a two-disk system using the DM allocation method is first presented. Based upon this formula, it is shown that our design problem is related to a notoriously difficult problem, namely the Prime Number Problem. Then a performance lower bound and an efficient algorithm for designing optimal MKH files in certain cases are presented. It is pointed out that in some cases the optimal MKH file for ORQs in a two-disk system using the DM allocation method is identical to the optimal MKH file for ORQs in a single-disk system and the optimal average response time in a two-disk system is slightly greater than one half of that in a single-disk system.     

柱体自由扭转时的最优横截面形式

当柱体自由扭转时，柱体的抗扭性能与横截面形式有关，针对单连通域下的具有最优抗扭性能的横截面形式问题，本文建立了相应的最优形式控制模型，并通过变分法求解得到：在横截面面积相等的情况下，圆截面是所有截面中使结构整体应变能最小和扭转刚度最大的截面形式，这与圣维南所给的一般性结论是一致的。     

反应器网络综合优化方法的研究进展

反应器网络综合优化方法在选择反应器类型和反应工艺条件时,比传统的方法表现出了很大的优势。综述了各类反应器网络综合优化方法的基本原理和研究进展,并分析了它们的优缺点。这些方法有基于过程特征的方法,包括可得区法和导数分析法;超结构优化法;目标类方法,包括目标法、构造目标法和构造MINLP法;以及经验推断法和分布参数法等。最后分析了这方面研究的发展趋势。     

山区水库溢洪道和放水洞工程布置优化组合及型式选择

山区河流上修水库与平原水库不同的是，地形复杂、地质条件各异。要充分利用坝址处的地物地貌及地质条件，如何趋利避害，合理布置水库的土坝走向，放水洞与溢洪道的位置及型式，以取得安全、经济、美观三者协调统一。     

昆2井优快钻井及油气层保护技术

昆2井是一口位于柴北缘西段的昆特依凹陷潜伏Ⅰ号圈闭上的风险探井,设计井深为5950m。针对柴北缘深探井存在的主要技术难题,制定了昆2井的技术对策。通过对钻井设备的优选、井身结构的优化、钻头及钻具组合的优选、钻井参数及钻井液性能的优化、油气层保护技术的研究与应用,形成了一套适合于柴北缘的优快钻井技术,使昆2井的完钻井深达到5950m。与邻区的深探井平均水平相比,在井深增加888m、大尺寸井眼段更长的前提下,昆2井的钻井、完井周期分别缩短了98.75d、124.2d,钻机月速度增加了317m/台,平均机械钻速增加了0.82m/h。根据“理想充填新理论”,确定了保护油气层的最优复配暂堵方案,利用该方案所配制的钻井液其渗透率恢复值达到了87.5%以上,流变性能良好,滤失量低,对改善泥饼质量、增强井壁稳定性有明显的效果。     

Application of artificial neural networks to the analysis of two‐dimensional fluorescence spectra in recombinant E coli fermentation processes

A two‐dimensional (2D) spectrofluorometer was used to monitor various fermentation processes with recombinant E coli for the production of 5‐aminolevulinic acid (ALA). The whole fluorescence spectral data obtained during a process were analyzed using artificial neural networks, ie self‐organizing map (SOM) and feedforward backpropagation neural network (BPNN). The SOM‐based classification of the whole spectral data has made it possible to qualitatively associate some process parameters with the normalized weights and variances, and to select some useful combinations of excitation and emission wavelengths. Based on the classified fluorescence spectra a supervised BPNN algorithm was used to predict some of the process parameters. It was also shown that the BPNN models could elucidate some sections of the process's performance, eg forecasting the process's performance. Copyright © 2005 Society of Chemical Industry     

天生桥梯级水电站联合调度决策支持系统建设经验

决策支持系统是水库优化调度实用化的发展方向，对于提高水电站生产效益和水库调度管理水平具有重要意义。结合国电公司重点科技项目“天生桥梯级水电站联合调度决策支持系统”的开发，阐述了开发水电调度决策支持系统实现水库调度实用化的经验:正确反映用户需求、增强用户对系统的控制、用模拟仿真弥补优化调度的不足、用数据库技术扩展水库优化调度实用化功能。     

Lactic acid production from dining‐hall food waste by Lactobacillus plantarum using response surface methodology

BACKGROUND: Food waste generally has a high starch content and is rich in nutritional compounds, including lipids and proteins. It therefore represents a potential renewable resource. In this study, dining‐hall food waste was used as a substrate for lactic acid production, and response surface methodology was employed to optimise the fermentation conditions. RESULTS: Lactic acid biosynthesis was significantly affected by the interaction of protease and temperature. Protease, temperature and CaCO₃ had significant linear effects on lactic acid production, while α‐amylase and yeast extract had insignificant effects. The optimal conditions were found to be an α‐amylase activity of 13.86 U g^?1 dried food waste, a protease activity of 2.12 U g^?1 dried food waste, a temperature of 29.31 °C and a CaCO₃ concentration of 62.67 g L^?1, which resulted in a maximum lactic acid concentration of 98.51 g L^?1 (88.75% yield). An increase in inoculum size would be appropriate for accelerating the depletion of initial soluble carbohydrate to enhance the efficiency of α‐amylase in dining‐hall food waste fermentation. CONCLUSION: A suitable regression model for lactic acid production was developed based on the experimental results. Dining‐hall food waste was found to be a good substrate for lactic acid fermentation with high product yield and without nutrient supplementation. Copyright © 2008 Society of Chemical Industry