人工髋关节置换术后发生医院感染的原因分析

目的 分析导致人工髋关节置换术后医院感染的原因,为临床预防及治疗提供有意义的借鉴.方法 回顾性分析我院2009年12月～2011年12月155例人工髋关节置换术患者发生医院感染的原因.结果 共发生医院感染10例,切口感染率6.5％,经采取及时有效处理措施,患者恢复良好.结论 严格消毒隔离及无菌技术,加强微生物监测及环境管理,及时治疗基础疾病、减轻术后疼痛、防止环境和空气污染是预防人工髋关节置换术后发生医院感染的有效措施.     

On the applicability of the laminar flow index when selecting surgical lighting

In modern operating rooms ultraclean air is supplied through the ceiling to prevent bacteria from entering an operating wound and cause infections. Operating lamps can disturb this flow of clean air. In this paper the accuracy of some aspects of the Laminar Flow Index of operating lamps was tested. The disturbance of the airflow was determined for different shapes and sizes of operating lamps in an isothermal situation. To accomplish this, an experimental study and a simulation study of a small room with an operating lamp were performed. The infection risk was found to be proportional to the projected surface area of the lamp. Although different lamp shapes resulted in a distinctly different flow pattern in the room, this did not affect the infection risk in a significant way. Operating lamps with a projected area smaller than 0.1 m² do not disturb the airflow at all in this particular setup.     

Associations between pathogen-specific cases of subclinical mastitis and milk yield,quality, protein composition,and cheese-making traits in dairy cows

The aim of this study was to investigate associations between pathogen-specific cases of subclinical mastitis and milk yield, quality, protein composition, and cheese-making traits. Forty-one multibreed herds were selected for the study, and composite milk samples were collected from 1,508 cows belonging to 3 specialized dairy breeds (Holstein Friesian, Brown Swiss, and Jersey) and 3 dual-purpose breeds of Alpine origin (Simmental, Rendena, and Grey Alpine). Milk composition [i.e., fat, protein, casein, lactose, pH, urea, and somatic cell count (SCC)] was analyzed, and separation of protein fractions was performed by reversed-phase high performance liquid chromatography. Eleven coagulation traits were measured: 5 traditional milk coagulation properties [time from rennet addition to milk gelation (RCT, min), curd-firming rate as the time to a curd firmness (CF) of 20 mm (k₂₀, min), and CF at 30, 45, and 60 min from rennet addition (a₃₀, a₄₅, and a₆₀, mm)], and 6 new curd firming and syneresis traits [potential asymptotical CF at an infinite time (CF_P, mm), curd-firming instant rate constant (k_CF, % × min^?1), curd syneresis instant rate constant (k_SR, % × min^?1), modeled RCT (RCT_eq, min), maximum CF value (CF_max, mm), and time at CF_max (t_max, min)]. We also measured 3 cheese yield traits, expressing the weights of total fresh curd (%CY_CURD), dry matter (%CY_SOLIDS), and water (%CY_WATER) in the curd as percentages of the weight of the processed milk, and 4 nutrient recovery traits (REC_PROTEIN, REC_FAT, REC_SOLIDS, and REC_ENERGY), representing the percentage ratio between each nutrient in the curd and milk. Milk samples with SCC > 100,000 cells/mL were subjected to bacteriological examination. All samples were divided into 7 clusters of udder health (UH) status: healthy (cows with milk SCC < 100,000 cells/mL and uncultured); culture-negative samples with low, medium, or high SCC; and culture-positive samples divided into contagious, environmental, and opportunistic intramammary infection (IMI). Data were analyzed using a linear mixed model. Significant variations in the casein to protein ratio and lactose content were observed in all culture-positive samples and in culture-negative samples with medium to high SCC compared to normal milk. No differences were observed among contagious, environmental, and opportunistic pathogens, suggesting an effect of inflammation rather than infection. The greatest impairment in milk quantity and composition, clotting ability, and cheese production was observed in the 2 UH status groups with the highest milk SCC (i.e., contagious IMI and culture-negative samples with high SCC), revealing a discrepancy between the bacteriological results and inflammatory status, and thus confirming the importance of SCC as an indicator of udder health and milk quality.     

A nuclear-encoded intein in the fungal pathogen Cryptococcus neoformans.

We have used comparative sequence analysis to identify an intein-like sequence (protein splicing element) present in Cryptococcus neoformans, a fungal pathogen of humans. The sequence encoding this element is present in the C. neoformans PRP8 gene, as an in-frame insertion relative to the PRP8 genes of other organisms. It contains sequences similar to those of the protein-splicing domains of two previously described yeast inteins (in Saccharomyces cerevisiae and Candida tropicalis), although it lacks any recognizable internal endonuclease domain. The Cryptococcus neoformans intein (Cne PRP8) is only the second to be found in a eukaryote nuclear genome; the previously described yeast inteins occur at the same site in the VMA gene homologues of S. cerevisiae and C. tropicalis. The host gene of the Cryptococcus intein, PRP8, encodes a highly conserved mRNA splicing protein found as part of the spliceosome. The Cne PRP8 intein may be a useful drug target in addressing the cryptococcal infections so prevalent in AIDS patients.     

沈阳市城区园林树木病虫害的调查

沈阳市城区园林树木病虫害种类及发生现状调查结果表明：病虫害在和平、沈和、皇姑、大东，铁西、东陵和于洪等地区分布普遍。共发现为害园林树木主要害虫37种，其中叶部害虫14种，枝干部害虫19种、根部害虫4种；主要病害15种，其中叶部病害8种，茎干部病害6种、根部病害1种。树木长期生长不良，栽培密植、树种间不合理配植，不遵守适地适树原则等是沈阳市区内园林树木发生病虫害的主要原因。     

医学领域中的新型生物传感器

生物传感器作为一项新技术已广泛地应用于人体生化指标和各种病原体的检测，糖尿病和缺血一再灌注损伤病人的监测以及空间生命科学的在线监视等医学领域，它以准确，灵敏、高效，快速，设备简便和成本低等优点，逐渐成为近年来医学诊断监测技术的热点之一，对近年来国内外应用中医学领域中的新型生物传感器进行综述。     

New modules for PCR-based gene targeting in Candida albicans: rapid and efficient gene targeting using 100 bp of flanking homology region

The use of PCR-based techniques for directed gene alterations has become a standard tool in Saccharomyces cerevisiae. In our efforts to increase the speed of functional analysis of Candida albicans genes, we constructed a modular system of plasmid vectors and successfully applied PCR-amplified functional analysis (FA)-cassettes in the transformation of C. albicans. These cassettes facilitate: (a) gene disruptions; (b) tagging of 3'-ends of genes with green fluorescent protein (GFP); and (c) replacements of endogenous promoters to achieve regulated expression. The modules consists of a core of three selectable marker genes, CaURA3, CaHIS1 and CaARG4. Modules for C-terminal GFP-tagging were generated by adding GFP-sequences flanked at the 5'-end by a (Gly-Ala)3-linker and at the 3'-end by the S. cerevisiae URA3-terminator to these selection markers. Promoter exchange modules consist of the respective marker genes followed by the regulatable CaMAL2 or CaMET3 promoters at their 3'-ends. In order to ensure a reliably high rate of homologous gene targeting, the flanking homology regions required a size of 100 bp of gene-specific sequences, which were provided with the oligonucleotide primers. The use of shorter flanking homology regions produced unsatisfactory results with C. albicans strain BWP17. With these new modules only a minimal set of primers is required to achieve the functional analysis of C. albicans genes and, therefore, provides a basic tool to increase the number of functionally characterized C. albicans genes of this human pathogen in the near future.     

Influence of pulsation rate on udder health and teat thickness changes in dairy ewes

In this work on machine milking of ewes, pulsation rates of 120 and 180 cycles per min were compared, both with a pulsation ratio of 50:50 and a vacuum level of 36 kPa, comparing intramammary infection (IMI), somatic cell count (SCC) and teat end thickness changes. To this end, two groups of 20 Manchega ewes were used in a crossover experimental design with two experimental periods of 24 d for each. Bacterial exposure of all teats was increased by dipping them in a suspension of Staphylococcus simulans at four consecutive milkings of each period. Pulsation rate of 180 cycles per min, compared with 120 cycles per min, had no negative effect upon new IMI (11 and 16% of ewes infected, respectively) and SCC. No teat end lesions were observed in those animals milked with the two pulsations assayed. Also, teat thickness changes (-0.38 and -0.36 mm at 120 and 180 cycles per min, respectively) were not affected significantly. Finally, in absence of IMI, the two pulsation rates assayed did not affect the SCC.     

论输血感染案件的损害赔偿责任

在输血感染案件中,血站所承担的民事责任性质应当是因缺陷产品致人损害的产品责任;就医疗机构的法律责任而言,存在着输血感染事故的责任竞合问题;对患者的损害赔偿包括患者及其近亲属的财产损失赔偿和精神损害赔偿。