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61.
Quantification of γ‐ and α‐tocopherol isomers in combination with pattern recognition model as a tool for differentiating dry‐cured shoulders of Iberian pigs raised on different feeding systems
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Eun Gyo Lee Seung Hui Lee Kyung Mi Park Jung Eun Baek Sun Hee Yeon Jin‐Ki Park Won‐Kyong Chang Joon‐Ki Jung Bong Hyun Chung 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2009,84(5):643-649
BACKGROUND: Human erythropoietin (hEPO), a hydrophobic acidic glycoprotein responsible for the regulation of red blood cell production in mammals, is used for the treatment of anemia. In general, the purification of transgenic animal‐derived therapeutic proteins is not easy due to their low titer concentrations and abundant contaminant proteins. For the first time, here the purification and characterization of rhEPO from the milk of transgenic pigs are described. RESULTS: The rhEPO was purified by heparin chromatography, reverse‐phase chromatography, and gel filtration chromatography, resulting in a 16.5% yield and > 98% purity. The rhEPO purified from the milk of transgenic pigs contained less acidic isoforms and was underglycosylated in contrast to CHO‐derived rhEPO. Cell proliferation of the F‐36/EPO‐dependent cell line was proportional to the dose of transgenic pig‐derived rhEPO. CONCLUSION: Transgenic pig‐derived rhEPO with high purity was achieved after three‐step chromatography following two‐step precipitation. The transgenic pig‐derived rhEPO was demonstrated to have comparable potency with CHO‐derived rhEPO. Transgenic pig‐derived rhEPO may not be therapeutically feasible because of different glycosylation, and thus further studies are required to elucidate the effect of this aberrant glycosylation on the biological activity and stability in vivo. Copyright © 2008 Society of Chemical Industry 相似文献
63.
Michael R. A. Morgan Ruth McNerney Henry W.-S. Chan Peter H. Anderson 《Journal of the science of food and agriculture》1986,37(5):475-480
An indirect, double antibody enzyme-linked immunosorbent assay (ELISA) has been validated for application to pig kidney. The specificity of the anti-ochratoxin A antiserum employed is such that minimal sample preparation is required prior to assay, with consequent beneficial effects on sample throughput. Kidneys (303) obtained in the UK as being unsuitable for human consumption were examined for ochratoxin A content by ELISA. Of these, 191 (63%) contained no detectable toxin (the detection limit was 0.5 ng g?1). Only eight samples (2.6%) contained more than 5 ng g?1 ochratoxin A, and only two were above 10 ng g?1 (at 11.5 and 12.4 ng g?1). The significance of these findings is discussed. 相似文献
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Vita Parra María Jesús Petrón Lourdes Martín Julio Manuel Broncano María Luisa Timón 《European Journal of Lipid Science and Technology》2010,112(7):720-726
The aim of this work was to study the influence of a diet with probiotic bacteria on the lipid composition of different tissues from Iberian pigs. Cholesterol and fatty acid profiles were measured in liver, subcutaneous fat, and Serratus ventralis muscle samples. Feeding Iberian pigs a mixture of probiotic microorganisms for 45 days prior to slaughter affected the tissue composition. The feeds of the control and probiotic groups were identical except for the added Bacillus licheniformis and Bacillus subtilis (1.28 × 109 cfu/kg feed) in the feed of the probiotic group. The diet with probiotics significantly affected the fatty acid profile of liver, subcutaneous fat and Serratus ventralis muscle samples, but no significant differences were found for the cholesterol content. The livers of the probiotic group contained lower levels of n‐6 fatty acids and higher levels of n‐3 fatty acids. The subcutaneous fat contained lower levels of n‐6 fatty acids. Serratus ventralis muscles from the probiotic group contained a significantly higher percentage of oleic acid than those of the control group. Practical applications: This study deals with the effect of the addition of probiotic bacteria to the diet of pigs on both the cholesterol and fatty acid content. B. subtilis and B. licheniformis can be used in pig feed (Regulation EC no. 2148/2004), but there are no studies about their effects on fresh meat or other tissues, particularly regarding the fat composition. Thus, it is interesting to study how feeding these microorganisms affects the different tissues. Including these microorganisms in the basic diet could serve as a strategy to modify the fat composition and to obtain healthier products from pigs. 相似文献
66.
天然植物提取物对生长猪的促生长作用及其内分泌机制的研究 总被引:8,自引:0,他引:8
以杜长大三元杂交生长猪为试验对象,研究了在饲粮中添加0.3%天然植物提取物对其的促生长作用及内分泌机制的影响。结果表明:(1)生长猪日增重提高6.90%(P<0.05),饲料转化率提高6.73%(P<0.05);(2)血清中总蛋白、球蛋白、总胆固醇和甘油三酯含量,分别比对照组提高了11.13%(P<0.05)、19.88%(P<0.05)、12.39%(P<0.05)和21.43%(P<0.05)。谷丙转氨酶、谷草转氨酶和碱性磷酸酶活性,分别比对照组提高了13.39%(P<0.05)、14.16%(P<0.05)和12.14%(P<0.05);(3)血清中生长激素、类胰岛生长因子分别比对照组提高了21.09%(P<0.05)和17.20%(P<0.05)。三碘甲腺原氨酸、甲状腺素和环腺苷酸含量,分别比对照组增加了16.01%(P<0.05)、17.06%(P<0.05)和19.88%(P<0.05)。 相似文献
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68.
复鞣剂在猪服装革上的配伍使用研究 总被引:1,自引:1,他引:1
本文对猪服装革生产中复鞣材料的搭配使用进行了研究。主要考查了当前国内市场上有代表性的几种复鞣材料,PR-1,ART-I,DM,HS以及KMC。通过单-材料实验确定各种复鞣材料性能,然后进行了这些材料的合理搭配实验,选择出了能体现皮革柔软,丰满,粒面细致,真皮感强,松面率低等流行风格的最佳搭配方案。 相似文献
69.
Eva Bauer Barbara A Williams Marlou W Bosch Christina Voigt Rainer Mosenthin Martin WA Verstegen 《Journal of the science of food and agriculture》2004,84(15):2097-2104
To determine whether faecal microorganisms can represent the entire large intestinal population, samples from caecum, mid‐colon and rectum of three adult pigs were used for the in vitro fermentation of fructo‐oligosaccharide (FOS), potato starch, wheat bran and oat hulls. The cumulative gas production technique measured fermentation kinetics and end‐products such as total gas, NH3 and volatile fatty acids (VFA). There were significant differences in the fermentability of substrates, in terms of both kinetics and end‐products. More relevant to this study, there were also differences between pigs in respect of total gas production, the rate of gas production (RM) and VFA production. For large intestine sections, there were more VFA from mid‐colon and rectal inocula compared with that from the caecum (p < 0.0001). Total gas, RM and NH3 were highest for rectal, intermediate for mid‐colon and lowest for caecal inocula (p < 0.0001). It was concluded that, while faecal sampling might overestimate caecal fermentation, its use is valid for in vitro assessment of large intestinal fermentation. However, differences between pigs indicate that a mix of samples from several animals remains important. Copyright © 2004 Society of Chemical Industry 相似文献
70.