哮喘儿童血清细胞因子水平及布拉氏酵母菌干预的研究

目的:探讨布拉氏酵母菌对儿童哮喘血清细胞因子水平的影响。方法: 将184例哮喘患儿随机分为对照组（常规治疗）90例,观察组（布拉氏酵母菌）94例。两组均按GINA方案治疗。观察组联合布拉氏酵母菌1.0/次,每日2次口服,连用4周。采用ELISA法检测治疗前3组（另设健康组80例）及治疗后2组（观察组和对照组）外周血白介素-10（IL-10）、白介素-17（IL-17）、白介素-5（IL-5）、干扰素-γ（IFN-γ）水平。观察其临床疗效、血清细胞因子及肺功能变化。结果:观察组总有效率(91.49％)明显高于对照组(72.22％),差异有统计学意义(P<0.01)。治疗前两组血清中IL-17、IL-5水平均高于健康组及IL-10、IFN-γ水平低于健康组;治疗后两组IL-17、IL-5均下降（P<0.01,P<0.05 ）,IFN-γ、IL-10上升（P<0.01,P<0.05 ）,但观察组上升或下降幅度明显优于对照组(P<0.01),治疗后组间比较,差异具有统计学意义（P<0.01）。治疗后两组肺功能均有改善,但观察组改善程度明显高于对照组(P<0.01)。 结论:哮喘患儿外周血IL-10、IFN-γ与IL-17、IL-5浓度成负相关。布拉氏酵母菌能有效治疗儿童哮喘,其机制可能与调节Thl/Th2细胞因子的偏移有关。     

硫酸氨基葡萄糖联合参麦注射液治疗膝骨性关节炎的疗效研究

目的: 探讨硫酸氨基葡萄糖联合参麦注射液治疗膝骨性关节炎的疗效。方法: 取膝骨性关节炎患者120例作为研究对象,随机分为联合组和对照组,每组各60例。对照组单纯给予硫酸氨基葡萄糖治疗,联合组给予硫酸氨基葡萄糖（0.5 g/次,3次/天）联合参麦注射液（每次每膝5 mL,每周注射1次）治疗,连续治疗6周,观察两组患者临床疗效。结果: 联合组临床治疗总有效率为91.67%,对照组为78.33%,两组差异有统计学意义（P<0.05）;治疗后,联合组患者膝关节骨性关节炎自评量表（WOMAC）改善较对照组明显,差异有统计学意义（P<0.05）;联合组和对照患者治疗不良反应发生率无显著差异;治疗后,两组患者血清IL-17、IL-18浓度水平明显下降,转化生长因子β（TGF-β）、胰岛素样生长因子1（IGF-1）、成纤维细胞生长因子2（FGF-2）浓度水平明显上升;联合组患者血清IL-17、IL-18浓度水平下降明显优于对照组,TGF-β、IGF-1、FGF-2浓度水平上升明显优于对照组,差异均有统计学意义（P<0.05）。结论:联合组治疗膝骨性关节炎的疗效显著增加,不良反应发生率较低,其作用机制可能与降低血清IL-17、IL-18浓度水平和提高TGF-β、IGF-1、FGF-2浓度水平有关。     

重组人白细胞介素10 对角朊细胞增殖及产生细胞因子的影响

目的: 研究重组人白细胞介素10(rhIL-10) 对无血清培养的角朊细胞增殖和产生细胞因子的影响, 探讨其治疗银屑病的作用机制。方法: 用四甲基偶氮唑蓝比色法(MTT) 测定其对细胞增殖的影响;小鼠胸腺细胞增殖法检测白细胞介素1(IL-1);ELISA 法检测白细胞介素6(IL-6)、白细胞介素8(IL-8) 。结果: 重组人白细胞介素10 抑制角朊细胞增殖与IL-1、IL-6 及IL-8 的分泌, 并呈剂量依赖关系。结论: 重组人白细胞介素10 抑制角朊细胞与细胞因子分泌, 可能是治疗银屑病的作用机理之一。     

The Immunometabolic Roles of Various Fatty Acids in Macrophages and Lymphocytes

Macrophages and lymphocytes demonstrate metabolic plasticity, which is dependent partly on their state of activation and partly on the availability of various energy yielding and biosynthetic substrates (fatty acids, glucose, and amino acids). These substrates are essential to fuel-based metabolic reprogramming that supports optimal immune function, including the inflammatory response. In this review, we will focus on metabolism in macrophages and lymphocytes and discuss the role of fatty acids in governing the phenotype, activation, and functional status of these important cells. We summarize the current understanding of the pathways of fatty acid metabolism and related mechanisms of action and also explore possible new perspectives in this exciting area of research.     

Molecular Biomarkers for Pediatric Depressive Disorders: A Narrative Review

Depressive disorder in childhood and adolescence is a highly prevalent mood disorder that tends to recur throughout life. Untreated mood disorders can adversely impact a patient’s quality of life and cause socioeconomic loss. Thus, an accurate diagnosis and appropriate treatment is crucial. However, until now, diagnoses and treatments were conducted according to clinical symptoms. Objective and biological validation is lacking. This may result in a poor outcome for patients with depressive disorder. Research has been conducted to identify the biomarkers that are related to depressive disorder. Cumulative evidence has revealed that certain immunologic biomarkers including brain-derived neurotrophic factor (BDNF) and cytokines, gastrointestinal biomarkers, hormones, oxidative stress, and certain hypothalamus-pituitary axis biomarkers are associated with depressive disorder. This article reviews the biomarkers related to the diagnosis and treatment of pediatric depressive disorders. To date, clinical biomarker tests are not yet available for diagnosis or for the prediction of treatment prognosis. However, cytokines such as Interleukin-2, interferon-gamma, tumor necrosis factor-alpha, and BDNF have shown significant results in previous studies of pediatric depressive disorder. These biomarkers have the potential to be used for diagnosis, prognostic assessment, and group screening for those at high risk.     

Bacterial Cellulose Membranes Used as Artificial Substitutes for Dural Defection in Rabbits

To improve the efficacy and safety of dural repair in neurosurgical procedures, a new dural material derived from bacterial cellulose (BC) was evaluated in a rabbit model with dural defects. We prepared artificial dura mater using bacterial cellulose which was incubated and fermented from Acetobacter xylinum. The dural defects of the rabbit model were repaired with BC membranes. All surgeries were performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. All animals were humanely euthanized by intravenous injection of phenobarbitone, at each time point, after the operation. Then, the histocompatibility and inflammatory effects of BC were examined by histological examination, real-time fluorescent quantitative polymerase chain reaction (PCR) and Western Blot. BC membranes evenly covered the surface of brain without adhesion. There were seldom inflammatory cells surrounding the membrane during the early postoperative period. The expression of inflammatory cytokines IL-1β, IL-6 and TNF-α as well as iNOS and COX-2 were lower in the BC group compared to the control group at 7, 14 and 21 days after implantation. BC can repair dural defects in rabbit and has a decreased inflammatory response compared to traditional materials. However, the long-term effects need to be validated in larger animals.     

急性酒精中毒对家兔失血性休克炎症介质的影响

目的：探讨急性酒精中毒对失血性休克后早期炎症反应的影响。方法将40只健康新西兰大耳白兔按随机数字表法分为4组,每组10只,分别为对照组（A 组）、失血性休克组（B 组）、急性酒精中毒组（C 组）和急性酒精中毒合并失血性休克组（D 组）,制作相应动物模型。监测达到休克所需的放血量和恢复伤前血压的补血、补液总量,分别于休克前、休克中、休克复苏后1 h、休克复苏后3 h 测定血清中 TNF-α、IL-6、IL-10含量。结果1）D 组的放血量要小于 B 组,复苏需要更多液体;2）与 A 组比较,B 组及 D 组血清 TNF-α、IL-6、IL-10含量均显著升高（P ＜0．01）;3）与 B 组比较,D 组各观察时点血清 TNF-α、IL-6、IL-10含量均显著升高（P ＜0．05或 P ＜0．01）。结论失血性休克后早期炎症因子的表达升高,急性酒精中毒能加重炎症介质的释放。