Guided curation of semistructured data in collaboratively-built knowledge bases

The collaborative curation of semistructured knowledge has become a popular paradigm on the web and also within enterprises. In such knowledge bases a common structure of the stored information is crucial for providing efficient and precise search facilities. However, the task of refining, extending and homogenizing knowledge and its structure is very complex. In this article we present two paradigms for the simplification of this task by providing guidance mechanisms to the user. Both paradigms aim at combining the power of automated extraction algorithms with the semantic awareness of human users to accomplish this refinement task.     

The effect of probiotics (Lactobacillus rhamnosus HN001, Lactobacillus paracasei LPC-37, and Lactobacillus acidophilus NCFM) on the availability of minerals from Dutch-type cheese

The use of probiotic cultures in the production of Dutch-type cheeses did not lead to significant changes in their chemical composition but it lowered their acidity. The availability of calcium and magnesium analyzed by in vitro enzymatic hydrolysis was 19 and 35%, respectively; the availability of phosphorus was significantly higher, at >90%. The use of probiotic cultures significantly increased the availability of calcium (~2.5%), phosphorus (~6%), and magnesium (~18%). The in vitro method supports accurate determination of the effect of the Lactobacillus spp. cultures on the availability of mineral compounds ingested with Dutch-type cheese.     

Influence of malting and lactic acid fermentation on functional bioactive components in cereal‐based raw materials: a review paper

Driven by increased health awareness among consumers, the production of foods and beverages enriched with functional bioactive components is gaining more attention. Malting and lactic acid fermentation are biotechnological processes having potential for producing functional foods and beverages. Due to various biochemical and enzymatic induced changes in raw materials, malting of cereal grains and probiotic lactic acid fermentation of plant‐based media increases the nutritional quality of treated raw materials. The improved nutritional quality is attributed to the accumulation of functional bioactive components along with the degradation of anti‐nutritional components. The selection of raw materials and process parameters are important factors to be considered for increasing the functional bioactive components such as dietary fibres, antioxidants and probiotics. This review article reports the current knowledge on the changes of bioactive components during malting and lactic acid fermentation using probiotic bacterial strains. Process parameters which affect the concentration of bioactive components in raw materials will also be described.     

Production of human growth hormone by Lactococcus lactis

A synthetic gene coding for human growth hormone was expressed in Lactococcus lactis. The presence of the recombinant protein was assayed and quantified using ELISA tests. Human growth hormone was detected at high concentrations and displayed a biological activity similar to the one shown by commercial human growth hormone.     

miR-34a通过调控雄激素受体基因抑制前列腺癌细胞系LNCaP增殖

目的：探究miR-34a对雄激素受体（AR）基因的调控作用及对前列腺癌（PCa）LNCaP细胞增殖、凋亡的影响。方法：收集2016年10月至2019年9月本院泌尿外科行前列腺穿刺活检确诊为PCa患者组织标本36例,另取同期行手术治疗切除的良性前列腺增生（BPH）组织标本41例。体外培养LNCaP细胞,分别转染miR-34a mimics（mimics组）,miR-34a mimic NC（NC组）,设正常生长细胞为空白对照组（BC组）,另在mimics组基础上转染AR过表达（AR过表达组）载体及其对照（AR对照组）,采用CCK-8试剂盒检测细胞活性,Annexin V-FITC/PI双染色流式细胞凋亡检测试剂盒检测细胞凋亡率,实时定量PCR（RT-qPCR）检测miR-34a及AR mRNA表达水平,免疫印迹法检测AR蛋白、细胞周期蛋白D1（cyclinD1）及原癌基因产物（c-Mcy）、细胞凋亡相关蛋白（Bcl-2、Bax、capase-3）的表达水平。结果：与BPH组织比较,PCa组织中miR-34a表达水平显著降低（P<0.05）,AR mRNA及蛋白表达水平均显著增加（P<0.05）;与BC、NC组比较,mimics组LNCap细胞miR-34a表达水平、细胞凋亡率、Bax蛋白表达水平显著增加（P<0.05）,AR、Cyclin D1、Bcl-2、Bax蛋白表达水平显著降低（P<0.05）,同一时间LNCap细胞活力均显著降低（P<0.05）;双荧光素酶实验结果显示,miR-34a与AR可能存在一定的调控关系,过表达AR基因可逆转miR-34a mimics对LNCaP细胞增殖抑制,促进凋亡作用。 结论：miR-34a可能通过调控AR抑制前列腺癌LNCaP细胞增殖,促进其凋亡。     

重组人促红细胞生成素对人乳腺癌MDA-MB-231细胞增殖的影响及其作用机制研究

目的 探讨重组人促红细胞生成素（recombinant human erythropoietin,rh-EPO）对人乳腺癌MDA-MB-231细胞增殖的影响及其作用机制.方法 将人乳腺癌 MDA-MB-231 细胞进行培养.传至5～6代,细胞生长状态稳定后,收集人乳腺癌 MDA-MB-231细胞用于MTT实验.采用MTT法检测 5 组（阴性对照组、rh-EPO A 组、rh-EPO B组、rh-EPO C 组和rh-EPO D 组）MDA-MB-231细胞增殖的情况.用10 μmol·L-1p38MAPK抑制剂SB203580、ERK抑制剂U0126、JNK抑制剂SP600125和NF-κB 抑制剂PDTC预处理人乳腺癌 MDA-MB-231 细胞后,用MTT法检测经100、200、300和400 U·mL-1的rh-EPO（PDTC＋EPO 组、SB203580＋EPO 组、SP600125＋EPO组和U0126＋EPO组）诱导后细胞增殖的情况.结果 阴性对照组、rh-EPO A 组、rh-EPO B组、rh-EPO C 组和rh-EPO D 组 72 h PI值分别为：1.000 0±1.000 0、1.231 8±0.133 0、1.323 9±0.136 0、1.351 7±0.146 0和1.423 1±0.084 0;96 h PI值分别为：1.000 0±1.000 0、1.352 5±0.036 0、1.359 7±0.112 0、1.387 2±0.063 0和1.410 8±0.060 0.rh-EPO A 组、rh-EPO B组、rh-EPO C 组和rh-EPO D 组 72、96 h PI值与阴性对照组比较差异均有统计学意义（均P＜0.05）.PDTC＋EPO 组、SB203580＋EPO 组72、96 h PI值均较EPO组明显降低（均P＜0.05）,SP600125＋EPO组、U0126＋EPO组72、96 h PI值与EPO组比较差异均无统计学意义（均P＞0.05）.结论 rh-EPO可能是通过NF-κB、MAPK传导通路发挥效应,促进人乳腺癌MDA-MB-231细胞增殖.     

新型多功能季铵化抗菌材料的制备与应用

综述了近年来在抗污、超滑/超亲水、可降解、促凝血、促细胞生长等新型多功能季铵化抗菌材料的分子设计和潜在应用方面取得的研究进展,指出在抗菌的同时并具有以上所提及的多种功能是抗菌材料发展的一大趋势。举例说明通过原子转移自由基聚合（ATRP）技术,合成的星状聚合物（POSS-g-PDMA）,在季铵化后具有了极高的抗菌活性、相对非血溶性和可降解性。高度季铵化的基于二甲基癸基铵壳聚糖-接枝-聚(乙二醇)甲基丙烯酸酯(DMDC-Q-g-EM)和聚(乙二醇)二丙烯酸酯的抗菌水凝胶兼具抗菌和抗真菌活性、生物相容性和可重复使用性。最后对新型多功能季铵化抗菌材料的未来发展方向进行了展望。