Delta V系统在甲乙酮装置中的应用

通过Delta V系统在兰州石油化工公司甲乙酮装置的应用，详细介绍了Deha V系统的优点及相关组态和经验。     

Research on Development Strategy for Rubber Industry Power Completed Within the Year

On the Annual Rubber Industry Conference 2014 held in Qingdao last weekend, the reporter learned that the Research on Development Strategy for Rubber Industry Power organized and compiled by China Rubber Industry Association （hereinafter referred to as CRIA） had entered the stage of concrete compilation and was planned to be completed and published in October this year.     

基于MP3的新型公交车自动报站系统

介绍了一种基于MP3的新型公交车自动报站系统的设计原理，同时提供了一种以89C51SND1单片机为核心，利用芯片内集成的解码器进行MP3语音播放的解决方案，该方案亦为目前许多MP3播放器所采用。     

人二型大麻受体基因的pIRES2-EGFP质粒的构建及其真核表达

目的：构建含有人二型大麻素受体（CB2）基因的真核表达载体,并实现其在HEK293细胞的表达。方法：首先采用PCR方法从含有CB2基因的质粒pcDNA3.1（＋）-CB2中扩增获得人的CB2基因,再采用基因重组技术将CB2的DNA片段插入真核表达载体pIRES2-EGFP。将获得的pIRES2-EGFP-CB2重组子转染HEK293细胞,应用荧光显微镜观察EGFP的表达情况,RT-PCR和报告基因检测CB2的表达情况。结果：酶切和测序结果验证真核表达载体pIRES2-EGFP-GB2构建成功。可在荧光显微镜下观察到转染HEK293细胞表达的绿色荧光;RT-PCR和双荧光素酶报告基因法检测证明CB2蛋白在真核细胞中成功表达;CB2受体激动剂JWH-015能显著逆转腺苷酸环化酶激动剂forskolin诱发的荧光素酶活性增加。结论：成功构建了真核表达载体pIRES2-EGFP-CB2,为进一步研究CB2基因的生理学和病理生理学功能及调控机制奠定了实验基础。     

广播记者的新闻敏感性与受众心理分析

作为一名广播记者,在日常工作过程中重视自身新闻敏感性建设已经成为职业的必要素养,对于个人的成长与职业进步显得十分重要。只有不断提升自身的新闻敏感性,才能够在纷繁错杂的信息世界中获取更有价值、更有现实意义的新闻报道方向,来提高新闻报道整体的实用价值,从而推动广播新闻报道的长期发展。     

基于XScale和Windows CE的智能公交报站器的设计与实现

针对我国快速发展的公交运行系统高度自动化的需求,讨论了在嵌入式Windows CE.net系统平台下利用32位RISC处理器XScale和Rikaline公司的GPS-24模块实现智能报站器的步骤和方法。详细介绍了实时嵌入式操作系统Windows CE.net的特点、内核的定制以及在嵌入式处理器XScale上的移植过程。在硬件上,讨论了使用串口在GPS模块与处理器之间通信的方法;在软件上,讨论了采用实时多线程编程方式分配系统任务的方法,将系统任务分配为GPS信息接收解析、LCD显示和语音报站3个子线程,实现多任务的操作。     

Single cell reporter assay using cell surface displayed Vargula luciferase

Reporter genes such as firefly luciferase are common tools to monitor gene expression in various systems. As reporter gene represents the expression level of the gene of interest with its enzyme activity, firefly luciferase is most frequently used because its luminescent activity is highly sensitive and less time consumable for assay. However, since firefly luciferase is expressed internally in the cell, lysis of the cell is a critical step, and thus it is difficult to monitor the gene expression level continuously. In this report, we utilized secretive Vargula hilgendorfii luciferase modified to cell surface displayed one by fusing with human EGFR transmembrane sequence. This modified Vargula luciferase was expressed on cell surface without losing its bioluminescent activity. Co-transfection with secretive alkaline phosphatase showed that the behaviors of cell surface displayed Vargula luciferase and secretive alkaline phosphatase are comparable to each other. Furthermore, the luminescence of a single cell expressing cell surface displayed Vargula luciferase can be monitored by using photon counting CCD camera, which indicates that this reporter gene can monitor gene expression in a single cell without cell lysis.     

周恩来与中外记者西北参观团

抗战后期,中外记者西北参观团访问延安是中国共产党外事工作中的一件大事。文章对周恩来与记者团的形成及其访问成功之间的关系作了分析和评价。