BET Inhibition Upregulates SIRT1 and Alleviates Inflammatory Responses

Control of histone acetylation is a part of the epigenetic mechanism that regulates gene expression and chromatin architecture. The members of the bromodomain and extra terminal domain (BET) protein family are a group of epigenetic readers that recognize histone acetylation, whereas histone deacetyl‐ ases such as sirtuin 1 (SIRT1) function as epigenetic erasers. We observed that BET inhibition by the specific inhibitor JQ1 upregulated SIRT1 expression and activated SIRT1. Moreover, we observed that BET inhibition functionally reversed the pro‐inflammatory effect of SIRT1 inhibition in a cellular lung disease model. SIRT1 activation is desirable in many age‐related, metabolic and inflammatory diseases; our results suggest that BET protein inhibition would be beneficial in treatment of those conditions. Most importantly, our findings demonstrate a novel mechanism of SIRT1 activation by inhibition of the BET proteins.     

Supramolecular Ligands for Histone Tails by Employing a Multivalent Display of Trisulfonated Calix[4]arenes

Post‐translational modification of histone tails plays critical roles in gene regulation. Thus, molecules recognizing histone tails and controlling their epigenetic modification are desirable as biochemical tools to elucidate regulatory mechanisms. There are, however, only a few synthetic ligands that bind to histone tails with substantial affinity. We report CA2 and CA3, which exhibited sub‐micromolar affinity to histone tails (especially tails with a trimethylated lysine). Multivalent display of trisulfonated calix[4]arene was important for strong binding. CA2 was applicable not only to synthetic tail peptides but also to endogenous histone proteins, and was successfully used to pull‐down endogenous histones from nuclear extract. These findings indicate the utility of these supramolecular ligands as biochemical tools for studying chromatin regulator protein and as a targeting motif in ligand‐directed catalysis to control epigenetic modifications.     

轴向约束混凝土短柱受火后抗震性能试验研究

为研究高温下混凝土短柱的持荷和端部轴向约束对受火后混凝土短柱抗震性能的影响,进行了10根受火1h后混凝土短柱和2根未受火对比柱的拟静力试验,分析了轴向约束、持荷、轴压比和剪跨比等因素对受火后混凝土短柱的破坏形态、峰值荷载、延性和耗能的影响.结果表明:高温下未持荷短柱、高温下持荷短柱和高温下轴向约束短柱受火1h后峰值荷载均显著降低,是未受火时的67.7％ ～ 75.3％.高温下初始轴压比为0.2时,高温下轴向约束短柱、高温下持荷短柱的高温后峰值荷载分别为高温下未持荷短柱的92.0％ ～ 99.9％、99.5％～102.2％.随着轴压比的增大,高温下轴向约束混凝土短柱的附加轴压力减小,高温后受剪承载力增大.当受火时间不超过1h且轴压比不超过0.3时,受火后混凝土短柱的受剪承载力评定可不考虑高温下持荷和轴向约束的影响.     

高温后钢筋再生混凝土轴压短柱受力性能试验研究

为了研究高温后钢筋再生混凝土轴压短柱的受力性能,以再生粗骨料取代率、温度、最高温度持时、混凝土强度和箍筋间距为变化参数,设计40个试件(其中高温后试件32个、常温对比试件8个)进行静力加载试验.观察了高温后钢筋再生混凝土轴压短柱的破坏形态,获得了其承载力、刚度、位移延性系数和耗能等力学性能指标,并对不同变化参数试件的力学性能指标进行分析,探讨了高温后钢筋再生混凝土轴压短柱的承载力计算方法.研究结果表明:随着温度升高,钢筋再生混凝土柱的外观颜色逐渐由青灰色转变为灰白色,其质量烧失率逐渐增大,试件的承载力和初始刚度逐渐降低;再生粗骨料取代率的大小对高温后钢筋再生混凝土轴压短柱的承载力和初始刚度的影响不大;混凝土强度等级越高的试件,其遭受高温后的承载力和初始刚度也越大;最高温度持时和箍筋间距的变化对高温后钢筋再生混凝土轴压短柱力学性能影响不大.     

适度设计——中国联合工程公司国机研发中心设计

结合中国联合工程公司国机研发中心设计,提出了"适度设计"的概念,探讨了建筑表达、高效空间、人文关怀以及低碳技术等内容。     

城市综合体火灾自动报警系统设计

介绍某在建城市综合体项目的火灾自动报警系统依据GB 50116-2013《火灾自动报警系统设计规范》进行重新设计和调整的情况,如重新配置了火灾报警控制器、增设总线短路隔离器、地下车库改为点型感烟火灾探测器等,使消防系统设计更安全、合理、可靠。     

变压器供电系统电动机启动电压降计算

简介与变压器供电系统电机启动电压计算有关的参数和概念;运用"功率法"和"阻抗法"对工程案例进行计算;对工程设计中常遇到的问题进行讨论。     

从三维模型到三维数字地图的突破——解读浙江省《三维数字地图技术规范》

三维数字产品已成为基础地理信息数据的重要组成部分,从三维模型到三维数字地图是个重大突破。本文简述了浙江省《三维数字地图技术规范》(DB33/T934-2014)的内容,分析了该标准与国家行业标准的关系以及在模型分类和编码、地图分幅和编号、比例尺、数学基础、精度和表达等方面的创新点。在实施时宜结合实际,选择产品的类型,制定相应的技术指标。     

浅埋房式采空区集中煤柱下综采动载控制研究

石圪台煤矿31201综采工作面过上方房式采空区集中煤柱后,上煤层顶板运动造成下煤层综采面大量支架压死。通过建立过煤柱阶段覆岩结构模型,推导出工作面在出煤柱时的结构必然失稳是动载力源,辅以数值模拟揭示了动载矿压大于支架工作阻力的压架机理,由此提出预先爆破煤柱、释放岩层能量的治理方案。同步进行地面钻孔位移监测、从地面和井下钻孔布置传感器进行微震监测,掌握矿压显现特征,预警了动压危险。地表钻孔窥视说明了过煤柱动载显然要大于进入煤柱期间的动载,多点位移观测和微震观测说明预警爆破措施可有效防治动载矿压。